scholarly journals Chromosome-wide characterization of meiotic noncrossovers (gene conversions) in mouse hybrids

Genetics ◽  
2020 ◽  
Vol 217 (1) ◽  
pp. 1-14
Author(s):  
Vaclav Gergelits ◽  
Emil Parvanov ◽  
Petr Simecek ◽  
Jiri Forejt
Keyword(s):  
Mus Musculus ◽  
Hybrid Sterility ◽  
Wide Distribution ◽  
Mus Musculus Domesticus ◽  
Dna Double Strand Breaks ◽  
Mus Musculus Musculus ◽  
Significant Deficit ◽  
Chromosome Substitution Strains ◽  
Gene Conversions

Abstract During meiosis, the recombination-initiating DNA double-strand breaks (DSBs) are repaired by crossovers or noncrossovers (gene conversions). While crossovers are easily detectable, noncrossover identification is hampered by the small size of their converted tracts and the necessity of sequence polymorphism. We report identification and characterization of a mouse chromosome-wide set of noncrossovers by next-generation sequencing of 10 mouse intersubspecific chromosome substitution strains. Based on 94 identified noncrossovers, we determined the mean length of a conversion tract to be 32 bp. The spatial chromosome-wide distribution of noncrossovers and crossovers significantly differed, although both sets overlapped the known hotspots of PRDM9-directed histone methylation and DNA DSBs, thus supporting their origin in the standard DSB repair pathway. A significant deficit of noncrossovers descending from asymmetric DSBs proved their proposed adverse effect on meiotic recombination and pointed to sister chromatids as an alternative template for their repair. The finding has implications for the molecular mechanism of hybrid sterility in mice from crosses between closely related Mus musculus musculus and Mus musculus domesticus subspecies.

scholarly journals Chromosome-wide distribution and characterization of intersubspecific meiotic noncrossovers in mice

2019 ◽  
Author(s):  
Vaclav Gergelits ◽  
Emil Parvanov ◽  
Petr Simecek ◽  
Jiri Forejt
Keyword(s):  
Mouse Chromosome ◽  
Hybrid Sterility ◽  
Wide Distribution ◽  
Double Strand Breaks ◽  
Dna Double Strand Breaks ◽  
Strand Breaks ◽  
Dna Dsbs ◽  
Significant Deficit ◽  
Chromosome Substitution Strains

AbstractDuring meiosis, the recombination-initiating DNA double-strand breaks (DSBs) are repaired by crossovers or noncrossovers (gene conversions). While crossovers are easily detectable, the noncrossover identification is hampered by the small size of their converted tracts and the necessity of sequence polymorphism to detect them. We report identification and characterization of a mouse chromosome-wide set of noncrossovers by NGS sequencing of 10 mouse chromosome substitution strains. Based on 94 identified noncrossovers we determined the mean length of a conversion tract to be 32 basepairs. The spatial chromosome-wide distribution of noncrossovers and crossovers significantly differed, though both sets overlapped the known hotspots of PRDM9-directed histone methylation and DNA DSBs, thus proving their origin in the standard DSB repair pathway. A significant deficit of noncrossovers descending from asymmetric DSBs pointed to sister chromatids as an alternative template for their repair. The finding has implications for the molecular mechanism of hybrid sterility in mice.

Behavioral Characterization of Wild Derived Male Mice (Mus musculus musculus) of the PWD/Ph Inbred Strain: High Exploration Compared to C57BL/6J

2004 ◽  
Vol 34 (6) ◽  
pp. 621-630 ◽  
Author(s):  
Cathy Fernandes ◽  
Lin Liu ◽  
Jos� L. Paya-Cano ◽  
So?a Gregorov� ◽  
Ji?� Forejt ◽  
...  
Keyword(s):  
Inbred Strain ◽  
Mus Musculus ◽  
Male Mice ◽  
Mus Musculus Musculus ◽  
Behavioral Characterization

scholarly journals Host subspecific viral strains in European house mice: Murine cytomegalovirus in the Eastern (Mus musculus musculus) and Western house mouse (Mus musculus domesticus)

Virology ◽  
2018 ◽  
Vol 521 ◽  
pp. 92-98 ◽  
Author(s):  
Dagmar Čížková ◽  
Stuart J.E. Baird ◽  
Jana Těšíková ◽  
Sebastian Voigt ◽  
Ďureje Ľudovít ◽  
...  
Keyword(s):  
House Mouse ◽  
Mus Musculus ◽  
Murine Cytomegalovirus ◽  
House Mice ◽  
Mus Musculus Domesticus ◽  
Mus Musculus Musculus

Most classical Mus musculus domesticus laboratory mouse strains carry a Mus musculus musculus Y chromosome

