Determining IgA and IgG Antigliadin, IgA Antitransglutaminase, and Antiendomysial Antibodies in Monkey Esophagus and in Umbilical Cord for Diagnosis of Celiac Disease in Developing Countries

2007 ◽  
Vol 45 (5) ◽  
pp. 551-558 ◽  
Author(s):  
Magda Bahia ◽  
Francisco José Penna ◽  
Ivan Barbosa Machado Sampaio ◽  
Geraldo Magela Fritz Silva ◽  
Eugênio Marcos Goulart Andrade
Keyword(s):  
Developing Countries ◽  
Celiac Disease ◽  
Umbilical Cord ◽  
Monkey Esophagus ◽  
Antiendomysial Antibodies

scholarly journals Diagnostic value of various serum antibodies detected by diverse methods in childhood celiac disease

1996 ◽  
Vol 42 (11) ◽  
pp. 1838-1842 ◽  
Author(s):  
L Sacchetti ◽  
A Ferrajolo ◽  
G Salerno ◽  
P Esposito ◽  
M M Lofrano ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Celiac Disease ◽  
Umbilical Cord ◽  
Diagnostic Value ◽  
Diagnostic Sensitivity ◽  
Antibody Detection ◽  
Human Umbilical Cord ◽  
Iga Antibody ◽  
Monkey Esophagus ◽  
Gliadin Antibody

Abstract The diagnostic performances of antiendomysium IgA detected on monkey esophagus and human umbilical cord smooth muscle, of antireticulin IgA, and of antigliadin IgA and IgG were calculated in 74 children with celiac disease (CD) or other gastrointestinal disorders. We also compared four methods for gliadin antibody detection. With a diagnostic specificity of 100%, diagnostic sensitivity was 94% for antireticulin IgA, 93% for antiendomysium IgA when detected on human umbilical cord smooth muscle, and 97% when detected on monkey esophagus. The diagnostic sensitivity for gliadin antibody was highest with an ELISA procedure, followed by fluorogenic detection (94% for IgG, 91% for IgA, 97% with IgA and IgG combined). Because of its high diagnostic sensitivity and ease and speed of use, the combined antigliadin IgG and IgA antibody assay is suitable for screening large groups of patients. In IgG- or IgA-positive cases, the more demanding and more specific antiendomysium IgA evaluation is required to confirm suspected CD.

scholarly journals Changes in Age at Diagnosis and Nutritional Course of Celiac Disease in the Last Two Decades

Nutrients ◽  
2020 ◽  
Vol 12 (1) ◽  
pp. 156 ◽  
Author(s):  
Mónica Villanueva ◽  
Amaya Oyarzún ◽  
Bárbara Leyton ◽  
Mónica González ◽  
Elizabeth Navarro ◽  
...  
Keyword(s):  
Celiac Disease ◽  
Nutritional Status ◽  
Gastrointestinal Symptoms ◽  
Failure To Thrive ◽  
Age At Diagnosis ◽  
Pediatric Hospitals ◽  
Geographical Variations ◽  
Number Of Patients ◽  
Antiendomysial Antibodies

The frequency of celiac disease (CD) has increased along time, with relevant changes reported in geographical variations, clinical presentation and nutritional repercussions. In recent years, some celiac patients are presenting overweight/obesity, but it is unclear how frequent this is and to what extent undernutrition remains a concern. This is relevant because CD tends to be overlooked in overweight patients. With this in mind, we assessed age at diagnosis, clinical characteristics and nutritional status of 155 celiac patients diagnosed between 1994–2017 in four pediatric hospitals in Santiago, Chile. Since 2003, the number of patients diagnosed has increased (p < 0.0033), coinciding with antitransglutaminase and antiendomysial antibodies becoming available to public health systems. In 2000, 4.5% of patients were asymptomatic at diagnosis, suggesting that active search is not routinely applied. Gastrointestinal symptoms plus failure to thrive were significantly more frequent under 2 years (p = 0.0001). Nutritional status has improved at diagnosis and during follow up, but undernutrition remains more frequent in children <2 and <5 years (p < 0.002 and p < 0.0036, respectively). Overweight at diagnosis was reported in 2002 and obesity in 2010. After initiating treatment, since 2010, patients changing from undernourishment to overweight has sometimes been observed after only 6 months on a gluten-free diet.

When Should One Ligate the Umbilical Cord?

