Total iron-binding capacity and serum transferrin determination under the influence of several clinical conditions

Abstract We established a method for fully automated measurement of total iron-binding capacity (TIBC) in serum without separation of the unbound excess iron after saturating serum transferrin. After saturation of serum transferrin with an excess amount of iron (first step), the unbound iron was eliminated by formation of a complex with ferrozine, which was used as a chromogenic reagent (second step). For the TIBC assay, iron dissociated from transferrin by shifting the pH to acidic was reacted with ferrozine, and the increase in the absorbance at 570 nm was measured (third step). Because the iron used as a calibrator, which was added to saturate transferrin, reacted completely with ferrozine in the second step (elimination of unbound iron), the change in the absorbance to generate a calibration factor could not be monitored in the third step. To solve this problem, we used N-(2-hydroxyethyl)ethylenediamine-N,N′,N′-triacetic acid (HEDTA) to complex with the iron added to saturate transferrin in the second step. This made it possible to form an iron–ferrozine complex at acidic pH because iron was dissociated from HEDTA at acidic pH. The within-run CVs of this method were 0.66–2.43% at 17.7–77.0 μmol/L, and the day-to-day CVs were 1.06–1.57% at 29.9–60.4 μmol/L (n = 10). The correlation between the values obtained with this method (y) and those from the direct TIBC assay, which involved removal of unbound iron by ion-exchange resin (x), was: y = 0.963x + 0.29 μmol/L (r = 0.973, Sy|x = 2.83, n = 59), and with the TIBC values calculated from the serum iron concentrations and the unbound iron-binding capacities measured by a direct colorimetric method (x) was: y = 1.01x − 1.06 μmol/L (r = 0.994, Sy|x = 1.66, n = 51).

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Immunoturbidimetric determination of serum transferrin on a Kone Progress autoanalyser

Journal of Automatic Chemistry ◽

10.1155/s146392468900009x ◽

1989 ◽

Vol 11 (1) ◽

pp. 40-41 ◽

Cited By ~ 2

Author(s):

Luc Cynober ◽

Jacques Le Boucher ◽

Jacqueline Giboudeau

Keyword(s):

Binding Capacity ◽

Clinical Chemistry ◽

Total Iron ◽

Radial Immunodiffusion ◽

Serum Transferrin ◽

Iron Binding ◽

Total Iron Binding Capacity ◽

French Society ◽

Iron Binding Capacity

The Kone Progress, a multiparametric discrete analyser, was used to determine serum transferrin with a kit supplied by Kone. Assays recommended by the French Society of Clinical Chemistry were performed in order to assess the suitability of the test. Repeatability was assessed using serum pools with low (L), medium (M) and high (H) concentrations of transferrin. The coeffcients of variation (CV) were 5.4, 3.2 and 2.0% respectively for 30 determinations (within-batch). Reproducibility on 15 consecutive days (between-batch) was also satisfactory (CV for L = 7.3%, M = 6.3% and H = 3.8%). There was no serum-to-serum contamination. Results correlated closely with those obtained using radial immunodiffusion (RID) (r = 0.942) and total iron-binding capacity (r = 0.954)for 90 determinations.Transferrin measurement by immunoturbidimetry on the Kone Progress emerges as a well-suited, rapid and inexpensive alternative to other time-consuming (RID) and sophisticated (laser immunonephelemeter) techniques.

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Total Iron-binding Capacity Calculated from Serum Transferrin Concentration or Serum Iron Concentration and Unsaturated Iron-binding Capacity

Clinical Chemistry ◽

10.1373/49.1.175 ◽

2003 ◽

Vol 49 (1) ◽

pp. 175-178 ◽

Cited By ~ 42

Author(s):

Hachiro Yamanishi ◽

Shigeru Iyama ◽

Yoshihisa Yamaguchi ◽

Yuzuru Kanakura ◽

Yoshinori Iwatani

Keyword(s):

Serum Iron ◽

Binding Capacity ◽

Iron Concentration ◽

Total Iron ◽

Serum Transferrin ◽

Iron Binding ◽

Total Iron Binding Capacity ◽

Serum Iron Concentration ◽

Iron Binding Capacity

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DEFISIENSI BESI PADA WANITA USIA SUBUR PRANIKAH OBESITAS

Media Gizi Mikro Indonesia ◽

10.22435/mgmi.v10i2.599 ◽

2019 ◽

Vol 10 (2) ◽

pp. 101-110

Author(s):

Fillah Fithra Dieny ◽

Nurmasari Widyastuti ◽

Deny Yudi Fitranti ◽

Choirun Nissa ◽

Fahmy Arif Tsani ◽

...

Keyword(s):

Random Sampling ◽

Binding Capacity ◽

Transferrin Saturation ◽

Total Iron ◽

Serum Transferrin ◽

Iron Binding ◽

Total Iron Binding Capacity ◽

Cross Sectional ◽

Systematic Random Sampling ◽

Iron Binding Capacity

Latar Belakang. Wanita usia subur (WUS) terutama di Indonesia saat ini menghadapi masalah gizi ganda yang kompleks. Obesitas merupakan salah satu masalah gizi yang paling banyak dialami oleh WUS. Anemia merupakan satu sisi lain dari masalah gizi ganda yang banyak dialami di negara berkembang. Obesitas diketahui memiliki hubungan dengan anemia pada WUS. Tujuan. Menganalisis status besi pada WUS pranikah dengan status gizi obesitas dan non obesitas. Metode. Penelitian ini merupakan penelitian observasional dengan desain cross-sectional yang dilakukan pada 50 mahasiswi Universitas Diponegoro, Semarang. Subjek berusia 18-25 tahun dan dipilih dengan metode systematic random sampling. Subjek terdiri dari 25 mahasiswi obesitas dan 25 mahasiswi non obesitas. Status besi diukur melalui serum besi, serum Transferrin Saturation (TSAT), dan Total Iron Binding Capacity (TIBC). Status obesitas dilihat melalui pengukuran antropometri persen lemak tubuh. Uji T tidak berpasangan digunakan untuk mengetahui perbedaan status besi pada WUS pranikah obesitas dan non obesitas. Hasil. Ada perbedaan kadar serum besi (p=0,027), TIBC (p=0,034), dan TSAT (p=0,004) antara kelompok obesitas dan non obesitas. Kadar serum besi kelompok obesitas lebih rendah, ditunjukkan dengan rerata sebesar 83,99±20,66 μg/dl pada kelompok obesitas, sedangkan kelompok non obesitas sebesar 99,2±26,03 μg/dl. Kadar TIBC kelompok obesitas lebih tinggi dilihat dari reratanya sebesar 420,98±47,22 μg/dl, sedangkan kelompok non obesitas 389,94±53,35 μg/dl. Pengukuran TSAT menunjukkan kelompok obesitas memiliki kadar yang lebih rendah ditunjukkan dengan reratanya 20,13±5,27%, sedangkan kelompok non obesitas memiliki rerata sebesar 25,99±7,92%. Kesimpulan. Kelompok obesitas memiliki rata-rata kadar serum besi dan TSAT lebih rendah serta rata-rata kadar TIBC lebih tinggi.

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