Serum transferrin (total iron-binding capacity) in evaluation of iron status

Abstract We established a method for fully automated measurement of total iron-binding capacity (TIBC) in serum without separation of the unbound excess iron after saturating serum transferrin. After saturation of serum transferrin with an excess amount of iron (first step), the unbound iron was eliminated by formation of a complex with ferrozine, which was used as a chromogenic reagent (second step). For the TIBC assay, iron dissociated from transferrin by shifting the pH to acidic was reacted with ferrozine, and the increase in the absorbance at 570 nm was measured (third step). Because the iron used as a calibrator, which was added to saturate transferrin, reacted completely with ferrozine in the second step (elimination of unbound iron), the change in the absorbance to generate a calibration factor could not be monitored in the third step. To solve this problem, we used N-(2-hydroxyethyl)ethylenediamine-N,N′,N′-triacetic acid (HEDTA) to complex with the iron added to saturate transferrin in the second step. This made it possible to form an iron–ferrozine complex at acidic pH because iron was dissociated from HEDTA at acidic pH. The within-run CVs of this method were 0.66–2.43% at 17.7–77.0 μmol/L, and the day-to-day CVs were 1.06–1.57% at 29.9–60.4 μmol/L (n = 10). The correlation between the values obtained with this method (y) and those from the direct TIBC assay, which involved removal of unbound iron by ion-exchange resin (x), was: y = 0.963x + 0.29 μmol/L (r = 0.973, Sy|x = 2.83, n = 59), and with the TIBC values calculated from the serum iron concentrations and the unbound iron-binding capacities measured by a direct colorimetric method (x) was: y = 1.01x − 1.06 μmol/L (r = 0.994, Sy|x = 1.66, n = 51).

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Immunoturbidimetric determination of serum transferrin on a Kone Progress autoanalyser

Journal of Automatic Chemistry ◽

10.1155/s146392468900009x ◽

1989 ◽

Vol 11 (1) ◽

pp. 40-41 ◽

Cited By ~ 2

Author(s):

Luc Cynober ◽

Jacques Le Boucher ◽

Jacqueline Giboudeau

Keyword(s):

Binding Capacity ◽

Clinical Chemistry ◽

Total Iron ◽

Radial Immunodiffusion ◽

Serum Transferrin ◽

Iron Binding ◽

Total Iron Binding Capacity ◽

French Society ◽

Iron Binding Capacity

The Kone Progress, a multiparametric discrete analyser, was used to determine serum transferrin with a kit supplied by Kone. Assays recommended by the French Society of Clinical Chemistry were performed in order to assess the suitability of the test. Repeatability was assessed using serum pools with low (L), medium (M) and high (H) concentrations of transferrin. The coeffcients of variation (CV) were 5.4, 3.2 and 2.0% respectively for 30 determinations (within-batch). Reproducibility on 15 consecutive days (between-batch) was also satisfactory (CV for L = 7.3%, M = 6.3% and H = 3.8%). There was no serum-to-serum contamination. Results correlated closely with those obtained using radial immunodiffusion (RID) (r = 0.942) and total iron-binding capacity (r = 0.954)for 90 determinations.Transferrin measurement by immunoturbidimetry on the Kone Progress emerges as a well-suited, rapid and inexpensive alternative to other time-consuming (RID) and sophisticated (laser immunonephelemeter) techniques.

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Total Iron-binding Capacity Calculated from Serum Transferrin Concentration or Serum Iron Concentration and Unsaturated Iron-binding Capacity

Clinical Chemistry ◽

10.1373/49.1.175 ◽

2003 ◽

Vol 49 (1) ◽

pp. 175-178 ◽

Cited By ~ 42

Author(s):

Hachiro Yamanishi ◽

Shigeru Iyama ◽

Yoshihisa Yamaguchi ◽

Yuzuru Kanakura ◽

Yoshinori Iwatani

Keyword(s):

Serum Iron ◽

Binding Capacity ◽

Iron Concentration ◽

Total Iron ◽

Serum Transferrin ◽

Iron Binding ◽

Total Iron Binding Capacity ◽

Serum Iron Concentration ◽

Iron Binding Capacity

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Inflammatory diets are associated with lower total iron binding capacity in sera of young adults

International Journal for Vitamin and Nutrition Research ◽

10.1024/0300-9831/a000697 ◽

2021 ◽

pp. 1-9

Author(s):

Jeanne L. Doherty ◽

Doreen Y. Larvie ◽

Nitin Shivappa ◽

James R. Hebert ◽

Seth M. Armah

Keyword(s):

