Radioimmunoassay of osteocalcin with polyclonal and monoclonal antibodies.

1989 ◽  
Vol 35 (7) ◽  
pp. 1408-1415 ◽  
Author(s):  
M J Power ◽  
J P Gosling ◽  
P F Fottrell
Keyword(s):  
Monoclonal Antibodies ◽  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Normal Range ◽  
Serum Samples ◽  
Wide Range ◽  
Assay Precision

Abstract We have developed and thoroughly validated radioimmunoassays (RIAs) for osteocalcin in plasma and serum, demonstrating independence of volume and determining the recovery of added standard and within- and between-assay precision. Antibodies were raised in rabbits and mice by immunization with either osteocalcin adsorbed to polyvinylpyrrolidone or osteocalcin conjugated to bovine serum albumin. In both species, use of the conjugated osteocalcin was more efficacious. Correlation of the results obtained when groups of plasma or serum samples were analyzed by RIAs with different antibodies (two polyclonal and one monoclonal) indicated that the normal range observed for osteocalcin concentrations in serum depends on the antibody used. These findings may account for the wide range of "normal" osteocalcin values reported by different groups.

Monoclonal antibodies to bovine serum albumin: Affinity and specificity determinations

1988 ◽  
Vol 25 (1) ◽  
pp. 7-15 ◽  
Author(s):  
Guillemette A. Morel ◽  
David M. Yarmush ◽  
Clark K. Colton ◽  
David C. Benjamin ◽  
Martin L. Yarmush
Keyword(s):  
Monoclonal Antibodies ◽  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum

scholarly journals Heterologous screening of hybridomas for the development of broad-specific monoclonal antibodies against deoxynivalenol and its analogues

2014 ◽  
Vol 7 (3) ◽  
pp. 257-265 ◽  
Author(s):  
Y. Guo ◽  
M. Sanders ◽  
A. Galvita ◽  
A. Heyerick ◽  
D. Deforce ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Bovine Serum Albumin ◽  
Horseradish Peroxidase ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Simultaneous Detection ◽  
Cross Reactivity ◽  
Efficient Approach ◽  
Selection For ◽  
Similar Affinity

Hapten heterology was introduced into the steps of hybridoma selection for the development of monoclonal antibodies (MAbs) against deoxynivalenol (DON). Firstly, a novel heterologous DON hapten was synthesised and covalently coupled to proteins (i.e. bovine serum albumin (BSA), ovalbumin and horseradish peroxidase) using the linkage of cyanuric chloride (CC). After immunisation, antisera from different DON immunogens were checked for the presence of useful antibodies. Next, both homologous and heterologous enzyme-linked immunosorbent assays were conducted to screen for hybridomas. It was found that heterologous screening could significantly reduce the proportion of false positives and appeared to be an efficient approach for selecting hybridomas of interest. This strategy resulted in two kinds of broad-selective MAbs against DON and its analogues. They were quite distinct from other reported DON-antibodies in their cross-reactivity profiles. A unique MAb 13H1 derived from DON-CC-BSA immunogen could recognise DON and its analogues in the order of HT-2 toxin ≯ 15-acetyl-DON ≯ DON ≯ nivalenol, with IC50 ranging from 1.14 to 7.69 μg/ml. Another preferable MAb 10H10 generated from DON-BSA immunogen manifested relatively similar affinity to DON, 3-acetyl-DON and 15-acetyl-DON, with IC50 values of 22, 15 and 34 ng/ml, respectively. This is the first broad-specific MAb against DON and its two acetylated forms and thus it can be used for simultaneous detection of the three mycotoxins.

scholarly journals Bovine Serum Albumin Antibodies as a Disease Marker for Hepatitis E Virus Infection

2005 ◽  
Vol 2005 (4) ◽  
pp. 316-321 ◽  
Author(s):  
Medhat Haroun
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Acute Hepatitis ◽  
Bovine Serum ◽  
Hepatitis E ◽  
Serum Samples ◽  
Disease Marker ◽  
Normal Individuals ◽  
Significant Difference ◽  
Acute Hepatitis E

