Purification and characterization of 9-O-acetylated sialoglycoproteins from leukemic cells and their potential as immunological tool for monitoring childhood acute lymphoblastic leukemia

L-asparaginase from bacteria has been used in treatment of acute lymphoblastic leukemia. The aim of this study was to purify and characterize L-asparaginase fromPhaseolus vulgarisseeds instead of microbial sources. L-asparaginase was purified to apparent homogeneity. The enzyme has molecular mass of 79 kDa. The purified asparaginase had very low activity toward a number of asparagine and glutamine analogues. L-asparaginase was free from glutaminase activity. Kinetic parameters, Km andVmax of purified enzyme, were found to be 6.72 mM and 0.16 μM, respectively. The enzyme had optimum pH at 8.0. The enzyme showed high stability at alkaline pH (pH 7.5–9.0) when incubated for up to 24 h. L-asparaginase had the same temperature optimum and thermal stability at 37°C. K+was able to greatly enhance the activity of asparaginase by 150% compared with other metals tested. In conclusion, L-asparaginase showed no glutaminase activity and good stability over a wide range of physiological conditions, and thus it could be used as a potential candidate for treatment of acute lymphoblastic leukemia.

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PARP-1 expression in CD34+ leukemic cells in childhood acute lymphoblastic leukemia: relation to response to initial therapy and other prognostic factors

Polish Journal of Pathology ◽

10.5114/pjp.2015.54957 ◽

2015 ◽

Vol 3 ◽

pp. 239-245 ◽

Cited By ~ 4

Author(s):

Agnieszka Kruk ◽

Tomasz Ociepa ◽

Tomasz Urasiński ◽

Jerzy Grabarek ◽

Elżbieta Urasińska

Keyword(s):

Prognostic Factors ◽

Acute Lymphoblastic Leukemia ◽

Lymphoblastic Leukemia ◽

Initial Therapy ◽

Childhood Acute Lymphoblastic Leukemia ◽

Leukemic Cells ◽

Parp 1

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Application of Fluorescence In Situ Hybridization on Cerebrospinal Fluid Cytospins for the Detection of Residual Leukemic Cells in Patients With Childhood Acute Lymphoblastic Leukemia

American Journal of Clinical Pathology ◽

10.1093/ajcp/aqy160 ◽

2018 ◽

Vol 151 (4) ◽

pp. 416-423

Author(s):

Sang Mee Hwang ◽

Hee Sue Park ◽

Seungman Park ◽

Sung-Min Kim ◽

Kyung Taek Hong ◽

...

Keyword(s):

Cerebrospinal Fluid ◽

In Situ Hybridization ◽

Acute Lymphoblastic Leukemia ◽

Fluorescence In Situ Hybridization ◽

Lymphoblastic Leukemia ◽

Childhood Acute Lymphoblastic Leukemia ◽

Leukemic Cells

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Characterization of high-hyperdiploidy in childhood acute lymphoblastic leukemia with gain of a single chromosome 21

Leukemia & Lymphoma ◽

10.1080/10428190701656110 ◽

2007 ◽

Vol 48 (12) ◽

pp. 2457-2460 ◽

Cited By ~ 1

Author(s):

Angela Brown ◽

Felix Niggli ◽

Heinz Hengartner ◽

Ueli Caflisch ◽

Luisa Nobile ◽

...

Keyword(s):

Acute Lymphoblastic Leukemia ◽

Lymphoblastic Leukemia ◽

Chromosome 21 ◽

Childhood Acute Lymphoblastic Leukemia ◽

Single Chromosome

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Characterization of acute undifferentiated leukemia by combined analysis of plasma membrane-associated gamma-glutamyltranspeptidase and soluble terminal transferase

Blood ◽

10.1182/blood.v66.2.255.255 ◽

1985 ◽

Vol 66 (2) ◽

pp. 255-258 ◽

Cited By ~ 4

Author(s):

D Heumann ◽

G Losa ◽

C Barras ◽

A Morell ◽

V von Fliedner

Keyword(s):

Plasma Membrane ◽

Acute Lymphoblastic Leukemia ◽

Enzyme Activities ◽

Colorimetric Assay ◽

Lymphoblastic Leukemia ◽

Leukemic Cells ◽

Acute Leukemias ◽

Combined Analysis ◽

Enzyme Markers

Abstract gamma-Glutamyltranspeptidase (gamma-GT) is a plasma membrane-associated enzyme present in blasts of certain acute leukemias. We analyzed 90 cases of undifferentiated and differentiated acute leukemias for gamma- GT, using a colorimetric assay. Blasts of all patients with common acute lymphoblastic leukemia (ALL) and T-ALL were negative for gamma-GT (less than 5 units). In contrast, gamma-GT was significantly elevated in acute myeloblastic or monoblastic leukemia blasts (P less than .001). In 16 cases of acute undifferentiated leukemia (AUL) studied, the levels of gamma-GT ranged from 0 to 93 units; in eight cases, gamma- GT was positive (greater than 5 units), and six of these had 2% to 5% Sudan black-positive leukemic cells in the blast-enriched suspension. Combined gamma-GT/TdT analysis revealed that both enzyme markers were mutually exclusive in 75% of AUL cases, suggesting that gamma-GT+/TdT- blasts are of nonlymphoid origin, and gamma-GT-/TdT+ blasts are of lymphoid origin. Two cases were devoid of both enzyme activities and could represent truly undifferentiated leukemia. Thus, combined gamma- GT/TdT analysis underlines the heterogeneity of AUL and appears to be useful in defining the lineage commitment of undifferentiated leukemic blasts.

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