Glutamine stability in biological tissues evaluated by fluorometric analysis

1993 ◽  
Vol 39 (6) ◽  
pp. 1059-1063 ◽  
Author(s):  
V B Grossie ◽  
J Yick ◽  
M Alpeter ◽  
T C Welbourne ◽  
D M Ota
Keyword(s):  
Aqueous Solution ◽  
Amino Acid ◽  
High Efficiency ◽  
Biological Tissues ◽  
Measured Concentration ◽  
Important Amino Acid ◽  
Tissue Extracts ◽  
Normal Rat ◽  
Fluorometric Analysis ◽  
The Stability

Abstract Although glutamine has been considered unstable during storage and therefore difficult to quantitate, recent results suggest this amino acid is stable at low pH ranges. We evaluated the stability of glutamine in plasma and tissue extracts, using fluorometric analysis. The measured concentration of glutamine detected varied linearly up to 0.8 mmol/L for the aqueous solution (r2 = 98.7, P = 0.0001) with a mean (+/- SD) coefficient of variation of 2.41% +/- 0.79%. When glutamine was dissolved in 50 g/L trichloroacetic acid (TCA), the values were essentially unaltered. Glutamine in an aqueous solution and stored at -70 degrees C was stable for at least 16 days; glutamine in TCA was stable for 6-8 days, then decreased to a concentration significantly lower than that of the aqueous solution. The expected and observed concentrations in plasma were equal (r2 = 0.99975) for increasing amounts of added glutamine. Glutamine concentrations in plasma were stable for > 1 year when stored at -70 degrees C. The glutamine of a transplantable rat sarcoma and a normal rat liver could be extracted with 50 g/L TCA with high efficiency (88.6% +/- 1.9% and 90.2% +/- 0.04%, respectively); the extracted glutamine is stable in TCA for at least 7 days without neutralization when stored at -70 degrees C. Fluorometric analysis of glutamine required only a small quantity of plasma (25 microL) or tissue (200 mg) and is a convenient method for quantifying this important amino acid.

REMOVAL OF HEAVY METAL Cr (II), Ni (II), Cu (II), Zn (II), Cd (II), Pb (II) IONS FROM AQUEOUS SOLUTION BY MENTHA PIPERITA EXTRACT

2020 ◽  
pp. 15-20
Author(s):  
Ersin Yucel ◽  
Mine Yucel
Keyword(s):  
Aqueous Solution ◽  
Metal Ions ◽  
High Efficiency ◽  
Correlation Coefficients ◽  
Langmuir Model ◽  
Mentha Piperita ◽  
Freundlich Model ◽  
Biosorption Capacity ◽  
Peppermint Extract ◽  
The Stability

In this study, the usage of the peppermint (Mentha piperita) for extracting the metal ions [Mg (II), Cr (II), Ni (II), Cu (II), Zn (II), Cd (II), Pb (II)] that exist at water was investigated. In order to analyze the stability properties, Langmuir, Freundlich, Temkin and Dubinin-Radushkevich isotherms were used at removing the metal ions and the highest correlation coefficients (R2) were obtained at Langmuir isotherm. Therefore, it is seen that the Langmuir model is more proper than the Freundlich model. However, it was found that the correlation coefficients of removing Ni and Cd is higher at Freundlich model than Langmuir and low at Dubinin-Radushkevich isotherm. It is established that the biosorption amount increase depends on the increase of biosorbent and it can be achieved high efficiency (95%) even with small amount (0.6 mg, peppermint extract) at lead ions. It is also determined that the peppermint extracted that is used at this study shows high biosorption capacity for metal ions and can be used for immobilization of metals from polluted areas.

The Stability and Structure of Complex Species Formed in Equilibrium Reactions of Diethyltin(IV) with N-D-Gluconylamino Acids in Aqueous Solution

1995 ◽  
Vol 50 (4) ◽  
pp. 515-523 ◽  
Author(s):  
B. Gyurcsik ◽  
N. Buzás ◽  
T. Gajda ◽  
L. Nagy ◽  
E. Kuzmann ◽  
...  
Keyword(s):  
Aqueous Solution ◽  
Amino Acid ◽  
Water Soluble ◽  
Complex Formation Equilibria ◽  
Complex Species ◽  
Carboxylate Oxygen ◽  
Equilibrium Reactions ◽  
Formation Equilibria ◽  
The Stability ◽  
Ph Range

Complex formation equilibria of diethyltin(IV) with five N-D-gluconylamino acids in aqueous solution (I = 0.1 M, NaClO4) were studied and the stabilities of the species were determined by potentiometric titrations. Diethyltin(IV) complexes of α-amino acid derivatives are water-soluble in the physiological pH range, while in the presence of N-D-gluconyl-β-alanine a precipitate is formed, which dissolves with increasing pH. 13C NMR measurements showed that in the N-D-gluconyl-α-amino acid complexes the ligand is coordinated through its deprotonated carboxylate oxygen, amide nitrogen and C(2)-hydroxy group, while for the soluble N-D-gluconyl-β-alanine complex the ligand is coordinated via the deprotonated carboxylate and C(3)-, C(4)-, C(5)-hydroxy groups. Mössbauer measurements reflected the geometry of the complexes formed.

