Effects of low magnitude high frequency mechanical vibration combined with compressive force on human periodontal ligament cells in vitro

In the present investigation, four titanium (Ti) surfaces of dental implants were compared through in vitro systems. The surface roughness of Ti was measured by TR240 mobile surface roughmeter. The Ti implants were seeded with human periodontal ligament cells (hPLDCs) and maintained for a period of 0-7 days. The adhesion, proliferation, and differentiation of hPLDCs were observed by using Cell morphology, cell counting and Osteocalcin (OC) immunofluorescent staining. Results suggest that surface roughness of titanium favors hPDLCs behavior and improves cell adhesion, proliferation, and differentiation.

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Human periodontal ligament cells express osteoblastic phenotypes under intermittent force loading in vitro

Frontiers in Bioscience ◽

10.2741/1835 ◽

2006 ◽

Vol 11 (1) ◽

pp. 776 ◽

Cited By ~ 33

Author(s):

Y., Q. Yang

Keyword(s):

Periodontal Ligament ◽

Periodontal Ligament Cells ◽

Force Loading ◽

Human Periodontal Ligament Cells

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Cyclic tensile force stimulates BMP9 synthesis and in vitro mineralization by human periodontal ligament cells

Journal of Cellular Physiology ◽

10.1002/jcp.27257 ◽

2018 ◽

Vol 234 (4) ◽

pp. 4528-4539 ◽

Cited By ~ 7

Author(s):

Yanee Tantilertanant ◽

Jitti Niyompanich ◽

Vincent Everts ◽

Pitt Supaphol ◽

Prasit Pavasant ◽

...

Keyword(s):

Periodontal Ligament ◽

Tensile Force ◽

Periodontal Ligament Cells ◽

Human Periodontal Ligament Cells ◽

In Vitro Mineralization

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Force-induced decline of FOXM1 in human periodontal ligament cells contributes to osteoclast differentiation

The Angle Orthodontist ◽

10.2319/072418-536.1 ◽

2019 ◽

Vol 89 (5) ◽

pp. 804-811 ◽

Cited By ~ 1

Author(s):

Qian Li ◽

Jianyun Zhang ◽

Dawei Liu ◽

Yunan Liu ◽

Yanheng Zhou

Keyword(s):

Transcription Factors ◽

Periodontal Ligament ◽

Osteoclast Differentiation ◽

Specific Inhibitor ◽

Compressive Force ◽

Nuclear Factor Κb ◽

Periodontal Ligament Cells ◽

Tartrate Resistant Acid Phosphatase ◽

Proliferation Capacity ◽

Human Periodontal Ligament Cells

ABSTRACT Objectives: To investigate whether Forkhead family transcription factors are responsive to mechanical force and the resulting influence on the osteoclast differentiation mediated by human periodontal ligament cells (PDLCs). Materials and Methods: A high-throughput RNA sequencing assay was performed in compressive force–stimulated and control human PDLCs. Alteration of FOXM1, a member of the Forkhead family transcription factors, was further confirmed by Western blotting and quantitative reverse-transcription polymerase chain reaction. Expression of FOXM1 was inhibited by either small interfering RNA (siRNA) transfection or addition of its specific inhibitor Siomycin A. Then, cells were exposed to compressive force and co-cultured with the murine macrophage cell line Raw264.7, followed by tartrate-resistant acid phosphatase staining assay. Expression changes of receptor activator of nuclear factor κB ligand (RANKL) and osteoprotegetin (OPG) caused by FOXM1 suppression were measured. Alkaline phosphatase (ALP) staining, ALP activity assay, and crystal violet staining assay were performed after FOXM1 inhibition. Results: FOXM1 transcription decreased after mechanical stimulation in PDLCs. Inhibition of FOXM1 promoted force-induced osteoclast differentiation of RAW264.7 and upregulated the RANKL/OPG ratio in PDLCs. Interference of FOXM1 led to promoted osteogenic differentiation but decreased proliferation of PDLCs. Conclusions: FOXM1 is a novel mechano-responsive gene in human PDLCs. Suppressing FOXM1 expression could promote osteoclast differentiation as well as RANKL/OPG in human PDLCs. FOXM1 also plays a role in controlling PDLC differentiation and proliferation capacity.

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