scholarly journals P-086. Influence of sperm selection methods on recovery rate of chromatin-condensed (chromomycin A3-staining), morphologically normal spermatozoa and IVF-ET outcome

1999 ◽  
Vol 14 (Suppl_3) ◽  
pp. 183-184
Author(s):  
M.E. Hammadeh ◽  
A. Kühnen ◽  
P. Rosenbaum ◽  
T. Georg ◽  
W. Schmidt
Keyword(s):  
Recovery Rate ◽  
Sperm Selection ◽  
Selection Methods ◽  
Chromomycin A3 ◽  
Ivf Et

scholarly journals JUST KEEP SWIMMING: COMPARING PGT OUTCOMES IN IVF-ICSI CYCLES USING VARYING SPERM SELECTION METHODS

2021 ◽  
Vol 116 (1) ◽  
pp. e18-e19
Author(s):  
Patel S ◽  
Lynn R ◽  
Vitale K ◽  
Pechansky C ◽  
Usmani S ◽  
...  
Keyword(s):  
Sperm Selection ◽  
Selection Methods

scholarly journals Isolate® and Optiprep® minigradients as alternatives for sperm selection in bovine in vitro embryo production

2014 ◽  
Vol 94 (1) ◽  
pp. 35-42 ◽  
Author(s):  
L. L. Vianna ◽  
J. Pradieé ◽  
E. C. S. Santos ◽  
A. O. Gonçalves ◽  
L. F. M. Pfeifer ◽  
...  
Keyword(s):  
Sex Ratio ◽  
Sperm Motility ◽  
Semen Quality ◽  
Membrane Integrity ◽  
Sperm Selection ◽  
Selection Methods ◽  
Embryo Production ◽  
Embryo Survival ◽  
In Vitro Embryo Production

Vianna, L. L., Pradieé, J., Santos, E. C. S., Gonçalves, A. O., Pfeifer, L. F. M., Rheingantz, M. G. T., Dode, M. A. N., Vieira, A. D., Lima, V. F. H., Correa, M. N. and Pegoraro, L. M. C. 2014. Isolate® and Optiprep® minigradients as alternatives for sperm selection in bovine in vitro embryo production. Can. J. Anim. Sci. 94: 35–42. The objective of this study was to evaluate alternatives in small volumes to conventional gradient of Percoll® on semen quality, in vitro embryo production, sex ratio and embryo survival after vitrification. Thawed semen was randomly allocated to one of four density gradient selection methods: (1) conventional Percoll® (P), (2) MiniPercoll (MP), (3) MiniIsolate (MI), and (4) MiniOptiprep (MO). Sperm kinetics and quality were evaluated. Use of P, MP and MI gradients did not affect sperm motility (P>0.05). However, there was a decrease in total and progressive sperm motility in MO (70.8 and 51.3% vs. 87.3 and 69.5% for P; 87.3 and 73% for MP; 92.3 and 78.8% for MI; P<0.05). The MO had lower membrane integrity compared with P, MP and MI (39.7 vs. 70.5, 72.3, 63.8%, respectively, P<0.05). The percentage of blastocysts produced was higher in MI than in MP and MO (21.1 vs. 16.1 and 16.9%, P<0.05) and similar to P (18.4%; P>0.05). Sex ratio and embryo survival after vitrification were similar among groups (P>0.05). Semen selected by Isolate and Optiprep gradient, at the concentrations and small volumes used, demonstrated similar characteristics and in vitro embryo production to conventional Percoll® gradient.

scholarly journals Mini-percoll gradient may be used for frozen-thawed sperm selection in sheep

2019 ◽  
Vol 71 (2) ◽  
pp. 455-463 ◽  
Author(s):  
C.C.S. Olivares ◽  
J.M.G. Souza-Fabjan ◽  
J.F. Fonseca ◽  
H.F.R.A. Saraiva ◽  
L.R. Côrtes ◽  
...  
Keyword(s):  
Plasma Membrane ◽  
Centrifugal Force ◽  
Recovery Rate ◽  
Handling Time ◽  
Sperm Parameters ◽  
Sperm Selection ◽  
Percoll Gradient ◽  
Ram Sperm ◽  
First Time ◽  
Motility Parameters