Use of N-Carboxyanhydrides in the Synthesis of Dipeptides. Factors Affecting the Yields of Several Alanine and Valine-containing Dipeptides

1973 ◽  
Vol 51 (8) ◽  
pp. 1284-1287 ◽  
Author(s):  
Eric M. Grovestine ◽  
Jean R. Langlois ◽  
Ross E. Williams
Keyword(s):  
Aqueous Solution ◽  
Amino Acid ◽  
Side Chains ◽  
Organic Base ◽  
Factors Affecting ◽  
The Stability

A series of dipeptides having aliphatic side chains have been prepared in aqueous solution from alanine and valine NCA. An organic base was used to establish and maintain the pH. After the reaction the amounts of unreacted amino acid nucleophile and the overall yields in the reaction were determined. Our observations have led us to propose that NCA stability, the pKa of the amino acid nucleophile and the stability of the carbamate all can play an important role in determining synthetic yields during these preparations.

Aqueous solution thickening of amino acid‐based surfactant by alkylpyrrolidone

2021 ◽  
Author(s):  
Xuemin Liu ◽  
Ke Wu ◽  
Weili Song ◽  
Qiuyun Lei ◽  
Hui Zhang ◽  
...  
Keyword(s):  
Aqueous Solution ◽  
Amino Acid

scholarly journals STUDIES ON THE FUNCTION OF INTRACELLULAR RIBONUCLEASES

1966 ◽  
Vol 29 (3) ◽  
pp. 395-403 ◽  
Author(s):  
Takeshi Utsunomiya ◽  
Jay S. Roth
Keyword(s):  
Natural Products ◽  
Sodium Chloride ◽  
Rat Liver ◽  
Messenger Rna ◽  
Rnase Activity ◽  
Optimum Ph ◽  
Normal Rat ◽  
Pancreatic Rnase ◽  
The Stability ◽  
Normal Constituent

The RNase activity and properties of ribosome and polysome preparations from normal rat liver and some hepatomas have been examined. Polysome and ribosome preparations from the Novikoff, McCoy MDAB, and Dunning hepatomas had considerably higher specific RNase activity than corresponding preparations from normal rat liver, Novikoff ascites, or Morris 5123 hepatomas. The optimum pH of the RNase was approximately 8.5 for all samples tested, and the samples showed no evidence of latent RNase activity when treated with 3 M sodium chloride, EDTA, urea, or p-chloromercuribenzenesulfonic acid. The RNase activity appeared to be associated principally with breakdown products and/or subunits smaller than 80S. In the presence of Mg++ ions, subunits could reaggregate to form monomer ribosomes indistinguishable from the natural products, but some of the reassociated ribosomes could contain RNase activity which had been bound to the smaller particles. Similar results were obtained with spermine. In the hepatomas, evidence was obtained for the preexistence of considerable amounts of the smaller, RNase-containing subunits in the cell. When a small amount of crystalline bovine pancreatic RNase was added to partly dissociated ribosomes, the RNase was found only in association with the smaller subunits, and little or no enzyme was taken up by ribosomes or polysomes. The results have led to the conclusion that RNase is not a normal constituent of the ribosome or polysome, but that RNase may become associated with these particulates if dissociation and reassociation take place. Some implications of these findings for the stability of messenger RNA and for the mechanism of its breakdown are discussed.

Cationic poly-L-amino acid-enhanced selective hydrogen production based on formate decomposition with platinum nanoparticles dispersed by polyvinylpyrrolidone

2021 ◽  
Author(s):  
Yusuke Minami ◽  
Yutaka Amao
Keyword(s):  
Aqueous Solution ◽  
Amino Acid ◽  
Hydrogen Production ◽  
Formic Acid ◽  
Platinum Nanoparticles ◽  
Hydrogen Carrier ◽  
Low Toxicity ◽  
Formate Decomposition

Formate is attracting attention as a hydrogen carrier because of its low toxicity and easy handling in aqueous solution. In order to utilize formic acid as a hydrogen carrier, a...

scholarly journals High efficiency removal of Pb(ii) in aqueous solution by a biochar-supported nanoscale ferrous sulfide composite

RSC Advances ◽  
2021 ◽  
Vol 11 (2) ◽  
pp. 953-959
Author(s):  
Chengguang Chen ◽  
Muqing Qiu
Keyword(s):  
Aqueous Solution ◽  
High Efficiency ◽  
Ferrous Sulfide

A biochar-supported nanoscale ferrous sulfide composite was prepared and applied for the treatment of Pb(ii) ions in aqueous solution.

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