Analytic Methods for Bilirubin in Blood Plasma

Abstract A critical evaluation of existing colorimetric and spectrophotometric methods for the determination of bilirubin in blood plasma is presented. The stoichiometric equivalences of the reagents used in the diazo coupling reaction are re-evaluated. The elimination of turbidity is discussed, as well as methods using diazo-coupling promoters. Recommendations are made concerning the preservation of specimens to prevent oxidation and procedures that give maximal specificity for the type of bilirubin pigment being assayed.

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Green methods for the determination of nitrite in water samples based on a novel diazo coupling reaction

Green Processing and Synthesis ◽

10.1515/gps-2016-0005 ◽

2017 ◽

Vol 6 (2) ◽

Author(s):

Safwan M. Fraihat

Keyword(s):

Water Samples ◽

Acidic Medium ◽

Coupling Reaction ◽

Spectrophotometric Methods ◽

New Methods ◽

Diazo Coupling ◽

Colored Product ◽

Diazo Coupling Reaction

AbstractGreen, sensitive and selective spectrophotometric methods were developed for the determination of nitrite in water samples. The methods (A, B and C) are based on the reduction of 5-nitroimidazoles; metronidazole (MTL), secnidazole (SCL) and tinidazole (TNL), respectively, followed by diazotization using nitrite in acidic medium, then coupling with pyrogallol (PG). The amount of nitrite was determined by measuring the absorbance of the colored product at 385 nm. The new methods were linear in the ranges of 2.5–30 μgml

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A multicommuted flow analysis method for the photometric determination of amoxicillin in pharmaceutical formulations using a diazo coupling reaction

Journal of the Brazilian Chemical Society ◽

10.1590/s0103-50532011000200013 ◽

2011 ◽

Vol 22 (2) ◽

pp. 279-285 ◽

Cited By ~ 3

Author(s):

Sueny K. B Freitas ◽

Valdinete Lins da Silva ◽

Alberto N Araújo ◽

Maria Conceição B. S. M Montenegro ◽

Boaventura F Reis ◽

...

Keyword(s):

Flow Analysis ◽

Coupling Reaction ◽

Pharmaceutical Formulations ◽

Photometric Determination ◽

Analysis Method ◽

Diazo Coupling ◽

Diazo Coupling Reaction

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Diazo coupling reaction for the determination of biliverdin.

BUNSEKI KAGAKU ◽

10.2116/bunsekikagaku.40.8_377 ◽

1991 ◽

Vol 40 (8) ◽

pp. 377-381

Author(s):

Yuji SUZUKI ◽

Yoshikatsu SAKAGISHI

Keyword(s):

Coupling Reaction ◽

Diazo Coupling ◽

Diazo Coupling Reaction

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Spectrophotometric Determination of Oxymetazoline Hydrochloride in Pure and Pharmaceutical Preparations Using Diazo-coupling Reaction

JOURNAL OF EDUCATION AND SCIENCE ◽

10.33899/edusj.1999.163319 ◽

2019 ◽

Vol 28 (4) ◽

pp. 224-232

Author(s):

Thabit Al-Ghabsha ◽

Aseel Obedagha ◽

Rawaa Zakaria

Keyword(s):

Spectrophotometric Determination ◽

Pharmaceutical Preparations ◽

Coupling Reaction ◽

Diazo Coupling ◽

Diazo Coupling Reaction

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Spectrophotometric determination of total proteins in blood plasma: a comparative study among dye-binding methods

Brazilian Archives of Biology and Technology ◽

10.1590/s1516-89132005000300008 ◽

2005 ◽

Vol 48 (3) ◽

pp. 385-388 ◽

Cited By ~ 14

Author(s):

Dimas Augusto Morozin Zaia ◽

Fábio Rangel Marques ◽

Cássia Thaïs Bussamra Vieira Zaia

Keyword(s):

Comparative Study ◽

Blood Plasma ◽

Total Protein ◽

Total Proteins ◽

Erythrosin B ◽

Spectrophotometric Methods ◽

Biuret Method ◽

Bradford Method ◽

In Blood Plasma

A comparative study between the biuret method (standard method for total proteins) and spectrophotometric methods using dyes (Bradford, 3',3",5',5"-tetrabromophenolphthalein ethyl ester-TBPEE, and erythrosin-B) was carried out for the determination of total proteins in blood plasma from rats. Bradford method showed the highest sensitivity for proteins and biuret method showed the lowest. For all the methods, the absorbance for different proteins (BSA, casein, and egg albumin) was measured and Bradford method showed the lowest variation of absorbance. The concentration of total protein obtained by using Bradford method was not statistically different (p>0.05) from concentration of total protein obtained by the biuret method. But in regard to erythrosin-B and TBPEE methods the concentrations of total protein were statistically different (p<0.05). Thus, Bradford method could be used instead of the biuret method for determination of total proteins in blood plasma.

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