scholarly journals Method for determination of bone alkaline phosphatase activity: analytical performance and clinical usefulness in patients with metabolic and malignant bone diseases

1996 ◽  
Vol 42 (2) ◽  
pp. 210-217 ◽  
Author(s):  
W Withold ◽  
U Schulte ◽  
H Reinauer
Keyword(s):  
Alkaline Phosphatase ◽  
Bone Alkaline Phosphatase ◽  
Cross Reactivity ◽  
Bone Diseases ◽  
Characteristic Analysis ◽  
Diagnostic Efficacy ◽  
Alp Activity ◽  
Osteoblast Activity ◽  
Score Analysis

Abstract We report the performance characteristics of an assay for determination of bone alkaline phosphatase (ALP) activity after immunoadsorption in microplate wells. Between-run imprecision was between 7.1% and 11.2%. The detection limit was 1.0 U/L. Comparisons with an immunoradiometric test for determination of bone ALP mass concentrations yielded the following regression equation: y = 3.11 + 1.33x with y, the bone ALP activity concentration (U/L) (and x, the bone ALP mass concentration microgram/L) (r +=0.974, n = 103). Using sera from patients with liver diseases and sera from patients with secondary hyperparathyroidism yielded a cross-reactivity of 20% for circulating liver ALP (and its membrane-bound isoform). In patients receiving renal transplants, Z-score analysis revealed that after transplantation the increase in bone ALP activity is more pronounced than total ALP activity. In tumor patients, receiver-operating characteristic analysis revealed that bone ALP activity shows the same diagnostic efficacy as total ALP activity in the detection of bone metastases (as assessed by bone scintigraphy). In multiple myeloma patients, suppressed osteoblast activity was well detectable by bone ALP activity determination.

Immunoradiometric method and electrophoretic system compared for quantifying bone alkaline phosphatase in serum

1995 ◽  
Vol 41 (6) ◽  
pp. 853-857 ◽  
Author(s):  
V O Van Hoof ◽  
M Martin ◽  
P Blockx ◽  
A Prove ◽  
A Van Oosterom ◽  
...  
Keyword(s):  
Alkaline Phosphatase ◽  
Malignant Disease ◽  
Screening Method ◽  
Bone Alkaline Phosphatase ◽  
Cross Reactivity ◽  
Osteoblastic Activity ◽  
Alp Activity ◽  
Agarose Electrophoresis ◽  
End Stage ◽  
Rule Out

Abstract Agarose electrophoresis (Isopal, Beckman) and an immunoradiometric assay (IRMA) involving specific monoclonal antibodies (Ostase, Hybritech), two methods for the quantification of serum bone alkaline phosphatase (ALP, EC 3.1.3.1), a marker of osteoblastic activity, were compared in 293 patients: 79 with end-stage renal failure treated with hemodialysis and 214 with malignant disease. Overall correlation between the two methods was good (r = 0.92), except (a) for low values of bone ALP and (b) in some samples with high total liver ALP activity--both due to considerable cross-reactivity of the anti-bone ALP antibodies of the Ostase kit with liver ALP. This interference was not constant and was not evenly distributed across all concentrations of bone ALP. Low bone ALP determined with the IRMA (< or = 5 micrograms/L) was confirmed by electrophoresis (< or = 21 U/L), but bone ALP activity determined by electrophoresis to be low (< or = 21 U/L) was not correlated with the IRMA results. After standardizing our results by computing z-values for bone ALP, delta z (= zOstase - zIsopal) was significantly correlated with liver ALP activity (r = 0.73, P < 0.0001). We conclude that the IRMA for quantifying bone ALP is acceptable as a screening method. However, when high values for bone ALP are found with the Ostase method, confirmation by electrophoresis remains mandatory to rule out cross-reactivity with high amounts of liver ALP. For detecting low bone ALP activities, electrophoresis remains the method of choice.

A MnO2nanosheets–o-phenylenediamine oxidative system for the sensitive fluorescence determination of alkaline phosphatase activity

2018 ◽  
Vol 10 (44) ◽  
pp. 5341-5346 ◽  
Author(s):  
Xionghong Tan ◽  
Zheng Li ◽  
Yanlin Du ◽  
Aixian Zheng ◽  
Yongyi Zeng ◽  
...  
Keyword(s):  
Alkaline Phosphatase ◽  
Phosphatase Activity ◽  
Alkaline Phosphatase Activity ◽  
Alp Activity ◽  
Fluorescence Determination ◽  
Oxidative System

A MnO2nanosheets–o-phenylenediamine (OPDA) oxidative system was developed for detecting ALP activity selectively, sensitively and conveniently.