PEDIATRICS ◽  
1972 ◽  
Vol 49 (6) ◽  
pp. 930-930
Author(s):  
John R. Haire
Keyword(s):  
Developing Countries ◽  
Infant Mortality ◽  
The Netherlands ◽  
Umbilical Cord ◽  
Normal Part ◽  
Vascular Bed ◽  
Low Incidence

Having observed and photographed obstetrical techniques and practices on six continents in 1971, I am struck by the differing views of renowned physicians in the various developed and developing countries as to the relative merits of early and late clamping of the umbilical cord. While specialists in the Netherlands, a country with a low incidence of infant mortality essentially equivalent to that of Sweden, stress the importance of placental transfusion as a normal part of the physiological sequence of birth which lessens the likelihood of maternal hemorrhage and anemia in the growing child, Yao et al. in their recent paper published in the December 1971 issue of PEDIATRICS, suggest that placental transfusion causes "overloading" of the vascular bed and therefore should be avoided by early clamping.

scholarly journals IgG Antibodies against Deamidated Gliadin Peptides for Diagnosis of Celiac Disease in Patients with IgA Deficiency

2010 ◽  
Vol 56 (3) ◽  
pp. 464-468 ◽  
Author(s):  
Danilo Villalta ◽  
Elio Tonutti ◽  
Christian Prause ◽  
Sibylle Koletzko ◽  
H Holm Uhlig ◽  
...  
Keyword(s):  
Celiac Disease ◽  
Blood Donors ◽  
Tissue Transglutaminase ◽  
Iga Deficiency ◽  
Diagnostic Sensitivity ◽  
Igg Antibodies ◽  
Diagnostic Specificity ◽  
Gliadin Peptides ◽  
Endomysium Antibodies ◽  
Monkey Esophagus

AbstractBackground: Assays for IgG antibodies against deamidated gliadin (IgG-anti-dGli) are comparable in performance with tests detecting IgA antibodies against tissue transglutaminase (IgA-anti-tTG) in diagnosing celiac disease (CD). IgA-anti-tTG are absent in IgA deficiency, a condition often associated with CD. In IgA deficiency, IgG-anti-tTG, which have a lower overall diagnostic accuracy, are routinely measured. We examined whether IgG-anti-dGli would be useful for diagnosing CD in patients with IgA deficiency.Methods: We studied 34 IgA-deficient CD patients, 185 IgA-competent newly diagnosed children with CD, 316 children without CD, 400 adult blood donors, and 6 control IgA-deficient individuals without CD. Anti-dGli and anti-tTG were measured by ELISA, and endomysium antibodies (EmA) were measured by immunofluorescence on monkey esophagus (IgA as well as IgG class for all antibodies). We calculated diagnostic sensitivity (percentage of patients above cutoff with 95% CIs) according to age-specific cutoffs for 95% diagnostic specificity and according to cutoffs proposed by the manufacturer of the assays.Results: No IgA-deficient CD patients were positive for any IgA-based antibody assay. Diagnostic sensitivity of IgG-anti-tTG was 91.2% (95% CI 76.3%–97.7%) according to age-specific cutoffs and 82.4% (66.1%–92.0%) according to manufacturer cutoffs. The diagnostic sensitivity of IgG-EmA was 75.8% (58.8%–87.4%) and the sensitivity of IgG-anti-dGli was 88.2% (72.8%–95.9%) according to both cutoffs.Conclusions: IgG-anti-dGli and IgG-anti-tTG have comparable diagnostic sensitivities for IgA-deficient celiac patients. IgG-anti-dGli may be useful for diagnosing CD in IgA-deficient patients.

Refeeding Syndrome in Children in Developing Countries Who Have Celiac Disease

2012 ◽  
Vol 54 (4) ◽  
pp. 521-524 ◽  
Author(s):  
Jaya Agarwal ◽  
Ujjal Poddar ◽  
Surender K. Yachha ◽  
Anshu Srivastava
Keyword(s):  
Developing Countries ◽  
Celiac Disease ◽  
Refeeding Syndrome

scholarly journals The effect of umbilical cord cleansing with chlorhexidine on omphalitis and neonatal mortality in community settings in developing countries: a meta-analysis

2013 ◽  
Vol 13 (Suppl 3) ◽  
pp. S15 ◽  
Author(s):  
Aamer Imdad ◽  
Luke C Mullany ◽  
Abdullah H Baqui ◽  
Shams El Arifeen ◽  
James M Tielsch ◽  
...  
Keyword(s):  
Developing Countries ◽  
Neonatal Mortality ◽  
Umbilical Cord ◽  
Meta Analysis ◽  
Community Settings
Sign in / Sign up

Export Citation Format

Share Document