scholarly journals A Transgene Encoding a Plasma Membrane H+-ATPase That Confers Acid Resistance in Arabidopsis thaliana Seedlings

Genetics ◽  
1998 ◽  
Vol 149 (2) ◽  
pp. 501-507
Author(s):  
Jeff C Young ◽  
Natalie D DeWitt ◽  
Michael R Sussman
Keyword(s):  
Arabidopsis Thaliana ◽  
Plasma Membrane ◽  
Large Body ◽  
Acid Resistance ◽  
Transport Systems ◽  
Proton Pumps ◽  
Ph Homeostasis ◽  
Higher Plant ◽  
Large Gene

Abstract Proton pumps (H+-ATPases) are the primary active transport systems in the plasma membrane of higher plant cells. These enzymes are encoded by a large gene family expressed throughout the plant, with specific isoforms directed to various specialized cells. While their involvement in membrane energetics has been suggested by a large body of biochemical and physiological studies, a genetic analysis of their role in plants has not yet been performed. We report here that mutant Arabidopsis thaliana plants containing a phloem-specific transgene encoding a plasma membrane H+-ATPase with an altered carboxy terminus show improved growth at low pH during seedling development. These observations provide the first genetic evidence for a role of the plasma membrane H+-ATPase in cytoplasmic pH homeostasis in plants.

The role of polyamines in the regulation of the plasma membrane and the tonoplast proton pumps under salt stress

2010 ◽  
Vol 167 (4) ◽  
pp. 261-269 ◽  
Author(s):  
MaŁgorzata Janicka-Russak ◽  
Katarzyna KabaŁa ◽  
Ewa MŁodzińska ◽  
Grażyna KŁobus
Keyword(s):  
Plasma Membrane ◽  
Salt Stress ◽  
Proton Pumps

scholarly journals The role of plasma membrane H + ‐ ATP ase in jasmonate‐induced ion fluxes and stomatal closure in Arabidopsis thaliana

2015 ◽  
Vol 83 (4) ◽  
pp. 638-649 ◽  
Author(s):  
Suli Yan ◽  
Eric S. McLamore ◽  
Shanshan Dong ◽  
Haibo Gao ◽  
Masashige Taguchi ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Plasma Membrane ◽  
Stomatal Closure ◽  
Ion Fluxes

scholarly journals Plant Proton Pumps and Cytosolic pH-Homeostasis

2021 ◽  
Vol 12 ◽  
Author(s):  
Maike Cosse ◽  
Thorsten Seidel
Keyword(s):  
Plasma Membrane ◽  
Secretory Pathway ◽  
Coordination Mechanisms ◽  
Proton Pumps ◽  
Plasma Membrane Atpase ◽  
Ph Homeostasis ◽  
Secondary Active Transport ◽  
Cytosolic Ph ◽  
Ion Concentrations ◽  
Membrane Atpase

Proton pumps create a proton motif force and thus, energize secondary active transport at the plasma nmembrane and endomembranes of the secretory pathway. In the plant cell, the dominant proton pumps are the plasma membrane ATPase, the vacuolar pyrophosphatase (V-PPase), and the vacuolar-type ATPase (V-ATPase). All these pumps act on the cytosolic pH by pumping protons into the lumen of compartments or into the apoplast. To maintain the typical pH and thus, the functionality of the cytosol, the activity of the pumps needs to be coordinated and adjusted to the actual needs. The cellular toolbox for a coordinated regulation comprises 14-3-3 proteins, phosphorylation events, ion concentrations, and redox-conditions. This review combines the knowledge on regulation of the different proton pumps and highlights possible coordination mechanisms.