This report evaluates the significance of antibody/bovine serum albumin (BSA) interactions as a risk factor for the diagnosis of acute hepatitis E. Serum samples from 40 patients with acute hepatitis E and from 40 age/sex matched healthy adult subjects were tested for IgA, IgG, and IgM by ELISA and by turbidimetric assay. BSA was used as a target to characterize changes in levels of interacting immunoglobulins. Initial results obtained before removal of antibodies that interacted with BSA suggested that HEV patients had increased levels of IgM in their sera. It was found that normal individuals had mean IgA, IgG, and IgM levels of2.55mg/mL,9.80mg/mL, and1.73mg/mL, respectively while HEV patients had mean levels of2.66mg/mL,10.04mg/mL, and2.01mg/mL (P<.26,P<.32, andP<.0004). However, the mean level of IgM in HEV-infected sera after purification from antibodies that interacted with BSA was determined to be1.72mg/mL indicating that there was no significant difference in IgM level in HEV patients compared to normal individuals (P<.6). The presence of antibodies that interact with BSA might serve as a diagnostic tool for detection of high-risk patients.

Human corticotropin (ACTH) radioimmunoassay with synthetic 1--24 ACTH.

1979 ◽  
Vol 25 (7) ◽  
pp. 1267-1273 ◽  
Author(s):  
P C Kao ◽  
N S Jiang ◽  
P C Carpenter
Keyword(s):  
Polyethylene Glycol ◽  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Confidence Limit ◽  
Normal Subjects ◽  
Cross Reactivity ◽  
Normal Range ◽  
Beta Endorphin

Abstract A corticotropin antiserum was obtained from rabbits immunized with synthetic 1--24 corticotropin conjugated with bovine serum albumin. The antiserum did not cross react with synthetic alpha-melanotropin or with synthetic beta-endorphin and had a cross reactivity of 0.23% with human beta-lipotropin. We developed a radioimmunoassay with the antiserum obtained, in which we used polyethylene glycol in conjunction with a second precipitating antibody for fast (15-min) separation of antibody-bound and free corticotropin. The assay had a sensitivity of 16 ng/L and was validated on patients with various pituitary and adrenal diseases. From 103 normal subjects, the median value for corticotropin in specimens collected during the morning was 34 ng/L of plasma; the upper 95% confidence limit of the normal range was 98 ng/L.

Whole-body measurement of radioactivity as a means of following in vivo the degradation of I131-labeled proteins in mice

1960 ◽  
Vol 198 (6) ◽  
pp. 1355-1360 ◽  
Author(s):  
Geronimo Terres ◽  
W. L. Hughes ◽  
W. Wolins
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Trichloroacetic Acid ◽  
Whole Body ◽  
Body Measurements ◽  
Body Measurement ◽  
Serum Samples ◽  
Secular Equilibrium

Mice were injected with I131-labeled human or bovine serum albumin and determinations made of the rate of loss of radioactivity a) from the whole body ( in vivo counting), b) from the blood (serum samples), and c) from the total trichloroacetic acid precipitable mouse proteins (following homogenization). Measurements by each method made more than 8 hours post-injection gave the same half life (14.5 ± 0.5 hr.) for I131 bovine serum albumin (I131 BSA) when the level of circulating iodide was sufficient to prevent thyroidal accumulation (I131 HSA had a half time of 21 hr.). The variations observed between the methods in the first 24 hours can be explained in terms of times required for the several compartments to reach secular equilibrium, and therefore whole-body measurements in vivo can be safely used to measure the rate of degradation of this protein when allowance for these factors is made.

Immunoregulation of the anti-bovine serum albumin response by polyclonal and monoclonal antibodies

1983 ◽  
Vol 80 (2) ◽  
pp. 279-287 ◽  
Author(s):  
Nancy J. Krieger ◽  
Amadeo Pesce ◽  
J.Gabriel Michael
Keyword(s):  
Monoclonal Antibodies ◽  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum
Sign in / Sign up

Export Citation Format

Share Document