ABSTRACT This study evaluated the effect of increasing centrifugal force and reducing centrifugation time and volume in Percoll protocols on ram sperm parameters. Commercial semen of Santa Inês rams were used and five treatments were performed: traditional Percoll and mini-Percoll (MP) techniques (I- 5000 x g, 5min; II- 2500 x g, 5min; III- 1250 x g, 5min; IV- 700 x g, 10min). At post-thawing (PT) and post-selection protocols (0h), samples were assessed for spermatozoa recovery rate, motility, plasma membrane (PM) integrity, sperm capacitation and morphology and incubated at 37 C for 1, 2 and 3h. The sperm recovery rate averaged 9.1±1.4%, and most motility parameters were similar (P> 0.05) among protocols. VCL (µm/s) was higher (P< 0.05) after MP-II, III and IV (66.1±4.5) than traditional Percoll (46.3±4.9). Capacitation status and PM integrity were similar (P> 0.05) among treatments. For the first time, we have demonstrated the reduction of the gradient volume and centrifugation time associated with an increase on centrifugation force at Percoll can be successfully used for frozen-thawed ram sperm selection. MP may be used instead of traditional Percoll, decreasing costs and semen handling time.

scholarly journals Use of a colloid to optimize centrifugation in the selection of bovine sperm for IVF

2018 ◽  
Vol 39 (4) ◽  
pp. 1607 ◽  
Author(s):  
Cibele Garcia Moreira Gonçalves ◽  
Fábio Gallas Leivas ◽  
Daniele Missio ◽  
Francielli Weber Santos ◽  
Eduardo Brum Schwengber ◽  
...  
Keyword(s):  
Recovery Rate ◽  
Sperm Quality ◽  
Ros Production ◽  
Sperm Selection ◽  
Percoll Gradient ◽  
Bovine Embryos ◽  
Cleavage Rate ◽  
The Impact ◽  
Normal Fertilization

This study aimed to evaluate the effect of the force and duration of centrifugation and the impact of cushioned centrifugation on sperm selection by Percoll gradient, on sperm quality and development kinetics of in vitro produced bovine embryos. Two experiments were performed. In Experiment I, a pool of semen was selected by Percoll gradients and the pellet was divided into four groups and distributed in a 2 × 2 factorial, with two forces (2200 × g or 9000 × g) and two durations (1 min or 3 min) of centrifugation. In Experiment II, semen was divided into two groups and selected by Percoll gradient with Cushion Fluid (CF) or without CF (Control) in the second centrifugation. The morphofunctionality, biochemical characteristics and fertilizing capacity of the selected sperms were evaluated. In addition, the development of the resulting bovine embryos was monitored for 48 h post-insemination. Duncan and Chi-square tests (P < 0.05) were used to compare the means. In Experiment I, there was a significant increase in sperm vigor (P < 0.05) after sperm selection in all treatments. The force and duration of centrifugation did not have any effect on sperm motility, vigor, and recovery rate among the different treatments (P > 0.05). In Experiment II, the recovery rate and reactive oxygen species (ROS) production in semen were similar among treatments (P > 0.05) although a higher ROS production was observed in the CF fertilization medium. Total fertilization rate was superior in the CF group (65.4 ± 5.3%) compared to that in Control (39.6 ± 4.9%). However, the normal fertilization and cleavage rate did not differ between the Control (94 ± 6.3% and 58.3 ± 8.3%) and CF (89 ± 7.1% and 75.0 ± 7.3%) groups. The reduction in the force and duration of centrifugation did not decrease the sperm recovery during selection by the Percoll gradient and the use of CF in the second centrifugation did not affect the normal fertilization and development of bovine IVF embryos up to 48 h.

Sperm selection methods in IVF programs (literature review)

2019 ◽  
Vol 25 (2) ◽  
pp. 83
Author(s):  
R. V. Nazarenko ◽  
V. M. Zdanovskiy
Keyword(s):  
Literature Review ◽  
Sperm Selection ◽  
Selection Methods

scholarly journals Successful Pregnancy in a Couple with Severe Male Factor Infertility after Selection of Sperm with Cytoplasmic Droplets

2015 ◽  
Vol 2015 ◽  
pp. 1-4 ◽  
Author(s):  
Jenna Bellish ◽  
David H. McCulloh ◽  
Khaliq Ahmad ◽  
Peter G. McGovern
Keyword(s):  
Dna Fragmentation ◽  
Embryo Development ◽  
Male Partner ◽  
Male Factor Infertility ◽  
Sperm Selection ◽  
Selection Methods ◽  
Male Factor ◽  
Successful Pregnancy ◽  
Live Births ◽  
Selection Of

We present live births resulting from two separate IVF cycles in a couple in which ICSI was performed with sperm specifically selected for presence of small cytoplasmic droplets. These cycles followed previous cycles using standard sperm selection methods in which very poor embryo development and no pregnancies ensued. The male partner was diagnosed with severe male factor infertility including elevated DNA fragmentation.

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