The effects of red ginseng saponin fraction-A (RGSF-A) on phagocytosis and intracellular signaling in Brucella abortus infected RAW 264.7 cells

Asthma allergic disease is caused by airway chronic inflammation. Some intracellular signaling pathways, such as MAPK and STAT3-SOCS3, are involved in the control of airway inflammation in asthma. The flavonoid sakuranetin demonstrated an anti-inflammatory effect in different asthma models. Our aim was to clarify how sakuranetin treatment affects MAPK and STAT3-SOCS3 pathways in a murine experimental asthma model. Mice were submitted to an asthma ovalbumin-induction protocol and were treated with vehicle, sakuranetin, or dexamethasone. We assayed the inflammatory profile, mucus production, and serum antibody, STAT3-SOCS3, and MAPK levels in the lungs. Morphological alterations were also evaluated in the liver. LPS-stimulated RAW 264.7 cells were used to evaluate the effects of sakuranetin on nitric oxide (NO) and cytokine production. In vivo, sakuranetin treatment reduced serum IgE levels, lung inflammation (eosinophils, neutrophils, and Th2/Th17 cytokines), and respiratory epithelial mucus production in ovalbumin-sensitized animals. Considering possible mechanisms, sakuranetin inhibits the activation of ERK1/2, JNK, p38, and STAT3 in the lungs. No alterations were found in the liver for treated animals. Sakuranetin did not modify in vitro cell viability in RAW 264.7 and reduced NO release and gene expression of IL-1β and IL-6 induced by LPS in these cells. In conclusion, our data showed that the inhibitory effects of sakuranetin on eosinophilic lung inflammation can be due to the inhibition of Th2 and Th17 cytokines and the inhibition of MAPK and STAT3 pathways, reinforcing the idea that sakuranetin can be considered a relevant candidate for the treatment of inflammatory allergic airway disease.

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Red Ginseng Saponin Fraction A Isolated from Korean Red Ginseng by Ultrafiltration on the Porcine Coronary Artery

Journal of Ginseng Research ◽

10.5142/jgr.2011.35.3.325 ◽

2011 ◽

Vol 35 (3) ◽

pp. 325-330 ◽

Cited By ~ 8

Author(s):

Young-Hyun Jung ◽

Kwang-Yeol Park ◽

Jin-Hong Jeon ◽

Yi-Seong Kwak ◽

Yong-Bum Song ◽

...

Keyword(s):

Coronary Artery ◽

Red Ginseng ◽

Korean Red Ginseng ◽

Porcine Coronary Artery ◽

Ginseng Saponin ◽

Saponin Fraction

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Emodin Successfully Inhibited Invasion of Brucella abortus Via Modulting Adherence, Microtubule Dynamics and ERK Signaling Pathway in RAW 264.7 Cells

Journal of Microbiology and Biotechnology ◽

10.4014/jmb.1804.04040 ◽

2018 ◽

Vol 28 (10) ◽

pp. 1723-1729 ◽

Cited By ~ 1

Author(s):

Tran Xuan Ngoc Huy ◽

Alisha Wehdnesday Bernardo Reyes ◽

Huynh Tan Hop ◽

Lauren Togonon Arayan ◽

Vu Hai Son ◽

...

Keyword(s):

Signaling Pathway ◽

Brucella Abortus ◽

Microtubule Dynamics ◽

Erk Signaling ◽

Raw 264.7 Cells ◽

Raw 264.7

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Global gene-expression profiles of intracellular survival of the BruAb2_1031 gene mutated Brucella abortus in professional phagocytes, RAW 264.7 cells

BMC Microbiology ◽

10.1186/s12866-018-1223-7 ◽

2018 ◽

Vol 18 (1) ◽

Cited By ~ 3

Author(s):

Myunghwan Jung ◽

Soojin Shim ◽

Young Bin Im ◽

Woo Bin Park ◽

Han Sang Yoo

Keyword(s):

Gene Expression ◽

Brucella Abortus ◽

Expression Profiles ◽

Gene Expression Profiles ◽

Global Gene Expression ◽

Intracellular Survival ◽

Raw 264.7 Cells ◽

Raw 264.7

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Korean Red Ginseng Saponin Fraction Rich in Ginsenoside-Rb1, Rc and Rb2 Attenuates the Severity of Mouse Collagen-Induced Arthritis

Mediators of Inflammation ◽

10.1155/2014/748964 ◽

2014 ◽

Vol 2014 ◽

pp. 1-14 ◽

Cited By ~ 17

Author(s):

Mehari Endale ◽

Eun Ju Im ◽

Joo Young Lee ◽

Sung Dae Kim ◽

Taddesse Yayeh ◽

...

Keyword(s):

Inflammatory Responses ◽

Combined Therapy ◽

Cartilage Damage ◽

Serum Antibody ◽

Ginsenoside Rb1 ◽

Red Ginseng ◽

Korean Red Ginseng ◽

Ginseng Saponin ◽

Anti Inflammatory ◽

Saponin Fraction

Despite a multitude of reports on anti-inflammatory properties of ginseng extracts or individual ginsenosides, data on antiarthritic effect of ginseng saponin preparation with mixed ginsenosides is limited. On the other hand, a combined therapy of safe and inexpensive plant-derived natural products such as ginsenosides can be considered as an alternative to treat arthritis. Our previousin vitrodata displayed a strong anti-inflammatory action of red ginseng saponin fraction-A (RGSF-A). We, herein, report a marked antiarthritic property of RGSF-A rich in ginsenoside Rb1, Rc, and Rb2. Collagen-induced arthritic (CIA) mice were treated with RGSF-A or methotrexate (MTX) for 5 weeks. Joint pathology, serum antibody production and leukocye activation, cytokine production in the circulation, lymph nodes, and joints were examined. RGSF-A markedly reduced severity of arthritis, cellular infiltration, and cartilage damage. It suppressed CD3+/CD69+, CD4+/CD25+, CD8+T-cell, CD19+, B220/CD23+B-cell, MHCII+/CD11c+, and Gr-1+/CD11b+cell activations. It further suppressed anti-CII- or anti-RF-IgG/IgM, TNF-α, IL-1β, IL-17, and IL-6 secretions but stimulated IL-10 levels in the serum, joint, or splenocyte. RGSF-A attenuated arthritis severity, modified leukocyte activations, and restored cytokine imbalances, suggesting that it can be considered as an antiarthritic agent with the capacity to ameliorate the immune and inflammatory responses in CIA mice.

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