scholarly journals False-Positive Results of an Enzyme Immunoassay for Rubella IgM in a Case of Measles

1997 ◽  
Vol 24 (2) ◽  
pp. 271-272 ◽  
Author(s):  
S. M. Donovan
Keyword(s):  
Enzyme Immunoassay ◽  
False Positive ◽  
Positive Results

Low Rate of False-Positive Results with Use of A Rapid HIV Test

2002 ◽  
Vol 23 (6) ◽  
pp. 335-337 ◽  
Author(s):  
Cassandra D. Salgado ◽  
Heidi L. Flanagan ◽  
Doris M. Haverstick ◽  
Barry M. Farr
Keyword(s):  
Enzyme Immunoassay ◽  
False Positive ◽  
Diagnostic System ◽  
Rapid Test ◽  
Occupational Exposures ◽  
High Rate ◽  
Negative Results ◽  
Hiv Test ◽  
Rapid Hiv Test ◽  
Positive Results

Background:Occupational exposure to human immunodeficiency virus (HIV) is an important threat to healthcare workers. Centers for Disease Control and Prevention guidelines recommend prompt institution of prophylaxis. This requires (1) immediate prophylaxis after exposure, pending test results that may take more than 24 hours in many hospitals; or (2) performance of a rapid test. The Single Use Diagnostic System (SUDS)® HIV-1 Test is used to screen rapidly for antibodies to HIV type 1 in plasma or serum, with a reported sensitivity of more than 99.9%. We used this test from January 1999 until September 2000, when it was withdrawn from the market following reports claiming a high rate of false-positive results.Methods:We reviewed the results of postexposure HIV testing during 21 months.Results:A total of 884 SUDS tests were performed on source patients after occupational exposures (883 negative results, 1 reactive result). The results of repeat SUDS testing on the reactive specimen were also reactive, but the results of enzyme immunoassay and Western blot testing were negative. A new specimen from the same patient showed a negative result on SUDS testing. This suggested a specificity of 99.9%. In the 4 months after SUDS testing was suspended, there was 1 false-positive result on enzyme immunoassay for 1 of 132 source patients (presumed specificity, 99.2%).Conclusion:Use of the SUDS test facilitated rapid and accurate evaluation of source specimens, obviating unnecessary prophylaxis.

scholarly journals False-Positive Results Obtained with the Alexon ProSpecT Cryptosporidium Enzyme Immunoassay

1999 ◽  
Vol 37 (5) ◽  
pp. 1582-1583 ◽  
Author(s):  
Kirk M. Doing ◽  
Jill L. Hamm ◽  
Jo Ann Jellison ◽  
Jessica A. Marquis ◽  
Cindy Kingsbury
Keyword(s):  
Enzyme Immunoassay ◽  
False Positive ◽  
Antigen Detection ◽  
Immunocompromised Patients ◽  
Careful Attention ◽  
Iodine Staining ◽  
Negative Results ◽  
Food Borne ◽  
Positive Results

Cryptosporidium is known to cause diarrhea in immunocompromised patients and is also associated with outbreaks of disease due to food-borne and waterborne parasites. Traditional procedures, involving iodine staining of wet mounts of stool sediments and trichrome staining, lack the sensitivity to detectCryptosporidium. Special staining procedures, such as the modified acid-fast and safranin stains, are generally employed. Less labor-intensive antigen detection assays have simplified detection; however, careful attention to local epidemiology is important because false-positive tests occur. Here, we report two incidents involving 62 false-positive results obtained with the Alexon ProSpecTCryptosporidium enzyme immunoassay, which were deemed false-positive based on negative results obtained from extensive microscopic examinations.

scholarly journals Histoplasmosis-Associated Cross-Reactivity in the BioRad Platelia Aspergillus Enzyme Immunoassay

2007 ◽  
Vol 14 (5) ◽  
pp. 638-640 ◽  
Author(s):  
L. Joseph Wheat ◽  
Emily Hackett ◽  
Michelle Durkin ◽  
Patricia Connolly ◽  
Ruta Petraitiene ◽  
...  
Keyword(s):  
Enzyme Immunoassay ◽  
Experimental Infection ◽  
False Positive ◽  
Second Generation ◽  
Cross Reactivity ◽  
Positive Results

ABSTRACT We observed false-positive results in the Platelia Aspergillus enzyme-linked immunoassay (EIA) for specimens from patients with histoplasmosis and mice with experimental infection. Platelia Aspergillus EIA-positive specimens were negative in the second-generation Histoplasma antigen EIA. Care must be taken to exclude histoplasmosis for patients with positive Platelia Aspergillus EIA results.

scholarly journals Experience of application of ELISA test kids for the serological diagnosis of West Nile fever

2014 ◽  
Vol 19 (2) ◽  
pp. 59-62
Author(s):  
A. R. Azaryan ◽  
A. P. Grishanova ◽  
E. I. Ivashchenko ◽  
G. L. Shendo ◽  
A. I. Kovtunov ◽  
...  
Keyword(s):  
West Nile Virus ◽  
Enzyme Immunoassay ◽  
False Positive ◽  
Elisa Test ◽  
Serological Diagnosis ◽  
West Nile Fever ◽  
West Nile ◽  
Biotech Company ◽  
Positive Results

As a result of comparative application of enzyme immunoassay "Anti-West Nile virus ELISA-IgM" of the company "Euroimmun AG" (Germany) and "BioScrin-WNV-IgM", produced by ZAO Biotech company "Bioservice» (Russia), intended for serodiagnosis of West Nile fever there was established very low specificity of "Anti-West Nile ELISA-IgM", which is manifested in the registration of false positive results.