Nature ◽  
1985 ◽  
Vol 315 (6014) ◽  
pp. 70-72 ◽  
Author(s):  
C. E. Bishop ◽  
P. Boursot ◽  
B. Baron ◽  
F. Bonhomme ◽  
D. Hatat
Keyword(s):  
Y Chromosome ◽  
Mus Musculus ◽  
Laboratory Mouse ◽  
Mouse Strains ◽  
Mus Musculus Domesticus ◽  
Mus Musculus Musculus

Analyse génétique de la zone d'hybridation entre les deux sous-espèces de souris Mus musculus domesticus et Mus musculus musculus en Bulgarie

Genome ◽  
1988 ◽  
Vol 30 (3) ◽  
pp. 427-437 ◽  
Author(s):  
Flavie Vanlerberghe ◽  
Pierre Boursot ◽  
Josette Catalan ◽  
Svestoslav Gerasimov ◽  
François Bonhomme ◽  
...  
Keyword(s):  
Mitochondrial Dna ◽  
Y Chromosome ◽  
Hybrid Zone ◽  
Mus Musculus ◽  
Functional Differentiation ◽  
Restriction Pattern ◽  
The Black Sea ◽  
Mus Musculus Domesticus ◽  
The Balkans ◽  
Mus Musculus Musculus

The hybrid zone between the two subspecies of mice Mus musculus domesticus and Mus musculus musculus, which has been studied extensively in Denmark, crosses Europe to the Black Sea through the Alps and the Balkans. Two hundred and seventy-nine animals were captured in 22 localities along a transect across the Balkans. The animals were characterized for seven diagnostic nuclear loci by protein electrophoresis and by restriction pattern analysis of their mitochondrial DNA. The nuclear data show a sharp transition between the two subspecies, most of the variations in allele frequencies (from 0.9 to 0.1) occurring within a 36-km section of the transect. The introgression varies from one locus to the other and is more pronounced, in terms of distance, in M. m. musculus territory. Mitochondrial DNA introgression is important but occurs in one direction only, i.e. from M. m. musculus to M. m. domesticus, while a cytoplasmic transfer from M. m. domesticus to M. m. musculus has been reported. A previous study showed that no Y chromosome introgression occurs. The different behaviour of these three types of markers could be due to the interaction between selection against hybrid genomes and meiotic recombination. Objectively, it would appear that the genes that can introgress are neutral or nearly so and have been separated from deleterious genes they were linked to by recombination. This could explain the differential introgression between autosomal loci. The mitochondrial and Y chromosomes undergo no or very little recombination and each is transmitted as a whole. Their degree of introgression is thus indicative of the intensity of selection resulting from the amount of functional differentiation between the two taxa, which seems to be strong for the Y chromosome and weak for mitochondrial DNA. We propose that the asymmetry of nuclear introgression is due to different population structures. As M. m. musculus is relatively less structured, the rapid spreading of introgressed genes would be favoured. Such a scheme, however, can hardly account for the unidirectionality of the mitochondrial flow, which could be due to sex-dependant behaviour.Key words: mice, hybrid zone, introgression, enzyme polymorphism.

scholarly journals Mitochondrial DNA in the hybrid zone between Mus musculus musculus and Mus musculus domesticus: a comparison of two transects

2005 ◽  
Vol 84 (3) ◽  
pp. 363-378 ◽  
Author(s):  
EVA BOŽÍKOVÁ ◽  
PAVEL MUNCLINGER ◽  
KATHERINE C. TEETER ◽  
PRISCILLA K. TUCKER ◽  
MILOŠ MACHOLÁN ◽  
...  
Keyword(s):  
Mitochondrial Dna ◽  
Hybrid Zone ◽  
Mus Musculus ◽  
Mus Musculus Domesticus ◽  
Mus Musculus Musculus

Experimental evidence of genetic determinism in high susceptibility to intestinal pinworm infection in mice: a hybrid zone model

Parasitology ◽  
1993 ◽  
Vol 106 (4) ◽  
pp. 387-393 ◽  
Author(s):  
C. Moulia ◽  
N. Le Brun ◽  
J. Dallas ◽  
A. Orth ◽  
F. Renaud
Keyword(s):  
Hybrid Zone ◽  
Mus Musculus ◽  
Genetic Parameters ◽  
Zone Model ◽  
Helminth Parasites ◽  
Mus Musculus Domesticus ◽  
High Susceptibility ◽  
Mus Musculus Musculus ◽  
Aspiculuris Tetraptera ◽  
Genetically Determined

SUMMARYIn the hybrid zone of the two mouse subspecies Mus musculus musculus and Mus musculus domesticus, mice with hybrid genotypes harbour, on the average, more helminth parasites (cestodes and nematodes) than mice of the two parental taxa. In order to determine the roles played by genetic parameters in this phenomenon, mice with recombined and parental genotypes were experimentally infected with the intestinal pinworm Aspiculuris tetraptera, a natural parasite of the house mouse. The results showed that the high susceptibility of the hybrid zone mice is genetically determined. In addition, this study shows the occurrence of variability among resistant parental populations.

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