scholarly journals Clinical Utility of a Wheat-Germ Precipitation Assay for Determination of Bone Alkaline Phosphatase Concentrations in Patients with Different Metabolic Bone Diseases

1995 ◽  
Vol 33 (7) ◽  
Author(s):  
Vania Braga ◽  
Romolo Dorizzi ◽  
Giorgio Brocco ◽  
Maurizio Rossini ◽  
Nicoletta Zamberlan ◽  
...  
Keyword(s):  
Alkaline Phosphatase ◽  
Clinical Utility ◽  
Wheat Germ ◽  
Bone Alkaline Phosphatase ◽  
Bone Diseases ◽  
Metabolic Bone Diseases ◽  
Metabolic Bone

Bone alkaline phosphatase measured with a new immunoradiometric assay in patients with metabolic bone diseases

1996 ◽  
Vol 26 (5) ◽  
pp. 391-396 ◽  
Author(s):  
S. Gonnelli ◽  
C. Cepollaro ◽  
A. Montagnani ◽  
G. Monaci ◽  
M. S. Campagna ◽  
...  
Keyword(s):  
Alkaline Phosphatase ◽  
Bone Alkaline Phosphatase ◽  
Bone Diseases ◽  
Immunoradiometric Assay ◽  
Metabolic Bone Diseases ◽  
Metabolic Bone

Serum levels of bone alkaline phosphatase measured with a new immunoradiometric assay in patients with metabolic bone diseases

Bone ◽  
1995 ◽  
Vol 16 (3) ◽  
pp. 409
Author(s):  
S. Gonnelli ◽  
C. Cepollaro ◽  
M.S. Campagna ◽  
M.B. Franci ◽  
A. Monaci ◽  
...  
Keyword(s):  
Alkaline Phosphatase ◽  
Serum Levels ◽  
Bone Alkaline Phosphatase ◽  
Bone Diseases ◽  
Immunoradiometric Assay ◽  
Metabolic Bone Diseases ◽  
Metabolic Bone

Triple-Photon Energy Absorptiometry in the Measurement of Bone Mineral

1988 ◽  
Vol 29 (4) ◽  
pp. 461-464 ◽  
Author(s):  
R. Jonson ◽  
B. Roos ◽  
T. Hansson
Keyword(s):  
Soft Tissue ◽  
Bone Mineral ◽  
Theoretical Basis ◽  
Mineral Content ◽  
Bone Diseases ◽  
Attenuation Coefficients ◽  
Metabolic Bone Diseases ◽  
Metabolic Bone ◽  
Mass Attenuation Coefficients

The feasibility of determination of bone mineral content using a triple-photon absorptiometric technique is presented. Using three different photon energies it is possible to correct the measured ‘bone mineral’ value for fat and lean soft tissue. The theoretical basis of the method, as well as the experimental determination of mass attenuation coefficients, is described. It is shown that the results are reproducible within 5 per cent, which means that the method could be of value in the management of metabolic bone diseases.

Quantification of bone alkaline phosphatase in serum by precipitation with wheat-germ lectin: a simplified method and its clinical plausibility.

1986 ◽  
Vol 32 (10) ◽  
pp. 1960-1966 ◽  
Author(s):  
W Behr ◽  
J Barnert
Keyword(s):  
Alkaline Phosphatase ◽  
Wheat Germ ◽  
Biliary Tree ◽  
Bone Alkaline Phosphatase ◽  
Original Method ◽  
Bone Diseases ◽  
Skeletal Involvement ◽  
Number Of Patients ◽  
Comparison Groups ◽  
Wheat Germ Lectin

Abstract We report an easy, rapid method for quantifying bone isoenzyme of alkaline phosphatase (EC 3.1.3.1., ALP) in serum. The original method described by Rosalki and Ying Foo (Clin Chem 1984;30:1182-6) was somewhat simplified. In contrast to their results, we found that bone ALP is precipitated quantitatively by wheat-germ lectin. To check the clinical plausibility of the method, we used samples from several comparison groups (blood donors, children, pregnant women, patients with neoplasms but without skeletal involvement) and a large number of patients suffering from bone diseases and diseases of the liver and biliary tree. Measured activities of bone ALP nearly always correlated with the clinical diagnosis. Only patients with hepatitis often had pathological bone activities not in accord with the other findings. Possible reasons for this observation are discussed.

scholarly journals Radiographic imaging of metabolic bone disorders and their relative management

2016 ◽  
Vol 5 (1) ◽  
pp. 264
Author(s):  
Parveen Chandna ◽  
Pramod Setty J. ◽  
Jeevika M. U. ◽  
Praveen Kumar T. Kochar ◽  
Siddesh M. B.
Keyword(s):  
Alkaline Phosphatase ◽  
Serum Calcium ◽  
Structural Integrity ◽  
Serum Alkaline Phosphatase ◽  
Bone Diseases ◽  
The Body ◽  
Cell Physiology ◽  
Radiographic Imaging ◽  
Metabolic Bone Diseases ◽  
Metabolic Bone