Bone alkaline phosphatase measured with a new immunoradiometric assay in patients with metabolic bone diseases

1996 ◽  
Vol 26 (5) ◽  
pp. 391-396 ◽  
Author(s):  
S. Gonnelli ◽  
C. Cepollaro ◽  
A. Montagnani ◽  
G. Monaci ◽  
M. S. Campagna ◽  
...  
Keyword(s):  
Alkaline Phosphatase ◽  
Bone Alkaline Phosphatase ◽  
Bone Diseases ◽  
Immunoradiometric Assay ◽  
Metabolic Bone Diseases ◽  
Metabolic Bone

scholarly journals Immunoadsorption assay for bone alkaline phosphatase compared with wheat germ agglutinin precipitation assay in serum from (pre)pubertal girls

1996 ◽  
Vol 42 (12) ◽  
pp. 1970-1974 ◽  
Author(s):  
A A Bouman ◽  
C M de Ridder ◽  
J H Nijhof ◽  
J C Netelenbos ◽  
H A Delemarre-vd Waal
Keyword(s):  
Alkaline Phosphatase ◽  
Correlation Coefficient ◽  
Wheat Germ Agglutinin ◽  
Wheat Germ ◽  
Bone Alkaline Phosphatase ◽  
Cross Reactivity ◽  
Reference Ranges ◽  
Correlation Studies ◽  
Deming Regression ◽  
The Mean

Abstract The performance characteristics of two bone alkaline phosphatase (ALP; EC 3.1.3.1) assays, a wheat germ agglutinin (WGA) precipitation assay and a new immunoadsorption assay (IAA), were compared. The within- and between-run imprecision of the IAA (3.6-4.2% and 3.6-7.7%) was comparable with that of the WGA assay. The mean cross-reactivity with liver ALP appeared to be 4% in the WGA assay and 11% in the IAA. The reference ranges in a group of 155 healthy Caucasian (pre)pubertal schoolgirls were: 149-401 U/L (total ALP, 30 degrees C), 105-349 U/L (bone ALP, 30 degrees C, WGA assay), and 58-205 U/L (bone ALP, 25 degrees C, IAA). Comparison of the WGA assay (x) with the IAA (y) demonstrated a correlation coefficient of 0.95 [Deming regression equation: y = (0.56 +/- 0.01)x + (2.0 +/- 1.5); Sy[symbol: see text]x = 5.3 U/L]. Correlation studies of the WGA assay and the IAA results with total ALP demonstrated r = 0.98 and 0.96, respectively.

Quantification of bone alkaline phosphatase in serum by precipitation with wheat-germ lectin: a simplified method and its clinical plausibility.

1986 ◽  
Vol 32 (10) ◽  
pp. 1960-1966 ◽  
Author(s):  
W Behr ◽  
J Barnert
Keyword(s):  
Alkaline Phosphatase ◽  
Wheat Germ ◽  
Biliary Tree ◽  
Bone Alkaline Phosphatase ◽  
Original Method ◽  
Bone Diseases ◽  
Skeletal Involvement ◽  
Number Of Patients ◽  
Comparison Groups ◽  
Wheat Germ Lectin

Abstract We report an easy, rapid method for quantifying bone isoenzyme of alkaline phosphatase (EC 3.1.3.1., ALP) in serum. The original method described by Rosalki and Ying Foo (Clin Chem 1984;30:1182-6) was somewhat simplified. In contrast to their results, we found that bone ALP is precipitated quantitatively by wheat-germ lectin. To check the clinical plausibility of the method, we used samples from several comparison groups (blood donors, children, pregnant women, patients with neoplasms but without skeletal involvement) and a large number of patients suffering from bone diseases and diseases of the liver and biliary tree. Measured activities of bone ALP nearly always correlated with the clinical diagnosis. Only patients with hepatitis often had pathological bone activities not in accord with the other findings. Possible reasons for this observation are discussed.

scholarly journals Lung sonography is useful for the initial detection of left-sided double lumen tracheal tube position in both novice and expert; a randomized prospective crossover study

2019 ◽  
Author(s):  
Hyungseok Seo ◽  
YoonJu Go ◽  
Yong Seok Jang ◽  
Bong-Jae Lee
Keyword(s):  
Double Lumen Tube ◽  
Characteristic Analysis ◽  
Double Lumen ◽  
Diagnostic Efficacy ◽  
Predictive Values ◽  
Lung Sonography ◽  
Chest Auscultation ◽  
Novices And Experts ◽  
Sensitivity Specificity

Abstract Background Detecting the position of the double lumen tube (DLT), lung sonography, can be an effective technique and may provide more detailed information than chest auscultation. However, the diagnostic efficacy of lung sonography for determining DLT position can differ between novice and expert.Methods We enrolled and allocated ninety patients into two groups; one group using chest auscultation and the other using lung sonography for the determination of DLT position. In both groups, two repeat-assessments were provided by two independent examiners; first by a novice, and second by an expert. The primary outcome was the overall accuracy, sensitivity, specificity, positive or negative predictive values between novice and expert in confirming the position of the left-sided DLT. In both groups, final position was confirmed by a fiberoptic bronchoscopy.Results Both using auscultation and sonography, novices and experts showed similar diagnostic efficacy. However, in patients using sonography, both novice and expert showed better outcomes than in patients using chest auscultation. In receiver operating characteristic analysis, lung sonography seemed to showe a better predictability in incorrect DLT position than chest auscultation, especially by experts.Conclusion Lung sonography showed a better diagnostic efficacy for detecting DLT position than chest auscultation. Furthermore, using lung sonography, novice investigator may perform similar assessment for identifying incorrect DLT position to expert.

Bone alkaline phosphatase on the IDS-iSYS automated analyser; cross-reactivity with intestinal ALP

2019 ◽  
Vol 57 (8) ◽  
pp. e186-e188 ◽  
Author(s):  
Jennifer J. Brady ◽  
Desmond McGoldrick ◽  
Kate O’Callaghan ◽  
Fiona McNamara ◽  
Keith J. Mulready ◽  
...  
Keyword(s):  
Alkaline Phosphatase ◽  
Bone Alkaline Phosphatase ◽  
Cross Reactivity
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