Simultaneous determination of phosphatidylglycerol and the lecithin/sphingomyelin ratio in amniotic fluid.

1978 ◽  
Vol 24 (7) ◽  
pp. 1144-1146 ◽  
Author(s):  
G R Gotelli ◽  
R E Stanfill ◽  
P M Kabra ◽  
F A Farina ◽  
L J Marton
Keyword(s):  
Thin Layer ◽  
Amniotic Fluid ◽  
Simultaneous Determination ◽  
Chromatographic Method ◽  
White Background ◽  
Molybdenum Blue ◽  
Spray Reagent ◽  
Single Dimension

Abstract We describe a single-dimension thin-layer chromatographic method by which one can simultaneously determine the lecithin/sphingomyelin ratio and the proportion of phosphatidylglycerol in amniotic fluid. The phospholipids are conveniently detected by an improved molybdenum-blue spray reagent, which immediately produces blue spots on a white background. The stained phospholipids are stable for at least 90 min after spraying, and densitometry results in symmetrical peaks that are easily quantitated.

Collaborative Study of a Chromatographic Method for Determination of Patulin in Apple Juice

1974 ◽  
Vol 57 (3) ◽  
pp. 621-625 ◽  
Author(s):  
Peter M Scott
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Column Chromatography ◽  
Apple Juice ◽  
Chromatographic Method ◽  
Collaborative Study ◽  
Spray Reagent ◽  
Contamination Levels ◽  
Layer Chromatography

Abstract Collaborators in 20 laboratories studied a method for the determination of patulin in apple juice. The collaborators mixed patulin solutions with the apple juice to obtain 9 spiked samples (of which 2 were blanks) and 1 practice sample. After column chromatography of the apple juice extract, patulin was estimated visually by thin layer chromatography, using 3-methyl-2-benzothiazolinone hydrazone.HCl as the spray reagent. Mean recoveries of patulin ranged from 69.8 to 98.3% for 3 contamination levels. The method has been adopted as official first action for the determination of patulin in apple juice.

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