scholarly journals Evolutionary aspects of the S-related genes of the Brassica self-incompatibility system: synonymous and nonsynonymous base substitutions.

Genetics ◽  
1995 ◽  
Vol 140 (3) ◽  
pp. 1099-1104 ◽  
Author(s):  
K Hinata ◽  
M Watanabe ◽  
S Yamakawa ◽  
Y Satta ◽  
A Isogai
Keyword(s):  
Self Incompatibility ◽  
Receptor Kinase ◽  
Operational Taxonomic Units ◽  
Incompatibility System ◽  
S Alleles ◽  
Nonself Recognition ◽  
Evolutionary Aspects ◽  
S Locus Glycoprotein ◽  
Receptor Domain ◽  
Allelic Differentiation

Abstract In the Brassicaceae, self-vs. nonself-recognition in self-incompatibility is controlled by sporophytic S-alleles. Haplotypes specifying both SRK (S-receptor kinase) and SLG (S-locus glycoprotein) are considered to play an important role in the recognition reactions. We compared the nucleotide sequences of SRK9(Bc) and SRK6(Bo). The number of nonsynonymous substitutions per site (Pn) was lower, constrained, in the kinase than the receptor domain, while the numbers of synonymous substitutions (Ps) in the two domains were largely comparable. Pairwise values for Ps and Pn were calculated among 17 operational taxonomic units, including eight SLGs, the receptor domains of two SRKs, four SRAs (S-related A) and three SRBs (S-related B), which have high homologies with each other. The values of Ps and Pn of SLG were mostly comparable to those of the receptor domain of SRK. Dendrograms constructed on the basis of Pn and Ps indicated that SRA differentiated first, followed by SRB. The differentiation of SLG alleles is one of prerequisite factors for the establishment of self-incompatibility, and the allelic differentiation has occurred more than tens of million years ago.

scholarly journals Nonself-recognition-based self-incompatibility can alternatively promote or prevent introgression

2020 ◽  
Author(s):  
Alexander Harkness ◽  
Yaniv Brandvain
Keyword(s):  
Pollen Limitation ◽  
List Type ◽  
Self Incompatibility ◽  
Core Eudicots ◽  
S Alleles ◽  
Self Fertilization ◽  
Nonself Recognition ◽  
Negative Frequency Dependent Selection ◽  
Incompatibility Alleles ◽  
S Allele

1SummaryTraditionally, we expect that self-incompatibility alleles (S-alleles), which prevent self-fertilization, should benefit from negative-frequency dependent selection and rise to high frequency when introduced to a new population through gene flow. However, the most taxonomically widespread form of self-incompatibility, the ribonuclease-based system ancestral to the core eudicots, functions through nonself-recognition, which drastically alters the process of S-allele diversification.We analyze a model of S-allele evolution in two populations connected by migration, focusing on comparisons among the fates of S-alleles originally unique to each population and those shared among populations.We find that both shared and unique S-alleles originating from the population with more unique S-alleles were usually fitter than S-alleles from the population with fewer. Resident S-alleles were often driven extinct and replaced by migrant S-alleles, though this outcome could be averted by pollen limitation or biased migration.Nonself-recognition-based self-incompatibility will usually either disfavor introgression of S-alleles or result in the whole-sale replacement of S-alleles from one population with those from another.

scholarly journals When gametophytic self-incompatibility meets gynodioecy

2008 ◽  
Vol 90 (1) ◽  
pp. 27-35 ◽  
Author(s):  
BODIL K. EHLERS ◽  
MIKKEL H. SCHIERUP
Keyword(s):  
Inbreeding Depression ◽  
Self Incompatibility ◽  
S Locus ◽  
Selfing Rate ◽  
Female Function ◽  
Fecundity Selection ◽  
Incompatibility System ◽  
S Alleles ◽  
Self Compatibility

SummaryThe occurrence of gynodioecy among angiosperms appears to be associated with self-compatibility. We use individual-based simulations to investigate the conditions for breakdown of a gametophytic self-incompatibility system in gynodioecious populations and make a comparison with hermaphroditic populations where the conditions are well known. We study three types of mutations causing self-compatibility. We track the fate of these mutations in both gynodioecious and hermaphroditic populations, where we vary the number of S-alleles, inbreeding depression and selfing rate. We find that the conditions for breakdown are less stringent if the population is gynodioecious and that the breakdown of self-incompatibility tends to promote stability of gynodioecious populations since it results in a higher frequency of females. We also find that fecundity selection has a large effect on the probability of breakdown of self-incompatibility, in particular if caused by a mutation destroying the female function of the S-locus.

