Mapping Unexplored Genomes: A Genetic Linkage Map of the Hawaiian Cricket Laupala

Genetics ◽  
2002 ◽  
Vol 162 (3) ◽  
pp. 1275-1282 ◽  
Author(s):  
Y M Parsons ◽  
K L Shaw
Keyword(s):  
Linkage Map ◽  
Genomic Dna ◽  
Genetic Linkage ◽  
Genetic Linkage Map ◽  
Fragment Length Polymorphism ◽  
Linkage Groups ◽  
Marker System ◽  
Map Construction ◽  
Species Specific ◽  
Genomic Map

Abstract As with many organisms of evolutionary interest, the Hawaiian cricket Laupala genome is not well characterized genetically. Mapping such an unexplored genome therefore presents challenges not often faced in model genetic organisms and not well covered in the literature. We discuss the evolutionary merits of Laupala as a model for speciation studies involving prezygotic change, our choice of marker system for detecting genetic variation, and the initial genetic expectations pertaining to the construction of any unknown genomic map in general and to the Laupala linkage map construction in particular. We used the technique of amplified fragment length polymorphism (AFLP) to develop a linkage map of Laupala. We utilized both EcoRI/MseI- and EcoRI/PstI-digested genomic DNA to generate AFLP bands and identified 309 markers that segregated among F2 interspecific hybrid individuals. The map is composed of 231 markers distributed over 11 and 7 species-specific autosomal groups together with a number of putative X chromosome linkage groups. The integration of codominant markers enabled the identification of five homologous linkage groups corresponding to five of the seven autosomal chromosomal pairs found in Laupala.

scholarly journals Genetic Linkage Map of the Edible BasidiomycetePleurotus ostreatus

2000 ◽  
Vol 66 (12) ◽  
pp. 5290-5300 ◽  
Author(s):  
Luis M. Larraya ◽  
G�mer P�rez ◽  
Enrique Ritter ◽  
Antonio G. Pisabarro ◽  
Lucı́a Ramı́rez
Keyword(s):  
Linkage Map ◽  
Genetic Linkage ◽  
Gene Sequence ◽  
Genetic Linkage Map ◽  
Fragment Length Polymorphism ◽  
Individual Cell ◽  
Random Amplified Polymorphic Dna ◽  
Rrna Gene ◽  
Linkage Groups ◽  
Rrna Gene Sequence

ABSTRACT We have constructed a genetic linkage map of the edible basidiomycete Pleurotus ostreatus (var. Florida). The map is based on the segregation of 178 random amplified polymorphic DNA and 23 restriction fragment length polymorphism markers; four hydrophobin, two laccase, and two manganese peroxidase genes; both mating type loci; one isozyme locus (est1); the rRNA gene sequence; and a repetitive DNA sequence in a population of 80 sibling monokaryons. The map identifies 11 linkage groups corresponding to the chromosomes ofP. ostreatus, and it has a total length of 1,000.7 centimorgans (cM) with an average of 35.1 kbp/cM. The map shows a high correlation (0.76) between physical and genetic chromosome sizes. The number of crossovers observed per chromosome per individual cell is 0.89. This map covers nearly the whole genome of P. ostreatus.

scholarly journals DNA Markers from Different Linkage Regions of Watermelon Genome Useful in Differentiating among Closely Related Watermelon Genotypes

HortScience ◽  
2007 ◽  
Vol 42 (2) ◽  
pp. 210-214 ◽  
Author(s):  
Amnon Levi ◽  
Claude E. Thomas
Keyword(s):  
Linkage Map ◽  
Genetic Linkage ◽  
Genetic Linkage Map ◽  
Fragment Length Polymorphism ◽  
Citrullus Lanatus ◽  
Plant Introduction ◽  
Polymorphic Markers ◽  
Linkage Groups ◽  
Breeding Lines ◽  
Srap Markers

