An endoglycosidase-assisted LC-MS/MS-based strategy for the analysis of site-specific core-fucosylation of low-concentrated glycoproteins in human serum using prostate-specific antigen (PSA) as example

2018 ◽  
Vol 480 ◽  
pp. 1-8 ◽  
Author(s):  
Robert Lang ◽  
Andreas Leinenbach ◽  
Johann Karl ◽  
Magdalena Swiatek-de Lange ◽  
Uwe Kobold ◽  
...  
Keyword(s):  
Human Serum ◽  
Prostate Specific Antigen ◽  
Specific Antigen ◽  
Site Specific ◽  
Core Fucosylation

Measurement of total and free prostate specific antigen (PSA) in human serum samples using an ultra-microanalytical system

2021 ◽  
pp. 114470
Author(s):  
Juliette M. Cazanave Mora ◽  
Ruben del Valle García ◽  
Lilian Pérez López ◽  
Dunia C. Bequer Ariza ◽  
Orlando Zulueta Rodríguez ◽  
...  
Keyword(s):  
Human Serum ◽  
Prostate Specific Antigen ◽  
Specific Antigen ◽  
Serum Samples ◽  
Human Serum Samples

Human serum albumin as an intrinsic signal amplification amplifier for ultrasensitive assays of the prostate-specific antigen in human plasma

2020 ◽  
Vol 56 (12) ◽  
pp. 1843-1846 ◽  
Author(s):  
Le Yang ◽  
Jing Zheng ◽  
Zhen Zou ◽  
Haiyan Cai ◽  
Peng Qi ◽  
...  
Keyword(s):  
Human Serum Albumin ◽  
Serum Albumin ◽  
Human Serum ◽  
Human Plasma ◽  
Prostate Specific Antigen ◽  
Signal Amplification ◽  
Specific Antigen ◽  
Disease Biomarkers ◽  
Intrinsic Signal ◽  
Blood Tests

Endogenous human serum albumin is used as an intrinsic signal amplification amplifier for ultrasensitive assays of disease biomarkers in blood tests.

scholarly journals Two-dimensional gel electrophoresis detects prostate-specific antigen–α1-antichymotrypsin complex in serum but not in prostatic fluid

1997 ◽  
Vol 43 (2) ◽  
pp. 352-359 ◽  
Author(s):  
Yi Qian ◽  
Julia A Sensibar ◽  
David J Zelner ◽  
Anthony J Schaeffer ◽  
Judith A Finlay ◽  
...  
Keyword(s):  
Western Blot ◽  
Human Serum ◽  
Prostate Specific Antigen ◽  
Seminal Plasma ◽  
Gel Electrophoresis ◽  
Specific Antigen ◽  
Two Dimensional ◽  
Two Dimensional Gel Electrophoresis ◽  
Prostatic Fluid ◽  
Three Body

Abstract We investigated the interaction between prostate-specific antigen (PSA) and 1-antichymotrypsin (ACT) in prostatic secretions, identifying PSA and ACT in human serum, prostatic fluid, and seminal plasma by two-dimensional gel electrophoresis (2-D PAGE). Both PSA and ACT were detected in all three body fluids, but PSA-ACT complex was detected only in serum. Moreover, the 2-D PAGE Western blot staining profile for ACT from serum differed from that for prostatic fluid or seminal plasma. Incubation of prostatic fluid with purified ACT led to formation of PSA-ACT complex. Incubation of prostatic fluid with purified PSA, however, failed to form the complex, suggesting that the ACT in prostatic fluid was inactive or inhibited. Given that physiological concentrations of zinc inhibited the formation of PSA-ACT complex, we consider zinc a possible physiological inhibitor of the formation of the PSA-ACT complex. These results indicate that the failure to detect the PSA-ACT complex in prostatic fluid could be related to the inactivation of ACT, the presence of inhibitors (e.g., zinc), or simply the PSA:ACT ratio in the fluid.

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