Dual Role of Plasma Membrane Electron Transport Systems in Defense

2000 ◽  
Vol 35 (3) ◽  
pp. 197-220 ◽  
Author(s):  
Antonio del Castillo-Olivares ◽  
Ignacio Núñez de Castro ◽  
Miguel Ángel Medina
Keyword(s):  
Plasma Membrane ◽  
Electron Transport ◽  
Dual Role ◽  
Transport Systems ◽  
Plasma Membrane Electron Transport

Plasma Membrane Aquaporin Members PIPs Act in Concert to Regulate Cold Acclimation and Freezing Tolerance Responses in Arabidopsis thaliana

2020 ◽  
Vol 61 (4) ◽  
pp. 787-802 ◽  
Author(s):  
Arifa Rahman ◽  
Yukio Kawamura ◽  
Masayoshi Maeshima ◽  
Abidur Rahman ◽  
Matsuo Uemura
Keyword(s):  
Arabidopsis Thaliana ◽  
Plasma Membrane ◽  
Cold Acclimation ◽  
Freezing Tolerance ◽  
Cell Biology ◽  
Integrated Approach ◽  
Freezing Stress ◽  
Single Mutation ◽  
Plasma Membrane Intrinsic Proteins

Abstract Aquaporins play a major role in plant water uptake at both optimal and environmentally stressed conditions. However, the functional specificity of aquaporins under cold remains obscure. To get a better insight to the role of aquaporins in cold acclimation and freezing tolerance, we took an integrated approach of physiology, transcript profiling and cell biology in Arabidopsis thaliana. Cold acclimation resulted in specific upregulation of PIP1;4 and PIP2;5 aquaporin (plasma membrane intrinsic proteins) expression, and immunoblotting analysis confirmed the increase in amount of PIP2;5 protein and total amount of PIPs during cold acclimation, suggesting that PIP2;5 plays a major role in tackling the cold milieu. Although single mutants of pip1;4 and pip2;5 or their double mutant showed no phenotypic changes in freezing tolerance, they were more sensitive in root elongation and cell survival response under freezing stress conditions compared with the wild type. Consistently, a single mutation in either PIP1;4 or PIP2;5 altered the expression of a number of aquaporins both at the transcriptional and translational levels. Collectively, our results suggest that aquaporin members including PIP1;4 and PIP2;5 function in concert to regulate cold acclimation and freezing tolerance responses.

scholarly journals Brassinosteroids Regulate Plasma Membrane Anion Channels in Addition to Proton Pumps During Expansion of Arabidopsis thaliana Cells

2005 ◽  
Vol 46 (9) ◽  
pp. 1494-1504 ◽  
Author(s):  
Zongshen Zhang ◽  
Javier Ramirez ◽  
David Reboutier ◽  
Mathias Brault ◽  
Jacques Trouverie ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Plasma Membrane ◽  
Proton Pumps ◽  
Anion Channels

scholarly journals Diverse Physiological Functions of Cation Proton Antiporters across Bacteria and Plant Cells

2020 ◽  
Vol 21 (12) ◽  
pp. 4566
Author(s):  
Masaru Tsujii ◽  
Ellen Tanudjaja ◽  
Nobuyuki Uozumi
Keyword(s):  
Tertiary Structure ◽  
Plant Cells ◽  
Proton Motive Force ◽  
Motive Force ◽  
Transport Systems ◽  
Ph Homeostasis ◽  
Cell Organelles ◽  
Regulatory Domains ◽  
Selective Filter

Membrane intrinsic transport systems play an important role in maintaining ion and pH homeostasis and forming the proton motive force in the cytoplasm and cell organelles. In most organisms, cation/proton antiporters (CPAs) mediate the exchange of K+, Na+ and Ca2+ for H+ across the membrane in response to a variety of environmental stimuli. The tertiary structure of the ion selective filter and the regulatory domains of Escherichia coli CPAs have been determined and a molecular mechanism of cation exchange has been proposed. Due to symbiogenesis, CPAs localized in mitochondria and chloroplasts of eukaryotic cells resemble prokaryotic CPAs. CPAs primarily contribute to keeping cytoplasmic Na+ concentrations low and controlling pH, which promotes the detoxification of electrophiles and formation of proton motive force across the membrane. CPAs in cyanobacteria and chloroplasts are regulators of photosynthesis and are essential for adaptation to high light or osmotic stress. CPAs in organellar membranes and in the plasma membrane also participate in various intracellular signal transduction pathways. This review discusses recent advances in our understanding of the role of CPAs in cyanobacteria and plant cells.

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