Effects of serum-isolated vs synthetic bilirubin-albumin complexes on dye-binding methods for estimating serum albumin

1991 ◽  
Vol 37 (7) ◽  
pp. 1269-1272 ◽  
Author(s):  
H Ihara ◽  
H Nakamura ◽  
Y Aoki ◽  
T Aoki ◽  
M Yoshida
Keyword(s):  
Serum Albumin ◽  
Molar Ratio ◽  
Green Method ◽  
Dye Binding ◽  
Bromcresol Green

Abstract Bush and Reed reported (Clin Chem 1987;33:821-3) that the reaction of albumin with bromcresol purple but not with bromcresol green underestimated the concentration of albumin in synthetically obtained bilirubin-albumin (Bd) by 29%. Their unproven assumption was that chemically synthesized Bd behaved in a manner indistinguishable from the natural Bd in icteric serum. Here we verify that Bd, whether synthetically obtained or isolated from serum, causes an underestimation of albumin in the bromcresol purple but not in the bromcresol green method. The molar ratio of Bd from either source to underestimated albumin approximates 1.0, suggesting that one molecule of Bd would react equivalently to a molecule of albumin in the bromcresol purple method. This underestimation might falsely suggest hypoalbuminemia in patients with increased serum Bd.

Comparison of several methods for semiquantitative determination of urinary protein.

1977 ◽  
Vol 23 (5) ◽  
pp. 876-879 ◽  
Author(s):  
W L Gyure
Keyword(s):  
Serum Albumin ◽  
Salt Concentration ◽  
Urine Samples ◽  
Urine Protein ◽  
Sulfosalicylic Acid ◽  
Green Method ◽  
Semiquantitative Determination ◽  
Acid Method ◽  
Bromcresol Green

Abstract Two types of urine protein dipsticks and the sulfosalicylic acid method were compared for their accuracy and specificity, with use of urine samples supplemented with various proteins. Dipsticks yield accurate results when the protein under consideration is restricted to albumin; the sulfosalicylic acid method accurately determines many kinds of proteins in addition to albumin. Detergents affect each of the methods, but changes in salt concentration only affect results by dipstick procedures. Dipsticks, which are based on the protein-error principle for indicators, are subject to some of the conditions that apply to the bromcresol green method for serum albumin determination.

Improved continuous-flow (SMAC) determination of serum albumin.

1978 ◽  
Vol 24 (7) ◽  
pp. 1191-1193 ◽  
Author(s):  
G Cederblad ◽  
B E Hickey ◽  
A Hollender ◽  
G Akerlund
Keyword(s):  
Reaction Time ◽  
Serum Albumin ◽  
Acute Phase ◽  
Continuous Flow ◽  
Green Method ◽  
Acute Phase Reactants ◽  
Modified Method ◽  
Continuous Flow Method ◽  
Bromcresol Green

Abstract The albumin values determined by the bromcresol green methods do not compare well with values by more specific methods for albumin determination. The discrepancies have been related to, among other things, acute-phase reactants and are especially pronounced in the lower albumin range. These disadvantages are also inherent in a routine continuous-flow method for albumin (SMAC). The bromcresol green method has been improved considerably by shortening the reaction time before the absorbance is measured, as is described here. The modified method yields values that better agree with those by more specific methods and an influence of acute-phase reactants is no longer observed.

Influence of Reagent Quality and Reaction Conditions on the Determination of Serum Albumin by the Bromcresol Green Dye-Binding Method

1977 ◽  
Vol 14 (1-6) ◽  
pp. 105-115 ◽  
Author(s):  
K. Spencer ◽  
C. P. Price
Keyword(s):  
Quality Control ◽  
Serum Albumin ◽  
Interlaboratory Comparison ◽  
Reaction Conditions ◽  
Control Techniques ◽  
Dye Binding ◽  
Quality Control Techniques ◽  
Bromcresol Green

In an age of reference methodologies and sophisticated quality control techniques it is surprising that so little attention is paid to the quality of the reagents that are used. This paper reports on an investigation of the bromcresol green dye-binding procedure for the estimation of serum albumin in which particular attention has been paid to changes in the reagent components that contribute to the performance of the method. It has been shown that detailed attention to the quality control of the reagents can improve the precision and interlaboratory comparison in the estimation of serum albumin.

Determination of Serum Albumin with the "SMA 12/60" by a Bromcresol Green Dye-Binding Method

1972 ◽  
Vol 18 (7) ◽  
pp. 647-653 ◽  
Author(s):  
James O Westgard ◽  
Michael A Poquette
Keyword(s):  
Quality Control ◽  
Serum Albumin ◽  
Cellulose Acetate ◽  
Normal Range ◽  
Plasma Hemoglobin ◽  
Routine Service ◽  
Brij 35 ◽  
Dye Binding ◽  
Bromcresol Green

Abstract A bromcresol green (BCG) method for serum albumin has been adapted to the Technicon "SMA 12/60." Sensitivity and linearity critically depend on the concentrations of BCG and surfactant ("Brij-35"). With appropriate concentrations, linearity to 6.0 g/dl is achieved. Interaction is 2-3%. Standard deviations for quality control pools of 2.0 and 4.7 g/dl during three months of routine service were 0.06 and 0.11 g/dl. Values agree well with values determined by the Du Pont "ACA" (bias = 0.047 g/dl, SD = 0.191 g/dl, t = 1.746, r = 0.975, n = 53), by a modified Beckman "DSA" method in which BCG is also used (bias = 0.006, SD = 0.164, t = 0.398, r = 0.976, n = 115), and by electrophoresis on cellulose acetate (bias = 0.028, SD = 0.203, t = 1.006, r = 0.961, n = 54). Blank errors for hemolyzed samples (plasma hemoglobin up to 350 mg/dl), icteric samples (bilirubin up to 30 mg/dl), and lipemic samples (triglycerides up to 500 mg/dl) are 0.2 g/dl or less when the blank channel is not used. Normal range is estimated to be 3.5 to 4.8 g/dl (n = 145; 2.5 and 97.5 percentiles).

Albumin Quantitation by Dye Binding and Salt Fractionation Techniques

1972 ◽  
Vol 18 (1) ◽  
pp. 52-56 ◽  
Author(s):  
Don S Miyada ◽  
Vernon Baysinger ◽  
Solomon Notrica ◽  
R M Nakamura
Keyword(s):  
Linear Regression ◽  
Benzoic Acid ◽  
Serum Albumin ◽  
Correlation Coefficients ◽  
Regression Equations ◽  
Comparison Of Results ◽  
Dye Binding ◽  
Colorimetric Procedure ◽  
Bromcresol Green ◽  
Salt Fractionation

Abstract Two dye-binding reagents, 2-(4'-hydroxybenzeneazo)benzoic acid (HABA) and bromcresol green (BCG), for the quantitation of serum albumin have been compared with the biuret colorimetric procedure after salt fractionation. The linear regression equations for the biuret-BCG and the biuret-HABA comparisons were y = 0.43 + 0.91x and y = -0.45 + 1.15x, respectively. Their corresponding correlation coefficients were 0.93 and 0.83, respectively. The effects of icterus, lipemia, and hemolysis on the dye-binding procedures were generally greater in the case of the HABA procedure. A comparison of results with BCG and HABA for 20 sera having A/G ratios of less than 1, indicates them to be equally reliable.

Serum albumin estimation: Modification of the bromcresol green method

1972 ◽  
Vol 37 ◽  
pp. 117-121 ◽  
Author(s):  
I.G. McPherson ◽  
D.W. Everard
Keyword(s):  
Serum Albumin ◽  
Green Method ◽  
Bromcresol Green
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