A genetic linkage map was previously constructed for watermelon using a wide testcross population [{Plant Accession Griffin 14113; Citrullus lanatus var. citroides (L.H. Baiely) Mansf.} × the watermelon cultivar New Hampshire Midget; NHM {(Citrullus lanatus (Thunb.) Matsum. & Nakai var. lanatus)} × United States Plant Introduction (PI) 386015 {Citrullus colocynthis (L.) Schrad.}]. One-hundred forty-six markers [randomly amplified polymorphic DNA (RAPD), intersimple sequence repeat (ISSR), amplified fragment length polymorphism (AFLP), and sequence-related amplified polymorphism (SRAP) markers] unique to NHM and representing different linkage groups on the map were tested for polymorphism among 24 watermelon cultivars limited in genetic diversity. Five (9.4%) of 53 RAPD, six (40.0%) of 15 ISSR, 30 (81.0%) of 37 AFLP, and 33 (80.5%) of 41 SRAP markers tested produced polymorphism among the 24 cultivars. The polymorphic markers used in this study are scattered throughout the watermelon genome. However, a large number (19 of the 30) of AFLP markers clustered on one linkage group on the map. The SRAP markers proved to be most effective in producing polymorphism and in representing different linkage regions of watermelon genome. The polymorphic markers represent all 10 large linkage groups and five of the nine small linkage groups (altogether 15 of 19 linkage groups) of the genetic linkage map constructed so far for watermelon. These polymorphic markers can be useful in DNA fingerprinting of cultivars, in testing seed purity of breeding lines, and in identifying triploid (seedless) hybrid watermelons derived from crosses between closely related tetraploid and diploid lines.

A genetic linkage map for Stevia rebaudiana

Genome ◽  
1999 ◽  
Vol 42 (4) ◽  
pp. 657-661 ◽  
Author(s):  
Y Yao ◽  
M Ban ◽  
J Brandle
Keyword(s):  
Linkage Map ◽  
Genetic Linkage ◽  
Rapd Markers ◽  
Genetic Linkage Map ◽  
Average Distance ◽  
Stevia Rebaudiana ◽  
Linkage Groups ◽  
Genome Map ◽  
High Level ◽  
Map Distance

To lay a foundation for molecular breeding efforts, the first genetic linkage map for Stevia rebaudiana has been constructed using segregation data from a pseudo test-cross F1 population. A total of 183 randomly amplified polymorphic DNA (RAPD) markers were analysed and assembled into 21 linkage groups covering a total distance of 1389 cM, with an average distance between markers of of 7.6 cM. The 11 largest linkage groups consisted of 4-19 loci, ranged in length from 56 to 174 cM, and accounted for 75% of the total map distance. Fifteen RAPD loci were found to be unlinked. From the 521 primers showing amplification products, 185 (35.5%) produced a total of 293 polymorphic fragments, indicating a high level of genetic diversity in stevia. Most of the RAPD markers in stevia segregated in normal Mendelian fashion.Key words: stevia, open-pollinated, genome map, RAPD.

Mapping of quantitative trait loci underlying resistance to cassava anthracnose disease

2015 ◽  
Vol 154 (7) ◽  
pp. 1209-1217 ◽  
Author(s):  
A. BOONCHANAWIWAT ◽  
S. SRAPHET ◽  
S. WHANKAEW ◽  
O. BOONSENG ◽  
D. R. SMITH ◽  
...  
Keyword(s):  
Quantitative Trait Loci ◽  
Linkage Map ◽  
Dna Markers ◽  
Quantitative Trait ◽  
Genetic Linkage ◽  
Genetic Linkage Map ◽  
Gene Annotation ◽  
Linkage Groups ◽  
Anthracnose Disease ◽  
Trait Loci

SUMMARYCassava (Manihot esculenta Crantz) is an economically important root crop in Thailand, which is ranked the world's top cassava exporting country. Production of cassava can be hampered by several pathogens and pests. Cassava anthracnose disease (CAD) is an important disease caused by the fungus Colletotrichum gloeosporioides f. sp. manihotis. The pathogen causes severe stem damage resulting in yield reductions and lack of stem cuttings available for planting. Molecular studies of cassava response to CAD will provide useful information for cassava breeders to develop new varieties with resistance to the disease. The current study aimed to identify quantitative trait loci (QTL) and DNA markers associated with resistance to CAD. A total of 200 lines of two F1 mapping populations were generated by reciprocal crosses between the varieties Huabong60 and Hanatee. The F1 samples were genotyped based on simple sequence repeat (SSR) and expressed sequence tag-SSR markers and a genetic linkage map was constructed using the JoinMap®/version3·0 program. The results showed that the map consisted of 512 marker loci distributed on 24 linkage groups with a map length of 1771·9 centimorgan (cM) and a mean interval between markers of 5·7 cM. The genetic linkage map was integrated with phenotypic data for the response to CAD infection generated by a detached leaf assay test. A total of three QTL underlying the trait were identified on three linkage groups using the MapQTL®/version4·0 program. Those DNA markers linked to the QTL that showed high statistically significant values with the CAD resistance trait were identified for gene annotation analysis and 23 candidate resistance genes to CAD infection were identified.

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