Background: Bone is a strong dynamic organ of the endoskeleton playing a vital role in structural integrity envisaging to keep proper shape and maintenance of the body, mineral reservoirs, blood production, coagulation and immunity. Metabolic bone diseases are a heterogeneous group of disorders that interrupt the normal homeostasis of bone formation and resorption. Bone regulates as well as acts as a host for hematopoiesis by providing niche for proliferation and differentiation of hematopoietic cell. Bone is a dynamic tissue but metabolically active as it is being constantly formed (modelling) and reformed (remodelling). Metabolic bone diseases comprise of a broad spectrum of inherited and acquired disorders characterized by abnormalities in calcium metabolism and bone cell physiology- that lead to an altered serum calcium concentration and skeletal failure.Methods: After taking a properly informed written consent and complete history, thorough clinical examination was done and these patients were subjected to radiographic imaging and biochemical analysis.Results: Serum alkaline phosphatase is a good marker in rickets and osteomalacia, ICTP in osteoporosis, pyridinoline, deoxypyridinoline in primary hyperparathyroidism, serum PICP in renal osteodystrophy.Conclusions: In cases of rickets and osteomalacia either decreased or normal values of serum calcium and serum phosphorus were obtained. But the cases pertaining to renal failure with rickets values of serum phosphorous were found to be raised. However, in all cases of rickets and osteomalacia values of serum alkaline phosphatase were also found to be raised.

scholarly journals Method for determination of bone alkaline phosphatase activity: analytical performance and clinical usefulness in patients with metabolic and malignant bone diseases

1996 ◽  
Vol 42 (2) ◽  
pp. 210-217 ◽  
Author(s):  
W Withold ◽  
U Schulte ◽  
H Reinauer
Keyword(s):  
Alkaline Phosphatase ◽  
Bone Alkaline Phosphatase ◽  
Cross Reactivity ◽  
Bone Diseases ◽  
Characteristic Analysis ◽  
Diagnostic Efficacy ◽  
Alp Activity ◽  
Osteoblast Activity ◽  
Score Analysis

Abstract We report the performance characteristics of an assay for determination of bone alkaline phosphatase (ALP) activity after immunoadsorption in microplate wells. Between-run imprecision was between 7.1% and 11.2%. The detection limit was 1.0 U/L. Comparisons with an immunoradiometric test for determination of bone ALP mass concentrations yielded the following regression equation: y = 3.11 + 1.33x with y, the bone ALP activity concentration (U/L) (and x, the bone ALP mass concentration microgram/L) (r +=0.974, n = 103). Using sera from patients with liver diseases and sera from patients with secondary hyperparathyroidism yielded a cross-reactivity of 20% for circulating liver ALP (and its membrane-bound isoform). In patients receiving renal transplants, Z-score analysis revealed that after transplantation the increase in bone ALP activity is more pronounced than total ALP activity. In tumor patients, receiver-operating characteristic analysis revealed that bone ALP activity shows the same diagnostic efficacy as total ALP activity in the detection of bone metastases (as assessed by bone scintigraphy). In multiple myeloma patients, suppressed osteoblast activity was well detectable by bone ALP activity determination.

scholarly journals Comparison of total and bone-specific alkaline phosphatase in patients with nonskeletal disorder or metabolic bone diseases

1996 ◽  
Vol 42 (11) ◽  
pp. 1796-1804 ◽  
Author(s):  
H W Woitge ◽  
M J Seibel ◽  
R Ziegler
Keyword(s):  
Alkaline Phosphatase ◽  
Colorimetric Assay ◽  
Clinical Information ◽  
Reference Interval ◽  
Bone Diseases ◽  
Cross Sectional ◽  
Metabolic Bone Diseases ◽  
Specific Alkaline Phosphatase ◽  
Bone Specific Alkaline Phosphatase ◽  
Metabolic Bone

Abstract To evaluate the diagnostic validity of new assays for bone-specific alkaline phosphatase (BAP), we compared measurements of total alkaline phosphatase (TAP) in serum with results for three different assays of serum BAP in healthy adults (n = 119), patients with chronic nonskeletal disorders (n = 123), and patients with metabolic bone diseases (n = 113). Serum TAP was determined by a standard colorimetric assay, BAP by the methods of lectin precipitation (L-BAP), enzyme immunoassay (E-BAP), and immunoradiometric assay (I-BAP). Impairment of liver function resulted in significant increases of all alkaline phosphatase (AP) measurements, with the smallest changes being exhibited by E-BAP. Compared with the results by TAP, diagnostic sensitivity (i.e., of values exceeding the reference interval) was not improved by BAP, but receiver-operating characteristic (ROC) curve analyses revealed improved discrimination for primary hyperparathyroidism by E-BAP. These results indicate that, in the presence of liver disease, the specificity of AP measurements is improved by measuring BAP. In most other clinical situations, serum TAP appears to provide sufficient clinical information; however, the cross-sectional study design used here allows no statement about the usefulness of BAP in serial measurements.

Is oral health correlated with skeletal phenotype in primary metabolic bone diseases? A preliminary report of the Greek experience

2019 ◽  
Author(s):  
Artemis Doulgeraki ◽  
Margarita Gatzogianni ◽  
Andreas Agouropoulos ◽  
Helen Athanasopoulou ◽  
Georgios Polyzois ◽  
...  
Keyword(s):  
Oral Health ◽  
Preliminary Report ◽  
Bone Diseases ◽  
Metabolic Bone Diseases ◽  
Metabolic Bone ◽  
Skeletal Phenotype
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