Comparison of procedures for extracting free amino acids from polymorphonuclear lekocytes.

1976 ◽  
Vol 22 (10) ◽  
pp. 1618-1622 ◽  
Author(s):  
Y Houpert ◽  
P Tarallo ◽  
G Siest
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Quantitative Determination ◽  
Free Amino Acids ◽  
Free Amino ◽  
Polymorphonuclear Leukocytes ◽  
Extraction Procedure ◽  
Intracellular Pool ◽  
Human Polymorphonuclear Leukocytes

Abstract We studies five methods for extracting amino acids from human polymorphonuclear leukocytes. Both the use of cell lysis and of a deproteinizing agent interfere with quantitative determination of the amino acids, basic amino acids being the most sensitive to the extraction procedure. Among the methods used, disruption of the cells by freezing-thawing is the best method for extracting all the amino acids. Taurine is the only amino acid extracted in the same amount by all the methods studied, and it represents half of the intracellular pool.

Quantitative determination of free amino acids with automatic amino acid analyser in liver cirrhosis blood plasma

1966 ◽  
Vol 1 (3) ◽  
pp. 75-75
Author(s):  
S. Tanaka ◽  
K. Hattori ◽  
Y. Katoo ◽  
S. Suga ◽  
A. Ishiwara ◽  
...  
Keyword(s):  
Amino Acids ◽  
Liver Cirrhosis ◽  
Amino Acid ◽  
Quantitative Determination ◽  
Blood Plasma ◽  
Free Amino Acids ◽  
Free Amino ◽  
Amino Acid Analyser

Determination of Free Amino Acids in Coated Foods by GC–MS: Optimization of the Extraction Procedure by Using Statistical Design

2013 ◽  
Vol 7 (1) ◽  
pp. 172-180 ◽  
Author(s):  
T. Pérez-Palacios ◽  
A. Melo ◽  
S. Cunha ◽  
I. M. P. L. V. O. Ferreira
Keyword(s):  
Amino Acids ◽  
Free Amino Acids ◽  
Free Amino ◽  
Extraction Procedure ◽  
Statistical Design

scholarly journals Free amino acids of testes. Concentrations of free amino acids in the testes of several species and the precursors of glutamate and glutamine in rat testes in vivo

1973 ◽  
Vol 132 (3) ◽  
pp. 353-359 ◽  
Author(s):  
Isa K. Mushahwar ◽  
Roger E. Koeppe
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Guinea Pig ◽  
Free Amino Acids ◽  
Free Amino ◽  
Ammonium Acetate ◽  
Testicular Tissue ◽  
Species Variation

Determination of the free amino acid and lactate content of testicular tissue in rat, guinea pig, rabbit, cat, gerbil, hamster, chicken and bullfrog indicates a substantial species variation. Insulin hypoglycaemia and ammonium acetate toxicity changes the concentration of several free amino acids of rat testes. 14C radioactivity from labelled acetate and ethanol is rapidly incorporated into some of the free amino acids of rat testes in vivo, whereas incorporation from [14C]glucose is relatively slow. These results have been compared with those obtained from similar studies with rat brain. In contrast to brain, there is no evidence for glutamate compartmentation in testes.

scholarly journals Determination of trans Vitamin K1 in Infant and Medical Nutritional Products Using AOAC Method 999.15 with Modified Preparation and Extraction Procedures and C30 Bonded Phase Chromatography: Single-Laboratory Validation

2010 ◽  
Vol 93 (2) ◽  
pp. 650-662 ◽  
Author(s):  
Karen J Schimpf ◽  
Linda B Thompson ◽  
Daniel J Schmitz
Keyword(s):  
Amino Acids ◽  
Chromatographic Separation ◽  
Free Amino Acids ◽  
Free Amino ◽  
Extraction Procedure ◽  
Vitamin K1 ◽  
Infant Formulas ◽  
Aoac Method ◽  
Single Laboratory

Abstract Modifications were made to AOAC Official MethodSM 999.15 to extend its applicability to specialty infant formulas containing hydrolyzed proteins and free amino acids, and to medical and adult nutritional products. Minor changes to the sample preparation procedure and chromatographic separation improved vitamin K1 recoveries and reduced chromatographic interferences in these types of matrixes. Currently AOAC Method 999.15 is applicable only to the determination of total vitamin K1 (phylloquione) in infant formula and milk (fluid, ready-to-feed, and powdered) containing >1 g vitamin K1/100 g solids. AOAC Method 999.15 recoveries of vitamin K1 were improved by altering sample sizes, extraction solvents and amounts, and the reagent addition order and amount of water or aqueous solutions added. The chromatographic separation of vitamin K1 in medical nutritional products containing canola and marine oils was improved, and trans vitamin K1 was separated from the biologically inactive cis isomer in all products with a C30 3 m column and a 100 methanol mobile phase. With these modifications to the extraction procedure and chromatographic separation, AOAC Method 999.15 demonstrated acceptable precision and accuracy for the quantitation of trans vitamin K1 in specialty infant formulas containing hydrolyzed proteins and free amino acids, and medical and adult nutritional products. A single-laboratory validation of these minor modifications was completed. Fourteen different product matrixes were analyzed during validation. The intermediate precision averaged 4.15 RSD (range 2.525.81 RSD), and recovery data averaged 100.1 (range 92.2109).

scholarly journals Determination of free amino acid and biogenic amine contents of hungarian sparkling wines

2004 ◽  
Vol 22 (SI - Chem. Reactions in Foods V) ◽  
pp. S287-S289 ◽  
Author(s):  
L. Simon-Sarkadi ◽  
E. Szőke ◽  
A. Kerekes
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Biogenic Amines ◽  
Free Amino Acid ◽  
Biogenic Amine ◽  
Free Amino Acids ◽  
Free Amino ◽  
Exchange Chromatography ◽  
Sparkling Wine

Comparative study was conducted on the basis of free amino acids and biogenic amines of Hungarian sparkling wines originated from 3 producers (Törley, Hungária, Balaton Boglár). Determination of amino acids and biogenic amines was accomplished by ion-exchange chromatography using an amino acid analyser. The dominant free amino acids in sparkling wines were proline and arginine and the major biogenic amine was spermidine. Based on results of chemometric analyses, free amino acid and biogenic amine contents seemed to be closely related to quality and the technology of sparkling wine making.

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