Multicomponent analysis in clinical chemistry by use of rapid scanning fluorescence spectroscopy.

1976 ◽  
Vol 22 (9) ◽  
pp. 1483-1492 ◽  
Author(s):  
I M Warner ◽  
J B Callis ◽  
E R Davidson ◽  
G D Christian
Keyword(s):  
Least Squares ◽  
Clinical Laboratory ◽  
Clinical Chemistry ◽  
Emission Spectra ◽  
Fluorescence Analysis ◽  
Light Level ◽  
Excitation Spectra ◽  
Least Squares Fit ◽  
Reduction Strategies ◽  
Complete Set

Abstract To be useful in the clinical laboratory, multicomponent fluorescence analysis requires both the rapid measurement of the fluorescence intensity at a variety of excitation and emission wavelengths and the unambiguous reduction of the data by efficient algorithms. The Video Fluorometer, which exploits the multi-channel capability of a low-light-level television sensor to simultaneously acquire excitation and emission spectra, can meet the first requirement. For example, a complete set of emission and excitation spectra for perylene can be obtained in less than 2 s at concentrations of 10(-10) mol/liter. To meet the second need, we present two types of data-reduction strategies: (a)a least-squares fit to the data, with use of the spectra of previously determined compounds likely to be present; and (b)a determination of the eigenvalues and eigenvectors fo the fluorescence matrix, from which the number of components and the possible spectra of each can be estimated. Computer simulations of the least-squares fitting algorithms show that five strongly overlapping components can be determined in the presence of noise with an accuracy of better than 5%. Also, a fluorescent sample containing two species with very similar but unknown spectral properties can be resolved to obtain the spectrum of each.

scholarly journals Proteomics: A New Diagnostic Frontier

2006 ◽  
Vol 52 (7) ◽  
pp. 1218-1222 ◽  
Author(s):  
Glen L Hortin ◽  
Saeed A Jortani ◽  
James C Ritchie ◽  
Roland Valdes ◽  
Daniel W Chan
Keyword(s):  
Laboratory Tests ◽  
Clinical Laboratory ◽  
Clinical Chemistry ◽  
Biological Fluids ◽  
Great Promise ◽  
Structural Variations ◽  
Protein Fragments ◽  
Recent Conference ◽  
Complete Set ◽  
Source Of Information

Abstract Background: Analysis of proteins has been an integral part of the field of clinical chemistry for decades. Recent advances in technology and complete identification of the human genome sequence have opened up new opportunities for analysis of proteins for clinical diagnostic purposes. Methods: Content of a recent conference of proteomics is summarized. Results: New analytical methods allow the simultaneous analysis of a large number of proteins in biological fluids such as serum and plasma, offering partial views of the complete set of proteins or proteome. Plasma presents many analytical challenges, such as the complexity of components, predominance of a few major components, and the large concentration range of components, but the number of proteins that can be detected in plasma has expanded dramatically from hundreds to thousands. At the same time, there is increased capability to detect structural variations of proteins. Recent studies also identified the presence of complex sets of small protein fragments in plasma. This set of protein fragments, the fragmentome or peptidome, is potentially a rich source of information about physiologic and disease processes. Conclusions: Advances in proteomics offer great promise for the discovery of markers that might serve as the basis for new clinical laboratory tests. There are many challenges, however, in the translation of newly discovered markers into clinical laboratory tests.

The use of linear models and matrix least squares in clinical chemistry.

1979 ◽  
Vol 25 (6) ◽  
pp. 840-855 ◽  
Author(s):  
S N Deming ◽  
S L Morgan
Keyword(s):  
Least Squares ◽  
Precise Measurement ◽  
Linear Models ◽  
Clinical Chemistry ◽  
Experimental Designs ◽  
Unified Approach

Abstract We present a unified approach to the use of linear models and matrix least squares with the intention of providing a better understanding of the techniques themselves and of the statistics that arise from these techniques as they are used in clinical chemistry. Emphasis is placed on the importance of appropriate experimental designs and adequately precise measurement processes for efficiently obtaining the desired information.

scholarly journals Excitation versus Emission Spectra as a Means to Examine Selective Fluorescence Quenching Agents

1992 ◽  
Vol 46 (9) ◽  
pp. 1388-1392 ◽  
Author(s):  
Sheryl A. Tucker ◽  
William E. Acree
Keyword(s):  
Fluorescence Quenching ◽  
Spectral Data ◽  
Emission Spectra ◽  
Data File ◽  
Excitation Spectra

To ascertain whether fluorescence quenching is best studied with the use of excitation or emission spectra, and to expand our existing PAH spectral data file, we have recorded excitation spectra of benzo[b]perylene, dibenzo[hi,wx]heptacene, tetrabenzo[de,hi,mn,qr]naphthacene, perylene, benzo[a]fluoranthene, benzo[def]indeno[1,2,3hi]chrysene, naphtho[2,1a]fluoranthene, naphtho[2,3b]fluoranthene, benzo[k]fluoranthene, 2-azapyrene, naphtho[8,1,2hij]pyreno[9,10,1def]phthalazine, indeno[1,-2,3ij]isoquinoline, benzo(lmn][3,8]phenanthroline, and 7-methyldibenzo(b,def]chrysene at various nitromethane concentrations. Results of these measurements verify our earlier observations concerning the nitromethane selective quenching rule and further illustrate the importance of considering inner-filtering artifacts in quenching studies.

Synthesis and Characterization of Y2O3:Eu3+ Film Derived by Sol-Gel Processing

2007 ◽  
Vol 336-338 ◽  
pp. 593-596
Author(s):  
Chun Yang ◽  
Jun Ying Zhang ◽  
Hai Bing Feng ◽  
Wei Chang Hao ◽  
Tian Min Wang
Keyword(s):  
Raw Materials ◽  
Sol Gel ◽  
Emission Spectra ◽  
Luminescent Properties ◽  
Dip Coating ◽  
Organic Additive ◽  
Excitation Spectra ◽  
Excitation Peak ◽  
Close Relationship ◽  
Dip Coating Technique

Y2O3:Eu3+ thin film was synthesized by sol-gel method with inorganic salt raw materials, and the crystal structure and luminescent properties were investigated. By adding organic additive to the sol, a homogeneous film with high luminescent intensity could be obtained by dip-coating technique on the surface of alumina sheet and quartz glass. Structures of the films were studied by XRD and SEM. The excitation spectra of the films showed a wide excitation peak from 200nm to 260nm, and the emission spectra had a strongest emission peak at 611nm which revealed a close relationship with the calcining temperature.

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