695Gene and environmental factors for liver fibrosis among Japanese general population with NAFLD

Abstract Background A part of nonalcoholic fatty liver disease (NAFLD) develops nonalcoholic steatohepatitis (NASH), liver cirrhosis and hepatocellular carcinoma. Metabolic syndrome is involved in their development, and hepatic fibrosis plays an important role in their pathogenesis. Patatin-like phospholipase domain-containing protein 3 (PNPLA3) gene encode proteins on hepatic lipid metabolism regulation, and PNPLA3 gene polymorphism is associated with the development and progression of NAFLD. To investigate the gene and environmental factors, and their interaction for liver fibrosis, we conducted a prospective study among Japanese general population with NAFLD. Methods The subjects were 295 general residents who participated as a part of the Japan Multi-Institutional Collaborative Cohort (J-MICC) study in Kagoshima, Japan, and were followed for 5 years. NAFLD was defined as fatty liver using abdominal ultrasonography. PNPLA3 C/G polymorphism (rs738409) was analysed using TaqMan PCR. Hepatic fibrosis was defined using FIB 4 index (≥1.3). Logistic regression model was used to estimate the odds ratios (ORs) after adjusted for confounding factors. Results Mean age was 55.1 years and liver fibrosis was observed at 73.2% among NAFLD subjects. No environmental factors were associated with liver fibrosis, and PNPLA3 GG genotype was not associated with liver fibrosis, too. However, the ORs of CC & CG, and GG genotypes for dyslipidemia was respectively 0.45 (0.19-1.04) and 11.9 (1.81-78.4), and their interaction was significant (p = 0.01). Conclusions The interaction between PNPLA3 GG gene polymorphism and dyslipidemia for liver fibrosis was observed. Key messages Genetic susceptibility with dyslipidemia may be involved in liver fibrosis of NAFLD.

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Advancement of hepatic fibrosis among general women with/without NAFLD

European Journal of Public Health ◽

10.1093/eurpub/ckz187.162 ◽

2019 ◽

Vol 29 (Supplement_4) ◽

Author(s):

R Ibusuki ◽

I Shimoshikiryo ◽

K Shimatani ◽

D Nishimoto ◽

S Maenohara ◽

...

Keyword(s):

Hepatocellular Carcinoma ◽

Liver Cirrhosis ◽

General Population ◽

Fatty Liver ◽

Hepatic Fibrosis ◽

Type Iv Collagen ◽

Related Factors ◽

Abdominal Ultrasonography ◽

Type Iv ◽

Type Iv Collagen 7S

Abstract Background Nonalcoholic fatty liver disease (NAFLD) is increasing, being prevalent at 30% of the general population worldwide. A part of NAFLD develops nonalcoholic steatohepatitis (NASH), liver cirrhosis and hepatocellular carcinoma. Hepatic fibrosis plays an important role in their pathogenesis. However, it is unclear how hepatic fibrosis is observed and advanced in NAFLD among general population. To investigate the hepatic fibrosis among general population, we prospectively observed hepatic fibrosis using serum markers. Methods The subjects were 228 women who participated as a part of the Japan Multi-Institutional Collaborative Cohort (J-MICC) study in Kagoshima, Japan, and were followed from 2005 to 2014. NAFLD was defined as fatty liver by abdominal ultrasonography; consuming ethanol < 20 g/day; and being none hepatitis B and C carriers. NAFLD were confirmed after two-time consecutive examination, because its disappearance is ambiguous in some cases. Hepatic fibrosis was evaluated using serum M2BPGi and Type IV collagen 7s. The comparison of their changed values between groups was done using the ANOVA adjusted for age. The association between their change and related factors was done using general linear regression model. Results The prevalence of NAFLD was 31.6% at baseline. In the 5-year observation, the NAFLD + => NAFLD + ( ++) group was 23.7%; − +, 2.6%; + −, 7.9%; and - - (control), 65.8%. The values of M2BPGi and Type IV collagen 7s were higher in ++, -+, +- groups than controls at baseline. The change of M2BPGi values was observed in all groups, including controls, and the changed values were higher in ++ and -+ groups. Higher creatinine levels were positively associated with change of M2BPGi values. In contrast, the change of Type IV collagen 7s was not apparent. Conclusions This study suggested hepatic fibrosis was advanced with age among general women without NAFLD, and the presence of NAFLD enhanced hepatic fibrosis more. Key messages Hepatic fibrosis may be slightly developing with age among general population, and will be enhanced with fatty liver. It is important to prevent fatty liver development to control risk factors, such as obesity and metabolic syndrome, to reduce the risk of NASH, liver cirrhosis and hepatocellular carcinoma.

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P068 Case finding for hepatic fibrosis in at-risk populations – enhanced liver fibrosis score detects more in alcohol than nonalcoholic fatty liver disease

10.1136/gutjnl-2021-basl.77 ◽

2021 ◽

Author(s):

Alex Hinkson ◽

Jacob Feathers ◽

Jason Punnamkuzhy ◽

Firuza Dzhakhangirli ◽

Ian AC Rowe ◽

...

