Toxic Activity of Purified Lipopolysaccharide of Neisseria gonorrhoeae for Human Fallopian Tube Mucosa

1981 ◽  
Vol 143 (3) ◽  
pp. 432-439 ◽  
Author(s):  
C. R. Gregg ◽  
M. A. Melly ◽  
C. G. Hellerqvist ◽  
J. G. Coniglio ◽  
Z. A. McGee
Keyword(s):  
Neisseria Gonorrhoeae ◽  
Fallopian Tube ◽  
Toxic Activity ◽  
Human Fallopian Tube

scholarly journals Differential intracellular efficacies of ciprofloxacin and cefixime against Neisseria gonorrhoeae in human fallopian tube organ culture.

1997 ◽  
Vol 41 (7) ◽  
pp. 1547-1551 ◽  
Author(s):  
J P Phanucharas ◽  
G L Gorby
Keyword(s):  
Epithelial Cells ◽  
Confidence Interval ◽  
Neisseria Gonorrhoeae ◽  
Organ Culture ◽  
Fallopian Tube ◽  
Cytochalasin D ◽  
Human Fallopian Tube ◽  
Culture Model ◽  
Better Than

This study compared the abilities of ciprofloxacin and cefixime to kill intracellular Neisseria gonorrhoeae in a human fallopian tube organ culture assay. When invasion was inhibited by cytochalasin D, 0.996% of the tissue-associated gonococci survived ciprofloxacin exposure compared to 1.70% of gonococci exposed to cefixime (95% confidence interval for the ratio of the means, 0.267 to 1.30), indicating that the two antibiotics did not significantly differ in the ability to kill extracellular attached organisms. In the absence of cytochalasin D, 1.63% survived ciprofloxacin exposure while 9.76% survived cefixime treatment (95% confidence interval for the ratio of the means, 0.067 to 0.418). These results suggest that ciprofloxacin penetrated epithelial cells and killed intracellular gonococci better than did cefixime. Thus, at concentrations achievable in serum, ciprofloxacin was more effective in total gonococcal killing than cefixime in this human fallopian tube organ culture model.

scholarly journals Neisseria gonorrhoeae Mutants Altered in Toxicity to Human Fallopian Tubes and Molecular Characterization of the Genetic Locus Involved

1999 ◽  
Vol 67 (2) ◽  
pp. 643-652 ◽  
Author(s):  
Cindy Grove Arvidson ◽  
Risa Kirkpatrick ◽  
Manon T. Witkamp ◽  
Jason A. Larson ◽  
Christel A. Schipper ◽  
...  
Keyword(s):  
Neisseria Gonorrhoeae ◽  
Fallopian Tube ◽  
Signal Sequence ◽  
Genetic Locus ◽  
Open Reading Frames ◽  
Fallopian Tubes ◽  
Bacterial Survival ◽  
Wild Type ◽  
Human Fallopian Tube ◽  
Wild Type Parent

ABSTRACT In an effort to identify potential cytotoxins expressed byNeisseria gonorrhoeae, we have identified a locus that, when mutated in the gonococcus, results in a significant increase in toxicity of the strain to human fallopian tube organ cultures (HFTOC). This locus, gly1, contains two open reading frames (ORFs) which are likely cotranscribed. ORF1 encodes a polypeptide of 17.8 kDa with a signal sequence that is recognized and processed inEscherichia coli and N. gonorrhoeae. The 15.6-kDa processed polypeptide has been observed in membrane fractions and filtered spent media from cultures of E. coli expressing gly1 and in outer membrane preparations of wild-type N. gonorrhoeae. The gly1 locus is not essential for bacterial survival, and it does not play a detectable role in epithelial cell adhesion, invasion, or intracellular survival. However, agly1 null mutant causes much more damage to fallopian tube tissues than its isogenic wild-type parent. A strain complemented intrans for the gly1 mutation showed a level of toxicity to HFTOC similar to the level elicited by the wild-type parent. Taken together, these results indicate an involvement of the gly1 locus in the toxicity of N. gonorrhoeae to human fallopian tubes.

scholarly journals Digital confocal microscopy allows measurement and three-dimensional multiple spectral reconstruction of Neisseria gonorrhoeae/epithelial cell interactions in the human fallopian tube organ culture model.

1994 ◽  
Vol 42 (3) ◽  
pp. 297-306 ◽  
Author(s):  
G L Gorby
Keyword(s):  
Image Analysis ◽  
Confocal Microscopy ◽  
Neisseria Gonorrhoeae ◽  
Organ Culture ◽  
Fallopian Tube ◽  
Three Dimensional ◽  
Target Tissue ◽  
Cell Nuclei ◽  
Computerized Image Analysis ◽  
Human Fallopian Tube

A strategy for measuring Neisseria gonorrhoeae attachment and invasion in the human Fallopian tube organ culture (FTOC) model via computerized image analysis (CIA) combined with "digital" confocal microscopy (DCM) was tested. DCM on serial image stacks of fluorescent latex beads reduced out-of-focus light propagation in the Z-axis (p < 0.005) and improved the shape factor of lateral three-dimensional reconstructions of the beads (p < 0.001). Sections of tissue infected for 44 hr with piliated, Opa+ gonococci were stained with fluorescein-labeled monoclonal anti-gonococcal antibodies, rhodamine-labeled phalloidin, and Hoechst 33342. Serial images collected at identical focal planes for each fluorochrome were subjected to DCM. Epithelial cytoplasmic regions of interest defined by rhodamine-stained actin were superimposed on the corresponding fluorescein-stained and Hoechst-stained images. Fluorescent objects defined by gray-scale threshold were measured by computerized image analysis using different border treatments to differentiate attached from intracellular gonococci or count cell nuclei. Compared with raw images, measurement of DCM images was less dependent on threshold choice (p < 0.05). DCM augments conventional microscopy in removing out-of-focus light from fluorescent images, in reconstructing three-dimensional images, and in quantitatively differentiating extracellular from intracellular gonococci in a natural target tissue.

Sign in / Sign up

Export Citation Format

Share Document