Young Adults ◽

Binding Capacity ◽

Iron Status ◽

Normal Weight ◽

Total Iron ◽

Iron Binding ◽

Total Iron Binding Capacity ◽

Total N ◽

Inflammatory Index ◽

Iron Binding Capacity

Abstract. Chronic, systemic inflammation, which is associated with obesity and numerous other diseases, impairs iron status by increasing hepcidin concentration. Inflammation also decreases the concentration of transferrin, the main iron transport protein and a negative acute phase protein, which is indirectly assessed by measuring total iron binding capacity (TIBC). However, the contribution of diet-induced inflammation has not been studied. Data from two studies, namely Diet and Inflammation and Selenium and Inflammation Studies (total n=98) were used to assess the associations among Dietary Inflammatory Index (DII®) scores derived from three-day dietary records, body mass index (BMI=weight[kg]/height[m]2), inflammatory and hematological markers among young adults with normal-weight, overweight or obesity. Subjects’ diets were also categorized as less inflammatory diets (LID) and inflammatory diets (ID) using cluster analysis. Independent t-test and regression analyses were used to assess associations in the data. Intakes of iron, proteins, fat, fiber, and calories were higher in the LID group compared to the ID group (p<0.05). Demographic characteristics and concentrations of C-reactive protein (CRP) and iron status biomarkers did not differ significantly between the two groups (p>0.05). Higher DII score was associated with increasing CRP (β+SE=0.23+0.07, p=0.002) and lower TIBC (β+SE=−8.46+3.44, p=0.02), independent of BMI category. The LID diet was associated with higher TIBC (β+SE=29.87+10.75, p=0.007) compared to the ID diet. In conclusion, inflammatory diets may impair iron status by reducing the iron binding capacity of transferrin.

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Evaluation of iron status in lemurs by analysis of serum iron and ferritin concentrations, total iron-binding capacity, and transferrin saturation

Journal of the American Veterinary Medical Association ◽

10.2460/javma.232.4.578 ◽

2008 ◽

Vol 232 (4) ◽

pp. 578-585 ◽

Cited By ~ 16

Author(s):

Cathy V. Williams ◽

Randall E. Junge ◽

Ilse H. Stalis

Keyword(s):

Serum Iron ◽

Binding Capacity ◽

Iron Status ◽

Transferrin Saturation ◽

Total Iron ◽

Iron Binding ◽

Total Iron Binding Capacity ◽

Iron Binding Capacity

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DEFISIENSI BESI PADA WANITA USIA SUBUR PRANIKAH OBESITAS

Media Gizi Mikro Indonesia ◽

10.22435/mgmi.v10i2.599 ◽

2019 ◽

Vol 10 (2) ◽

pp. 101-110

Author(s):

Fillah Fithra Dieny ◽

Nurmasari Widyastuti ◽

Deny Yudi Fitranti ◽

Choirun Nissa ◽

Fahmy Arif Tsani ◽

...

Keyword(s):

Random Sampling ◽

Binding Capacity ◽

Transferrin Saturation ◽

Total Iron ◽

Serum Transferrin ◽

Iron Binding ◽

Total Iron Binding Capacity ◽

Cross Sectional ◽

Systematic Random Sampling ◽

Iron Binding Capacity

Latar Belakang. Wanita usia subur (WUS) terutama di Indonesia saat ini menghadapi masalah gizi ganda yang kompleks. Obesitas merupakan salah satu masalah gizi yang paling banyak dialami oleh WUS. Anemia merupakan satu sisi lain dari masalah gizi ganda yang banyak dialami di negara berkembang. Obesitas diketahui memiliki hubungan dengan anemia pada WUS. Tujuan. Menganalisis status besi pada WUS pranikah dengan status gizi obesitas dan non obesitas. Metode. Penelitian ini merupakan penelitian observasional dengan desain cross-sectional yang dilakukan pada 50 mahasiswi Universitas Diponegoro, Semarang. Subjek berusia 18-25 tahun dan dipilih dengan metode systematic random sampling. Subjek terdiri dari 25 mahasiswi obesitas dan 25 mahasiswi non obesitas. Status besi diukur melalui serum besi, serum Transferrin Saturation (TSAT), dan Total Iron Binding Capacity (TIBC). Status obesitas dilihat melalui pengukuran antropometri persen lemak tubuh. Uji T tidak berpasangan digunakan untuk mengetahui perbedaan status besi pada WUS pranikah obesitas dan non obesitas. Hasil. Ada perbedaan kadar serum besi (p=0,027), TIBC (p=0,034), dan TSAT (p=0,004) antara kelompok obesitas dan non obesitas. Kadar serum besi kelompok obesitas lebih rendah, ditunjukkan dengan rerata sebesar 83,99±20,66 μg/dl pada kelompok obesitas, sedangkan kelompok non obesitas sebesar 99,2±26,03 μg/dl. Kadar TIBC kelompok obesitas lebih tinggi dilihat dari reratanya sebesar 420,98±47,22 μg/dl, sedangkan kelompok non obesitas 389,94±53,35 μg/dl. Pengukuran TSAT menunjukkan kelompok obesitas memiliki kadar yang lebih rendah ditunjukkan dengan reratanya 20,13±5,27%, sedangkan kelompok non obesitas memiliki rerata sebesar 25,99±7,92%. Kesimpulan. Kelompok obesitas memiliki rata-rata kadar serum besi dan TSAT lebih rendah serta rata-rata kadar TIBC lebih tinggi.

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