scholarly journals Site-specific glycoproteomics confirms that protein structure dictates formation of N-glycan type, core fucosylation and branching

Glycobiology ◽  
2012 ◽  
Vol 22 (11) ◽  
pp. 1440-1452 ◽  
Author(s):  
Morten Thaysen-Andersen ◽  
Nicolle H Packer
Keyword(s):  
Protein Structure ◽  
Site Specific ◽  
Core Fucosylation ◽  
Glycan Type

scholarly journals Cross-species comparison of site-specific evolutionary-rate variation in influenza haemagglutinin

2013 ◽  
Vol 368 (1614) ◽  
pp. 20120334 ◽  
Author(s):  
Austin G. Meyer ◽  
Eric T. Dawson ◽  
Claus O. Wilke
Keyword(s):  
Protein Structure ◽  
Sialic Acid ◽  
Avian Influenza ◽  
Evolutionary Rate ◽  
Solvent Accessibility ◽  
Rate Variation ◽  
Structural Constraints ◽  
Binding Region ◽  
Site Specific ◽  
Sialic Acid Binding

We investigate the causes of site-specific evolutionary-rate variation in influenza haemagglutinin (HA) between human and avian influenza, for subtypes H1, H3, and H5. By calculating the evolutionary-rate ratio, ω = d N /d S as a function of a residue's solvent accessibility in the three-dimensional protein structure, we show that solvent accessibility has a significant but relatively modest effect on site-specific rate variation. By comparing rates within HA subtypes among host species, we derive an upper limit to the amount of variation that can be explained by structural constraints of any kind. Protein structure explains only 20–40% of the variation in ω . Finally, by comparing ω at sites near the sialic-acid-binding region to ω at other sites, we show that ω near the sialic-acid-binding region is significantly elevated in both human and avian influenza, with the exception of avian H5. We conclude that protein structure, HA subtype, and host biology all impose distinct selection pressures on sites in influenza HA.

scholarly journals Label-free relative quantification of alpha-2-macroglobulin site-specific core-fucosylation in pancreatic cancer by LC-MS/MS

2013 ◽  
pp. n/a-n/a ◽  
Author(s):  
Zhenxin Lin ◽  
Haidi Yin ◽  
Andy Lo ◽  
Mack T. Ruffin ◽  
Michelle A. Anderson ◽  
...  
Keyword(s):  
Pancreatic Cancer ◽  
Relative Quantification ◽  
Label Free ◽  
Site Specific ◽  
Free Relative ◽  
Core Fucosylation

Site-Specific Interrogation of Protein Structure and Stability

2021 ◽  
pp. 65-87
Author(s):  
Debopreeti Mukherjee ◽  
Ismail A. Ahmed ◽  
Feng Gai
Keyword(s):  
Protein Structure ◽  
Site Specific ◽  
Structure And Stability
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