Quantitative determination of free amino acids with automatic amino acid analyser in liver cirrhosis blood plasma

1966 ◽  
Vol 1 (3) ◽  
pp. 75-75
Author(s):  
S. Tanaka ◽  
K. Hattori ◽  
Y. Katoo ◽  
S. Suga ◽  
A. Ishiwara ◽  
...  
Keyword(s):  
Amino Acids ◽  
Liver Cirrhosis ◽  
Amino Acid ◽  
Quantitative Determination ◽  
Blood Plasma ◽  
Free Amino Acids ◽  
Free Amino ◽  
Amino Acid Analyser

Comparison of procedures for extracting free amino acids from polymorphonuclear lekocytes.

1976 ◽  
Vol 22 (10) ◽  
pp. 1618-1622 ◽  
Author(s):  
Y Houpert ◽  
P Tarallo ◽  
G Siest
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Quantitative Determination ◽  
Free Amino Acids ◽  
Free Amino ◽  
Polymorphonuclear Leukocytes ◽  
Extraction Procedure ◽  
Intracellular Pool ◽  
Human Polymorphonuclear Leukocytes

Abstract We studies five methods for extracting amino acids from human polymorphonuclear leukocytes. Both the use of cell lysis and of a deproteinizing agent interfere with quantitative determination of the amino acids, basic amino acids being the most sensitive to the extraction procedure. Among the methods used, disruption of the cells by freezing-thawing is the best method for extracting all the amino acids. Taurine is the only amino acid extracted in the same amount by all the methods studied, and it represents half of the intracellular pool.

scholarly journals Free amino acids of testes. Concentrations of free amino acids in the testes of several species and the precursors of glutamate and glutamine in rat testes in vivo

1973 ◽  
Vol 132 (3) ◽  
pp. 353-359 ◽  
Author(s):  
Isa K. Mushahwar ◽  
Roger E. Koeppe
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Guinea Pig ◽  
Free Amino Acids ◽  
Free Amino ◽  
Ammonium Acetate ◽  
Testicular Tissue ◽  
Species Variation

Determination of the free amino acid and lactate content of testicular tissue in rat, guinea pig, rabbit, cat, gerbil, hamster, chicken and bullfrog indicates a substantial species variation. Insulin hypoglycaemia and ammonium acetate toxicity changes the concentration of several free amino acids of rat testes. 14C radioactivity from labelled acetate and ethanol is rapidly incorporated into some of the free amino acids of rat testes in vivo, whereas incorporation from [14C]glucose is relatively slow. These results have been compared with those obtained from similar studies with rat brain. In contrast to brain, there is no evidence for glutamate compartmentation in testes.

scholarly journals Possibilities for prediction of congenital fetal malformations and chromosomal abnormalities based on the determination of the levels of free amino acids and their nitrogen-containing derivatives in blood plasma of pregnant women

2020 ◽  
pp. 152-158
Author(s):  
L. N. Keda ◽  
A. V. Naumov ◽  
V. YU. Smirnov
Keyword(s):  
Amino Acids ◽  
Blood Plasma ◽  
Pregnant Women ◽  
Free Amino Acids ◽  
Free Amino ◽  
Chromosomal Abnormalities ◽  
Group I ◽  
Fetal Malformations ◽  
In Blood Plasma

Objective: to determine the possibilities for prediction and diagnosis of congenital fetal malformations and chromosomal abnormalities on the basis of the study of the levels of amino acids and their nitrogen-containing derivatives in blood plasma of pregnant women with pathological conditions requiring artificial termination of pregnancy. Material and methods. The content of free amino acids and their nitrogen-containing derivatives was studied in 104 pregnant women having congenital malformations and chromosomal abnormalities in their fetuses at 13-22 weeks` gestation (group I) and 25 women with physiological pregnancy (group II). The amino acid level was determined by the high-performance liquid chromatography method. Results. The levels of 14 out of the 26 studied amino acids in the blood plasma of the pregnant women of group I were statistically higher than those of the women in group II. ROC analysis was used to determine six amino acids (glycine, α-aminobutyric acid, hydroxylysine, glutamic acid, citrulline, serine) and their threshold values which with high accuracy (85.3 %) allow of predicting congenital fetal malformations and chromosomal abnormalities. A prognostic model making it possible to determine high probability of congenital fetal anomalies based on the determination of the concentration of 5-hydroxytryptophan, glycine, asparagine, and serine in blood plasma of pregnant women has been developed. Conclusion. The study of the levels of amino acids and their nitrogen-containing derivatives in plasma of pregnant women at 13-22 weeks` gestation can be used for prenatal diagnosis of congenital fetal malformations and chromosomal abnormalities, as well as used as an additional criterion for making the decision on the necessity for artificial termination of pregnancy upon fetal medical indications.

scholarly journals FREE AMINO ACIDS OF BLOOD PLASMA AS AN INTEGRAL PARAMETER OF METABOLIC DISORDERS IN A LONG-TERM INTAKE OF SMALL DOSES OF LEAD ACETATE INTO THE ORGANISM

2017 ◽  
pp. 67-71
Author(s):  
V. M. Sheybak ◽  
A. Y. Pavliukovets ◽  
V. Yu. Smirnov
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Blood Plasma ◽  
Free Amino Acids ◽  
Free Amino ◽  
Lead Acetate ◽  
Male Rats ◽  
Integral Parameter ◽  
In Blood Plasma

Objective: to analyze the pool of free amino acids and their nitrogen-containing metabolites of blood plasma of animals being administered lead acetate for 21 or 28 days. Material and methods. The experiments were carried out on white male rats with the initial weight of 140-160 g. During the experiment the animals received a total amount of 0.67 g of lead acetate (21 days), the second group - 1.12 g (28 days). The determination of free amino acids in blood plasma was performed by the method of reversed-phase HPLC. Results. As a result of the research it has been found out that the most significant contribution to the formation of the amino acid imbalance in blood plasma as a result of the long-term intake of lead acetate according to the value of the Fisher test is caused by changes in the concentrations of α-aminobutyric acid, isoleucine, aspartate, arginine, glycine, serine, histidine, and methionine. Conclusion. The long-term intake of lead acetate with drinking water leads to a statistically significant increase of general content of free amino acids and their nitrogen-containing metabolites, as well as to a decrease of the concentration the essential amino acid threonine in blood plasma.

scholarly journals Determination of free amino acid and biogenic amine contents of hungarian sparkling wines

2004 ◽  
Vol 22 (SI - Chem. Reactions in Foods V) ◽  
pp. S287-S289 ◽  
Author(s):  
L. Simon-Sarkadi ◽  
E. Szőke ◽  
A. Kerekes
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Biogenic Amines ◽  
Free Amino Acid ◽  
Biogenic Amine ◽  
Free Amino Acids ◽  
Free Amino ◽  
Exchange Chromatography ◽  
Sparkling Wine

Comparative study was conducted on the basis of free amino acids and biogenic amines of Hungarian sparkling wines originated from 3 producers (Törley, Hungária, Balaton Boglár). Determination of amino acids and biogenic amines was accomplished by ion-exchange chromatography using an amino acid analyser. The dominant free amino acids in sparkling wines were proline and arginine and the major biogenic amine was spermidine. Based on results of chemometric analyses, free amino acid and biogenic amine contents seemed to be closely related to quality and the technology of sparkling wine making.

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