Utilization of ion-exchange chromatography in the isolation and colorimetric assay of p-aminophenyl glucuronide derivatives in human urine

1970 ◽  
Vol 29 (3) ◽  
pp. 421-427 ◽  
Author(s):  
Gerald M. Lower ◽  
Sharon B. Murphy ◽  
George T. Bryan
Keyword(s):  
Ion Exchange ◽  
Human Urine ◽  
Colorimetric Assay ◽  
Ion Exchange Chromatography ◽  
Exchange Chromatography

Isolation of 2-Acetamido-1-β-(L-β-Aspartamido)-1,2-Dideoxy-D-Glucose from Normal Human Urine

1972 ◽  
Vol 18 (9) ◽  
pp. 951-955 ◽  
Author(s):  
Barbara O’Neill Rowley ◽  
Paul B Hamilton
Keyword(s):  
Ion Exchange ◽  
Human Urine ◽  
Ion Exchange Chromatography ◽  
Exchange Chromatography ◽  
Normal Individuals ◽  
Normal Human ◽  
Elution Behavior ◽  
Solvent Systems ◽  
And Migration ◽  
Layer Chromatography

Abstract A glycopeptide was isolated from normal human urine by fractionation on a column of Sephadex G-10 and preparative ion-exchange chromatography. Elution behavior during ion-exchange chromatography in two different solvent systems, amino acids formed upon hydrolysis, and migration on high-voltage electrophoresis and thin-layer chromatography were essentially identical for this substance and for authentic 2-acetamido-l-β-(L-β-aspartamido)-1,2-dideoxy-D-glucose. A technique was developed to permit analytical-scale fractionation of individual urines followed by analysis for this glycopeptide; urine from two normal individuals contained 7 and 11 µmol of 2-acetamido-1-β-(L-β-aspartamido)-1,2-dideoxy-D-glucose per liter.

Comparison of a Colorimetric Assay for Glycosylated Hemoglobin with Ion-exchange Chromatography

Diabetes ◽  
1979 ◽  
Vol 28 (12) ◽  
pp. 1120-1125 ◽  
Author(s):  
R. E. Pecoraro ◽  
R. J. Graf ◽  
J. B. Halter ◽  
H. Beiter ◽  
D. Porte
Keyword(s):  
Ion Exchange ◽  
Colorimetric Assay ◽  
Glycosylated Hemoglobin ◽  
Ion Exchange Chromatography ◽  
Exchange Chromatography

FURTHER STUDIES ON THE NATURE OF THE IMMUNOREACTIVE LUTEINIZING HORMONE-RELEASING HORMONE (LH-RH)-LIKE PEPTIDE IN HUMAN URINE

1975 ◽  
Vol 78 (1) ◽  
pp. 232-238 ◽  
Author(s):  
S. L. Jeffcoate ◽  
Diane T. Holland
Keyword(s):  
Luteinizing Hormone ◽  
Ion Exchange ◽  
Human Urine ◽  
Chemical Nature ◽  
Ion Exchange Chromatography ◽  
Exchange Chromatography ◽  
Urine Samples ◽  
Luteinizing Hormone Releasing Hormone ◽  
Releasing Hormone ◽  
Lh Rh

ABSTRACT The chemical nature of the immunoreactive LH-RH-like peptide found in human urine has been investigated using ion-exchange chromatography on carboxymethyl (CM)-cellulose and a radioimmunoassay for LH-RH. A single immunoreactive substance was found in urine after LH-RH administration and in urine samples from untreated subjects. This substance did not have the mobility of either the synthetic decapeptide nor the 3–10 octapeptide on CM-cellulose and the evidence suggests that it may be the 2–10 nonapeptide of LH-RH.

Fractionation of Urine to Allow Desmosine Analysis by Radioimmunoassay

1992 ◽  
Vol 29 (1) ◽  
pp. 72-78 ◽  
Author(s):  
Barry Starcher ◽  
Marti Scott
Keyword(s):  
Ion Exchange ◽  
Affinity Chromatography ◽  
Human Urine ◽  
Gel Filtration ◽  
Ion Exchange Chromatography ◽  
Exchange Chromatography ◽  
The Other ◽  
Normal Adult ◽  
Average Adult

The present study was designed to re-evaluate the radioimmunoassay for desmosine in urine, which is currently used as a measure of elastin metabolism. Using ion exchange chromatography, gel filtration and affinity chromatography it was shown that at least five other compounds in hydrolysates of human urine competed for desmosine in the RIA. Fractionating the urine prior to hydrolysis with acetone removed one of the major contaminants. The other contaminants could subsequently be removed by extracting the urine hydrolysate with a mixture of chloroform/ethanol (60:40). Samples from nine normal adult urines showed that an average of 45% of the RIA competing material in unfractionated urine was not desmosine. The final extracted residue retained all of the desmosine and only 16% of the original solids. The average adult urine contains approximately 50 pmol desmosine/mg creatinine, reflecting a daily turnover of between 3 and 4 mg of elastin per day.

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