scholarly journals Diagnostic value of various serum antibodies detected by diverse methods in childhood celiac disease

1996 ◽  
Vol 42 (11) ◽  
pp. 1838-1842 ◽  
Author(s):  
L Sacchetti ◽  
A Ferrajolo ◽  
G Salerno ◽  
P Esposito ◽  
M M Lofrano ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Celiac Disease ◽  
Umbilical Cord ◽  
Diagnostic Value ◽  
Diagnostic Sensitivity ◽  
Antibody Detection ◽  
Human Umbilical Cord ◽  
Iga Antibody ◽  
Monkey Esophagus ◽  
Gliadin Antibody

Abstract The diagnostic performances of antiendomysium IgA detected on monkey esophagus and human umbilical cord smooth muscle, of antireticulin IgA, and of antigliadin IgA and IgG were calculated in 74 children with celiac disease (CD) or other gastrointestinal disorders. We also compared four methods for gliadin antibody detection. With a diagnostic specificity of 100%, diagnostic sensitivity was 94% for antireticulin IgA, 93% for antiendomysium IgA when detected on human umbilical cord smooth muscle, and 97% when detected on monkey esophagus. The diagnostic sensitivity for gliadin antibody was highest with an ELISA procedure, followed by fluorogenic detection (94% for IgG, 91% for IgA, 97% with IgA and IgG combined). Because of its high diagnostic sensitivity and ease and speed of use, the combined antigliadin IgG and IgA antibody assay is suitable for screening large groups of patients. In IgG- or IgA-positive cases, the more demanding and more specific antiendomysium IgA evaluation is required to confirm suspected CD.

scholarly journals IgG Antibodies against Deamidated Gliadin Peptides for Diagnosis of Celiac Disease in Patients with IgA Deficiency

2010 ◽  
Vol 56 (3) ◽  
pp. 464-468 ◽  
Author(s):  
Danilo Villalta ◽  
Elio Tonutti ◽  
Christian Prause ◽  
Sibylle Koletzko ◽  
H Holm Uhlig ◽  
...  
Keyword(s):  
Celiac Disease ◽  
Blood Donors ◽  
Tissue Transglutaminase ◽  
Iga Deficiency ◽  
Diagnostic Sensitivity ◽  
Igg Antibodies ◽  
Diagnostic Specificity ◽  
Gliadin Peptides ◽  
Endomysium Antibodies ◽  
Monkey Esophagus

AbstractBackground: Assays for IgG antibodies against deamidated gliadin (IgG-anti-dGli) are comparable in performance with tests detecting IgA antibodies against tissue transglutaminase (IgA-anti-tTG) in diagnosing celiac disease (CD). IgA-anti-tTG are absent in IgA deficiency, a condition often associated with CD. In IgA deficiency, IgG-anti-tTG, which have a lower overall diagnostic accuracy, are routinely measured. We examined whether IgG-anti-dGli would be useful for diagnosing CD in patients with IgA deficiency.Methods: We studied 34 IgA-deficient CD patients, 185 IgA-competent newly diagnosed children with CD, 316 children without CD, 400 adult blood donors, and 6 control IgA-deficient individuals without CD. Anti-dGli and anti-tTG were measured by ELISA, and endomysium antibodies (EmA) were measured by immunofluorescence on monkey esophagus (IgA as well as IgG class for all antibodies). We calculated diagnostic sensitivity (percentage of patients above cutoff with 95% CIs) according to age-specific cutoffs for 95% diagnostic specificity and according to cutoffs proposed by the manufacturer of the assays.Results: No IgA-deficient CD patients were positive for any IgA-based antibody assay. Diagnostic sensitivity of IgG-anti-tTG was 91.2% (95% CI 76.3%–97.7%) according to age-specific cutoffs and 82.4% (66.1%–92.0%) according to manufacturer cutoffs. The diagnostic sensitivity of IgG-EmA was 75.8% (58.8%–87.4%) and the sensitivity of IgG-anti-dGli was 88.2% (72.8%–95.9%) according to both cutoffs.Conclusions: IgG-anti-dGli and IgG-anti-tTG have comparable diagnostic sensitivities for IgA-deficient celiac patients. IgG-anti-dGli may be useful for diagnosing CD in IgA-deficient patients.

scholarly journals The importance of anti-transglutaminase IgA antibody detection in the diagnosis of celiac disease – case report of an inappropriate diagnostic approach

