AbstractWe used the microarray data of poplar gene in the laboratory and the GPDNN model to preprocess the data and conduct the median adjustment, and then studied and analyzed the phloem and xylem genes with good clustering effect. Among them, a gene that was significantly different and related to plant hormones was selected for analysis. Firstly, the function of this gene was predicted to be auxin response factor. All genes of auxin response factor family were selected from poplar transcription factor database for evolutionary and homologous affinity analysis, and the relationship with homology was analyzed according to the expression semaphore correlation between genes. Finally, the number of up-regulated, down-regulated and fine-tuned genes in the tissues of budding, root, xylem and phloem was selected by using the median, and the relationship between the number of up-regulated genes and their semaphores and auxin content in the tissues was analyzed.