Pollen flow in distylous populations of Amsinckia (Boraginaceae)

1976 ◽  
Vol 54 (22) ◽  
pp. 2530-2535 ◽  
Author(s):  
Fred R. Ganders
Keyword(s):  
Pollen Flow ◽  
Progeny Testing ◽  
Self Incompatibility ◽  
Disassortative Mating ◽  
Pollen Loads ◽  
Self Pollination ◽  
Incompatibility System ◽  
Total Pollen

Stigmatic pollen loads were analyzed from naturally pollinated pin and thrum form flowers of Amsinckia douglasiana and A. vernicosa var. furcata. Pin stigmas captured more total pollen than thrum stigmas. Pins experienced either net self-pollination or random pollination. Thrum stigmas experienced significant disassortative pollination. Comparing pollen loads from intact and emasculated thrum flowers of A. douglasiana indicated that self-pollination and geitonogamy were relatively unimportant in the pollination of the thrum form. The level of disassortative pollination of A. vernicosa var. furcata does not appear to be high enough to account for the level of disassortative mating observed by progeny testing, suggesting that this species may possess an incomplete stylar self-incompatibility system such as has been reported in A. grandiflora.

Identification and characterization of BoPUB3: a novel interaction protein with S-locus receptor kinase in Brassica oleracea L.

2019 ◽  
Vol 51 (7) ◽  
pp. 723-733 ◽  
Author(s):  
Songmei Shi ◽  
Qiguo Gao ◽  
Tonghong Zuo ◽  
Zhenze Lei ◽  
Quanming Pu ◽  
...  
Keyword(s):  
Brassica Oleracea ◽  
Signaling Pathway ◽  
Chromosomal Region ◽  
The Self ◽  
Mature Stage ◽  
Self Incompatibility ◽  
Receptor Kinase ◽  
S Locus ◽  
Phylogenic Tree ◽  
Brassica Oleracea L

Abstract Armadillo repeat containing 1 (ARC1) is phosphorylated by S-locus receptor kinase (SRK) and functions as a positive regulator in self-incompatibility response of Brassica. However, ARC1 only causes partial breakdown of the self-incompatibility response, and other SRK downstream factors may also participate in the self-incompatibility signaling pathway. In the present study, to search for SRK downstream targets, a plant U-box protein 3 (BoPUB3) was identified from the stigma of Brassica oleracea L. BoPUB3 was highly expressed in the stigma, and its expression was increased with the stigma development and reached to the highest level in the mature-stage stigma. BoPUB3, a 76.8-kDa protein with 697 amino acids, is a member of the PUB-ARM family and contains three domain characteristics of BoARC1, including a U-box N-terminal domain, a U-box motif, and a C-terminal arm repeat domain. The phylogenic tree showed that BoPUB3 was close to BoARC1. The synteny analysis revealed that B. oleracea chromosomal region containing BoPUB3 had high synteny with the Arabidopsis thaliana chromosomal region containing AtPUB3 (At3G54790). In addition, the subcellular localization analysis showed that BoPUB3 primarily localized in the plasma membrane and also in the cytoplasm. The combination of the yeast two-hybrid and in vitro binding assay showed that both BoPUB3 and BoARC1 could interact with SRK kinase domain, and SRK showed much higher level of β-galactosidase activity in its interaction with BoPUB3 than with BoARC1. These results implied that BoPUB3 is a novel interactor with SRK, which lays a basis for further research on whether PUB3 participates in the self-incompatibility signaling pathway.

Sign in / Sign up

Export Citation Format

Share Document