High-performance liquid chromatography for ultraviolet-absorbing constituents in human urine.

1979 ◽  
Vol 25 (9) ◽  
pp. 1617-1621 ◽  
Author(s):  
H Miyagi ◽  
J Miura ◽  
Y Takata ◽  
S Ganno
Keyword(s):  
Anion Exchange ◽  
Human Urine ◽  
High Performance ◽  
Exchange Resin ◽  
Gradient Elution ◽  
High Performance Liquid Chromatographic ◽  
Clinical Analysis ◽  
Anion Exchange Resins ◽  
Exchange Resins ◽  
Liquid Chromatographic

Abstract Several liquid-chromatographic systems involving anion-exchange resins and linear acetate gradient elution have been reported previously for the analysis of ultraviolet-absorbing constituents in human urine. We describe a high-performance liquid-chromatographic system on which a macroreticular anion-exchange resin, and stepwise elution are used. Separations were more rapid with ammonium chloride/acetonitrile stepwise elution than with acetate stepwise elution, and urine constituents could be detected at 225 nm with the former system. Analytical conditions were convenient and the method has proven to be useful for routine clinical analysis.

Reversed-Phase High Performance Liquid Chromatographic Analysis of Three First Line Anti-Tuberculosis Drugs

2019 ◽  
Vol 69 (12) ◽  
pp. 3590-3592
Author(s):  
Nela Bibire ◽  
Romeo Iulian Olariu ◽  
Luminita Agoroaei ◽  
Madalina Vieriu ◽  
Alina Diana Panainte ◽  
...  
Keyword(s):  
High Performance ◽  
Biological Fluids ◽  
Gradient Elution ◽  
High Performance Liquid Chromatographic ◽  
Reversed Phase ◽  
Assay Method ◽  
Active Pharmaceutical Ingredients ◽  
First Line ◽  
Pharmaceutical Ingredients ◽  
Liquid Chromatographic

Active pharmaceutical ingredients such as isoniazid, pyrazinamide and rifampicin are among the most important first-line anti-tuberculosis drugs. A simple, rapid and sensitive reversed phase-high performance liquid chromatographic assay method for the simultaneous determination of isoniazid, pyrazinamide and rifampicin has been developed. Separation of the interest compounds was achieved in a 10 min chromatographic run in gradient elution mode on a Zorbax SB-C18 stainless steel column (150 � 4 mm, 5 mm) using a guard column containing the same stationary phase. The gradient elution was carried out with a mobile phase of 10% CH3CN aqueous solution for channel A and 50% CH3CN in pH = 6.8 phosphate buffer (20 mM), to which 1.5 mL triethylamine were added for channel B. Quantification of the analyzed substances was carried out spectrophotometrically at 269 nm. Detection limits of 0.48 mg/L for isoniazid, 0.52 mg/L for pyrazinamide and 0.48 mg/L for rifampicin were established for the developed assay method. The present work showed that the proposed analysis method was advantageous for simple and rapid analysis of the active pharmaceutical ingredients in pharmaceuticals and biological fluids.

Quantitative Analysis of 5-Hydroxymethylfurfural in Linezolid Injection by High Performance Liquid Chromatography

2020 ◽  
Vol 16 (8) ◽  
pp. 1059-1067
Author(s):  
Jéssica Maurício Batista ◽  
Christian Fernandes
Keyword(s):  
High Performance ◽  
Potassium Phosphate ◽  
Gradient Elution ◽  
High Performance Liquid Chromatographic ◽  
Official Method ◽  
Ph Variation ◽  
Drug Products ◽  
Liquid Chromatographic Method ◽  
Liquid Chromatographic

Background: Linezolid is a synthetic broad-spectrum antibacterial belonging to the class of oxazolidinones. Linezolid for intravenous infusion is isotonized with dextrose. In acidic environment, the dehydration of dextrose produces furan derivatives, 5-hydroxymethylfurfural (5-HMF) being the main one. The determination of this degradation product is of fundamental importance, since there is evidence it is cytotoxic, genotoxic, mutagenic and carcinogenic. However, there is no official method for the determination of 5-HMF in drug products. Objective: The aim of this study was to develop and validate a high performance liquid chromatographic method to quantify 5-HMF in injection of linezolid. Methods: The chromatographic separation, after optimization, was performed on C18 (150 x 4.6 mm, 5 μm) column. Mobile phase was composed of 14 mM potassium phosphate buffer pH 3.0 ([H+] = 1.0 x 10-3) and methanol in gradient elution at 1.0 mL min-1. The injection volume was 10 μL and detection was performed at 285 nm. Results: The method was optimized and validated, showing selectivity, linearity in the range from 0.075 to 9.0 μg mL-1, precision (RSD ≤ 2.0%), accuracy (mean recovery of 100.07%) and robustness for temperature and pH variation. Conclusion: The method was shown to be adequate to determine 5-HMF in injection containing linezolid in routine analysis.

scholarly journals Mg/Al double-metal hydroxide regeneration of anion exchange resin by electric field intensification

2016 ◽  
Vol 75 (6) ◽  
pp. 1309-1318 ◽  
Author(s):  
Ying Wang ◽  
Zhun Li ◽  
Yansheng Li ◽  
Zhigang Liu
Keyword(s):  
Electric Field ◽  
Anion Exchange ◽  
Exchange Resin ◽  
Ph Value ◽  
Anion Exchange Resin ◽  
Cost Effective ◽  
Regenerative Capacity ◽  
Metal Hydroxides ◽  
Anion Exchange Resins ◽  
Exchange Resins

Fouled anion exchange resins were regenerated by electric field intensification of Mg/Al double-metal hydroxides. Regenerative experiments were performed with varying voltages (10–30 V) and dosages of Mg/Al hydroxides (0.045–0.135 mol and 0.015–0.045 mol, respectively) for 1–5 h. Optimal results were obtained under the following regenerative conditions: 20 V, 4 h, and 0.09/0.03 mol of Mg/Al hydroxides. The maximum regenerative capacity of resins was increased to 41.07%. The regenerative mechanism was presented by Fourier-transform infrared spectrum of resins and Mg/Al hydroxides, and the regenerative degree was analyzed with respect to conductivity, pH value, and electric current. Mg/Al hydroxides were also recycled after the regeneration. This method was proven to be cost-effective and environmentally friendly.

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