A Collaborative Study on the Detection of Staphylococcal Enterotoxins in Foods with an Enzyme Immunoassay Kit (TECRA)

1996 ◽  
Vol 59 (4) ◽  
pp. 390-397 ◽  
Author(s):  
C. E. PARK ◽  
D. WARBURTON ◽  
P. J. LAFFEY ◽  
Keyword(s):  
Enzyme Immunoassay ◽  
False Positive ◽  
Collaborative Study ◽  
False Negative ◽  
Negative Control ◽  
Rabbit Serum ◽  
Staphylococcal Enterotoxins ◽  
Repeatability And Reproducibility ◽  
Positive Results ◽  
Control Samples

One of the commercially available enzyme immunoassay kits for the detection of staphylococcal enterotoxins (SEs) in foods, the TECRA screening kit (Bioenterprises Pty. Ltd., Roseville, New South Wales, Australia), has microtiter plates coated with a mixture of antibodies to all of the SEs. A collaborative study was conducted to ascertain whether specificity, sensitivity, repeatability, and reproducibility of the results obtained using this kit would meet food-safety criteria. Thirteen Canadian collaborators participated in this study to analyze both various foods to which 1.0 to 3.0 ng of SE/g of food had been added and negative control samples. In addition, the effect of animal serum in these analyses was examined. The results indicate that all collaborators (100%) were able to detect the minimum toxin levels of 1.0 ng of SEA/g of ham and 1.0 ng of SEB/g of salami and SE or SEs in other samples (chicken, turkey, and cheese) containing 2.0 to 3.0 ng/g, without any false-negative results. With regard to negative control samples, all collaborators obtained correct results except when analyzing two types of food: two collaborators (15%) showed weak false-positive results with salami and all analysts found strong false-positive results with mussels. The problem regarding specificity could be largely corrected by treating the sample with rabbit serum (0.1 volume in 1.0 volume food extract). The repeatability and reproducibility of results from the kit were acceptable.

scholarly journals Common Herbal Supplements Did Not Produce False-Positive Results on Urine Drug Screens Analyzed by Enzyme Immunoassay

2004 ◽  
Vol 28 (4) ◽  
pp. 272-273 ◽  
Author(s):  
John S. Markowitz ◽  
Jennifer L. Donovan ◽  
C. Lindsay DeVane ◽  
Kenneth D. Chavin
Keyword(s):  
Enzyme Immunoassay ◽  
False Positive ◽  
Herbal Supplements ◽  
Urine Drug ◽  
Positive Results

scholarly journals Can Aspergillus fumigatus conidia cause false-positive results in the galactomannan enzyme immunoassay test?

2018 ◽  
Vol 51 (3) ◽  
pp. 387-389
Author(s):  
Ângela Leitzke Cabana ◽  
Josiara Furtado Mendes ◽  
Gabriel Baracy Klafke ◽  
Tchana Martinez Brandolt ◽  
Aryse Martins Melo ◽  
...  
Keyword(s):  
Aspergillus Fumigatus ◽  
Enzyme Immunoassay ◽  
False Positive ◽  
Positive Results

scholarly journals Response to a Report on False-Positive Results in a Methadone Enzyme Immunoassay

1998 ◽  
Vol 44 (10) ◽  
pp. 2221-2222 ◽  
Author(s):  
Yuh-geng Tsay ◽  
Parisa Khosropour
Keyword(s):  
Enzyme Immunoassay ◽  
False Positive ◽  
Positive Results

scholarly journals Evaluation of OIDx Histoplasma Urinary Antigen EIA

2021 ◽  
Author(s):  
Diego Hernando Caceres ◽  
Beatriz L. Gomez ◽  
Angela M. Tobon ◽  
Tom Chilller ◽  
Mark D. Lindsley
Keyword(s):  
Enzyme Immunoassay ◽  
False Positive ◽  
High Sensitivity ◽  
Reference Panel ◽  
Urine Samples ◽  
Urinary Antigen ◽  
Imaging Diagnostics ◽  
Sandwich Enzyme Immunoassay ◽  
Positive Results ◽  
Optimum Imaging

Abstract A sandwich enzyme immunoassay (EIA) for the detection of Histoplasma antigens (Ag) in urine, developed by Optimum Imaging Diagnostics (OIDx) was evaluated. A verification using a standardized reference panel of urine samples found sensitivity of 92%, specificity of 32% and accuracy of 51%. In this study, the OIDx Histoplasma urinary Ag EIA displayed high sensitivity, however, in non-histoplasmosis cases this EIA displayed false-positive results in 68% of specimens tested.

Sign in / Sign up

Export Citation Format

Share Document