2015 ◽  
Vol 4 ◽  
pp. 250-253
Author(s):  
Edyta Szymańska ◽  
Sylwia Szymańska ◽  
Joanna Pawłowska ◽  
Ewa Orłowska ◽  
Ewa Konopka ◽  
...  
Keyword(s):  
Celiac Disease ◽  
Case Report ◽  
Diagnostic Approach ◽  
Antibody Detection ◽  
Disease Case ◽  
Iga Antibody

scholarly journals Birth weight and characteristics of endothelial and smooth muscle cell cultures from human umbilical cord vessels

2009 ◽  
Vol 7 (1) ◽  
pp. 30 ◽  
Author(s):  
José Javier Martín de Llano ◽  
Graciela Fuertes ◽  
Isabel Torró ◽  
Consuelo García Vicent ◽  
José Luis Fayos ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Birth Weight ◽  
Smooth Muscle Cell ◽  
Umbilical Cord ◽  
Muscle Cell ◽  
Cell Cultures ◽  
Human Umbilical Cord ◽  
Smooth Muscle Cell Cultures

Expression Analysis of the SG-SSPN Complex in Smooth Muscle and Endothelial Cells of Human Umbilical Cord Vessels

2005 ◽  
Vol 42 (1) ◽  
pp. 1-7 ◽  
Author(s):  
I. Ramírez-Sánchez ◽  
H. Rosas-Vargas ◽  
G. Ceballos-Reyes ◽  
F. Salamanca ◽  
R.M. Coral-Vázquez
Keyword(s):  
Endothelial Cells ◽  
Smooth Muscle ◽  
Umbilical Cord ◽  
Expression Analysis ◽  
Human Umbilical Cord

Effects of activated eosinophils cultured from human umbilical cord blood on guinea pig trachealis

1993 ◽  
Vol 265 (3) ◽  
pp. L301-L307 ◽  
Author(s):  
K. J. Hamann ◽  
M. E. Strek ◽  
S. L. Baranowski ◽  
N. M. Munoz ◽  
F. S. Williams ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Guinea Pig ◽  
Cord Blood ◽  
Umbilical Cord ◽  
Umbilical Cord Blood ◽  
Human Peripheral Blood ◽  
Human Umbilical Cord Blood ◽  
Human Umbilical Cord ◽  
Lipoxygenase Inhibitor

We studied the biochemical indexes and corresponding induction of airway smooth muscle contraction and hyperresponsiveness in guinea pig trachealis in situ caused by cultured eosinophils derived from mononuclear cell fractions of human umbilical cord blood. A method was developed that permitted isolation of large numbers of cells (approximately 2.6 x 10(6)/ml cord blood) having morphological and immunohistological characteristics of human peripheral blood eosinophils. After activation with 10(-6) M formyl-Met-Leu-Phe + 5 micrograms/ml cytochalasin B (fMLP + B), in situ application to the epithelial surface of 6 x 10(6) cord-derived eosinophils (CDE)/surface area (cm2) caused 1.46 +/- 0.24 g/cm maximal active tracheal tension in guinea pig tracheal smooth muscle (P < 0.005 vs. zero baseline). Muscarinic responsiveness also was augmented in situ in trachealis preparations treated with activated 3-wk CDE. Contraction caused by 3 x 10(-7) mol/kg iv methacholine (MCh) was 0.94 +/- 0.18 g/cm at baseline vs. 1.80 +/- 0.24 g/cm after activated CDE (P = 0.02). Control (sham-activated) 3-wk CDE caused neither significant contraction [0.41 +/- 0.16 g/cm active tension (AT); P < 0.05 vs. fMLP+B] nor augmented muscarinic responsiveness. Cells cultured for 5 wk contained fewer granules than 3-wk CDE and also caused less direct contraction of trachealis (0.73 +/- 0.14 g/cm AT) after activation (P < 0.01 vs. 3-wk CDE). Both contraction and muscarinic augmentation were blocked in 3-wk CDE after blockade of leukotriene C4 (LTC4) synthesis by pretreatment with the 5-lipoxygenase inhibitor, A63162 (50 microM). Treatment with A63162 had no effect on the stimulated release of eosinophil peroxidase.(ABSTRACT TRUNCATED AT 250 WORDS)

IgA anti-endomysial antibodies on human umbilical cord tissue for celiac disease screening

1995 ◽  
Vol 40 (9) ◽  
pp. 1902-1905 ◽  
Author(s):  
Umberto Volta ◽  
Nicolino Molinaro ◽  
Lucia De Franceschi ◽  
Domenico Fratangelo ◽  
Francesco Bianco Bianchi
Keyword(s):  
Celiac Disease ◽  
Umbilical Cord ◽  
Disease Screening ◽  
Human Umbilical Cord ◽  
Umbilical Cord Tissue ◽  
Endomysial Antibodies
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