Keyword(s):

Liver Disease ◽

Liver Fibrosis ◽

Fatty Liver ◽

Nonalcoholic Fatty Liver Disease ◽

Hepatic Fibrosis ◽

Fatty Liver Disease ◽

Case Finding ◽

Fibrosis Score ◽

Nonalcoholic Fatty Liver ◽

At Risk Populations

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Enhanced liver fibrosis (ELF) score accurately detects advanced fibrosis in nonalcoholic fatty liver disease (NAFLD)

10.1055/s-0038-1654642 ◽

2018 ◽

Author(s):

K Staufer ◽

R Marculescu ◽

B Obermayer-Pietsch ◽

J Stift ◽

C Lackner ◽

...

Keyword(s):

Liver Disease ◽

Liver Fibrosis ◽

Fatty Liver ◽

Nonalcoholic Fatty Liver Disease ◽

Fatty Liver Disease ◽

Advanced Fibrosis ◽

Nonalcoholic Fatty Liver

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Association between the frequency of daily tooth brushing and development of nonalcoholic fatty liver disease

Digestive Diseases ◽

10.1159/000514930 ◽

2021 ◽

Author(s):

Kazuki Yamamoto ◽

Takashi Ikeya ◽

Shuhei Okuyama ◽

Katsuyuki Fukuda ◽

Daiki Kobayashi

Keyword(s):

Liver Disease ◽

Fatty Liver ◽

Nonalcoholic Fatty Liver Disease ◽

Fatty Liver Disease ◽

Primary Outcome ◽

Tooth Brushing ◽

Health Checkup ◽

Abdominal Ultrasonography ◽

Nonalcoholic Fatty Liver ◽

The Mean

Background & Aims: This study aimed to evaluate the association between the frequency of daily tooth brushing and the development of nonalcoholic fatty liver disease (NAFLD). Methods: A retrospective longitudinal study was conducted from 2005 to 2012 at the Center for Preventive Medicine at St. Luke's International Hospital, Japan. Data on all participants who underwent a health checkup during the study period were collected. NAFLD was diagnosed by abdominal ultrasonography, and all participants who were diagnosed with NALFD at the time of their initial visit, consumed alcohol in any amount, or had received only one health checkup were excluded. The questionnaire for the frequency of daily tooth brushing was conducted as part of health checkups. The primary outcome was the risk of developing NAFLD according to the frequency of daily tooth brushing (1-2 times a day, or 3 times a day) compared to those who brush teeth once or less than once a day. Results: Data were collected from 25,804 people. A total of 3,289 (12.7%) participants developed NAFLD. The mean age was 45.2 years, and 6,901 (26.7%) of the participants were male. The risk of developing NAFLD significantly decreased with increased frequency of daily tooth brushing. Adjusted odds ratios (ORs) are as follows: Brushing teeth 1-2 times a day (OR: 0.85, 95% CI: 0.77-0.95), and 3 times a day (OR: 0.74, 95% CI: 0.67-0.82). Conclusions: Frequent tooth brushing was shown to significantly reduce the risk of developing NAFLD.　

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Prediction of Nonalcoholic Fatty Liver Disease Using Noninvasive and Non-Imaging Procedures in Japanese Health Checkup Examinees

Diagnostics ◽

10.3390/diagnostics11010132 ◽

2021 ◽

Vol 11 (1) ◽

pp. 132

Author(s):

Kenichiro Murayama ◽

Michiaki Okada ◽

Kenichi Tanaka ◽

Chika Inadomi ◽

Wataru Yoshioka ◽

...

Keyword(s):

Liver Disease ◽

Liver Fibrosis ◽

Fatty Liver ◽

Japanese Population ◽

Fatty Liver Disease ◽

Health Checkup ◽

Nonalcoholic Fatty Liver ◽

General Japanese Population ◽

Advanced Liver Fibrosis ◽

Zju Index

Access to imaging is limited for diagnosing nonalcoholic fatty liver disease (NAFLD) in general populations. This study evaluated the diagnostic performance of noninvasive and nonimaging indexes to predict NAFLD in the general Japanese population. Health checkup examinees without hepatitis virus infection or habitual alcohol drinking were included. Fatty liver was diagnosed by ultrasonography. The hepatic steatosis index (HSI), Zhejiang University (ZJU) index, and fatty liver index (FLI) were determined, and risk of advanced liver fibrosis was evaluated by the fibrosis-4 index. NAFLD was diagnosed in 1935 (28.0%) of the 6927 subjects. The area under the receiver operating characteristic (AUROC) curve of the HSI, ZJU index, and FLI was 0.874, 0.886, and 0.884, respectively. The AUROC of the ZJU index (p < 0.001) and FLI (p = 0.002) was significantly greater than that for the HSI. In subjects with a high risk of advanced fibrosis, the sensitivity of the HSI, ZJU index, and FLI were 88.8%, 94.4%, and 83.3% with a low cut-off value and the specificity was 98.5%, 100%, and 100% with a high cut-off value. In conclusion, all indexes were useful to diagnose NAFLD in the general Japanese population and in subjects with potentially advanced liver fibrosis.

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