Controlled Moisture Stress to Improve Cold Hardiness and Morphology of Douglas-fir Seedlings

1979 ◽  
Vol 25 (4) ◽  
pp. 576-582
Author(s):  
John Blake ◽  
Joe Zaerr ◽  
Stephen Hee

Abstract Nursery-grown seedlings of Douglas-fir (Pseudotsuga menziesii) were subjected to three levels of moisture stress between late July and the end of August. Measurements in October and December showed that mild stress of -5 to -10 bars significantly improved cold hardiness. In a second experiment, the effectiveness of the mild stress treatment decreased as the onset of the stress was delayed from mid-July until September 1. Furthermore, the earlier onset of stress decreased the height and shoot/root ratio, but increased the root weight and nitrogen content of the needles. For seedlings lifted in October, mild stress also decreased mortality from cold storage. Forest Sci. 25:576-582.


2015 ◽  
Vol 21 (10) ◽  
pp. 3814-3826 ◽  
Author(s):  
Sheel Bansal ◽  
J. Bradley St. Clair ◽  
Constance A. Harrington ◽  
Peter J. Gould


1990 ◽  
Vol 5 (3) ◽  
pp. 86-89 ◽  
Author(s):  
Ole T. Helgerson

Abstract Douglas-fir (Pseudotsuga menziesii) container-grown plug (1-0) and nursery-grown bareroot (2-0) seedlings were planted in southwest Oregon beneath sclerophyll hardwoods injected with triclopyr amine or left untreated. Sixty percent of the hardwood canopy was killed by injection. Seedlings beneath treated hardwoods experienced greater daytime moisture stress but lower predawn moisture stress. Plugs grew more in the first year than bareroots. Five-year survival averaged 100% and 96% for plugs and bareroots under treated hardwoods, 83% and 73% under untreated hardwoods. Herbicide injection of hardwoods was associated with increased height, diameter, and volume growth rates in the underplanted Douglas-fir and seems necessary for establishing Douglas-fir in this environment. West. J. Appl. For. 5(3):86-89, July 1990.



1993 ◽  
Vol 71 (10) ◽  
pp. 1280-1290 ◽  
Author(s):  
Joanne E. MacDonald ◽  
John N. Owens

Bud development in response to different dormancy-induction treatments in coastal Douglas-fir (Pseudotsuga menziesii (Mirb.) Franco var. menziesii) seedlings was investigated under commercial greenhouse conditions. There were three treatments: short day without moisture stress, short day with moisture stress, and long day with moisture stress. Within the first week in the short day treatments, neoformed-leaf initiation ended and bud-scale initiation began and ended. Rapid leaf initiation began in week 1 and was completed by week 5 for the short day with moisture stress treatment and week 6 for the short day without moisture stress treatment. Slow leaf initiation was completed by week 13. Crown cells became apparent within the pith during weeks 3–6; cell walls thickened between weeks 8 and 13. Within the first week of the long day with moisture stress treatment, neoformed-leaf initiation ended and bud-scale initiation began. Bud-scale initiation ended by week 3 or 4. Then slow leaf initiation began and continued until week 6. Rate of leaf initiation was rapid during weeks 8–10 and decreased slightly during weeks 10–13. By week 13, apical height had decreased markedly, indicating an imminent end to leaf initiation. Crown cells became apparent within the pith during weeks 5–13; cell walls thickened between weeks 10 and 13. Key words: bud development, dormancy induction, short days, moisture stress, Douglas-fir, seedlings.



Genetics ◽  
2009 ◽  
Vol 183 (1) ◽  
pp. 289-298 ◽  
Author(s):  
Andrew J. Eckert ◽  
Jill L. Wegrzyn ◽  
Barnaly Pande ◽  
Kathleen D. Jermstad ◽  
Jennifer M. Lee ◽  
...  




1998 ◽  
Vol 18 (10) ◽  
pp. 671-679 ◽  
Author(s):  
S. Guak ◽  
D. M. Olsyzk ◽  
L. H. Fuchigami ◽  
D. T. Tingey


2000 ◽  
Vol 30 (5) ◽  
pp. 837-840 ◽  
Author(s):  
T S Anekonda ◽  
W T Adams ◽  
S N Aitken ◽  
D B Neale ◽  
K D Jermstad ◽  
...  

Variation in cold-hardiness traits, and their extent of genetic control and interrelationships, were investigated among individuals (clones) within a single large full-sib family of coastal Douglas-fir (Pseudotsuga menziesii var. menziesii (Mirb.) Franco) from Oregon. Cold injury to needle, stem, and bud tissues was evaluated in fall 1996 and spring 1997 following artificial freeze testing of detached shoots collected from 4-year-old ramets (rooted cuttings). Variation among clones in cold-injury scores was significant (p < 0.01) for all shoot tissues in both fall and spring and averaged about three times the magnitude previously observed among open-pollinated families of this species. Thus, improving cold hardiness by within-family selection appears to hold much promise. Striking similarities in relative magnitudes of heritability estimates and genetic correlations in the full-sib family, compared with breeding populations, support the following hypotheses about the quantitative genetics of cold hardiness in this species: (i) heritability of cold hardiness (both broad-and-narrow-sense) is stronger in the spring than in the fall; (ii) cold hardiness of different shoot tissues in the same season is controlled by many of the same genes; and (iii) genetic control of fall cold hardiness is largely independent of cold hardiness in the spring.



1980 ◽  
Vol 10 (3) ◽  
pp. 371-378 ◽  
Author(s):  
W. C. Carlson ◽  
W. D. Binder ◽  
C. O. Feenan ◽  
C. L. Preisig

A method is presented for rapid determination of the mitotic index in terminal buds of Douglas-fir, Pseudotsugamenziesii (Mirb.) Franco. Fertilization at planting increased mitotic index but fertilized seedlings entered dormancy at the same time as unfertilized seedlings. Cold storage, prior to dormancy, reduced mitotic index rapidly and was related to reduced height growth the following year. The effects of moderate moisture stress and seed source on mitotic index were slight.



2001 ◽  
Vol 16 (2) ◽  
pp. 71-79 ◽  
Author(s):  
Todd M. Birchler ◽  
Robin Rose ◽  
Diane L. Haase

Abstract Coastal Douglas-fir (Pseudotsuga menziesii) 1+1 seedlings were fertilized with two fertilizers [NH4NO3+K2SO4 and (NH4)2SO4+KCl] at four rates (0, 80, 160, 320 kg N and K/ha) split over three application dates (September 19, October 13, November 1, 1996). Fertilizer type did not affect total Kjeldahl nitrogen (TKN) levels on any of the sampling dates. By January 10, TKN concentrations had increased 16, 30, and 34%, and chloride concentrations had increased 57, 77, and 112% relative to the unfertilized seedlings for the 80, 160, and 320 kg N+K/ha treatments, respectively. Nitrate levels increased briefly after the first application of NH4NO3+K2SO4. Potassium levels remained relatively unchanged. Levels of most other nutrients, as well as foliar dry weight, increased between September 16 and January 10, but these increases were generally unrelated to the fertilizer treatments. Root growth potential and cold hardiness did not differ among treatments. Seedlings that received 160 or 320 kg N/ha broke bud an average of 3 days earlier than did the unfertilized seedlings. Chlorophyll fluorescence (Fv/Fm) of fertilized seedlings was consistently higher than that of unfertilized seedlings on November 13 and December 30. These treatment differences were not reflected in seedling outplanting performance after one growing season. West. J. Appl. For. 16(2):71–79.



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