scholarly journals CD4 Masking during Human Immunodeficiency Virus Type 1 Infection, Quantified on Peripheral Blood Lymphocytes, Is a Potential Marker of Disease Progression

1996 ◽  
Vol 173 (3) ◽  
pp. 565-573 ◽  
Author(s):  
D. Carriere ◽  
J.-P. Vendrell ◽  
A.-M. Berthier ◽  
N. Atoui ◽  
S. L. Salhi ◽  
...  
Keyword(s):  
Human Immunodeficiency Virus ◽  
Disease Progression ◽  
Human Immunodeficiency Virus Type ◽  
Virus Type ◽  
Peripheral Blood ◽  
Peripheral Blood Lymphocytes ◽  
Blood Lymphocytes ◽  
Potential Marker ◽  
Immunodeficiency Virus

scholarly journals Human Immunodeficiency Virus Type 1 Modulates Telomerase Activity in Peripheral Blood Lymphocytes

2001 ◽  
Vol 183 (3) ◽  
pp. 417-424 ◽  
Author(s):  
Gianna Ballon ◽  
Lucia Ometto ◽  
Elisabetta Righetti ◽  
Anna Maria Cattelan ◽  
Sara Masiero ◽  
...  
Keyword(s):  
Human Immunodeficiency Virus ◽  
Human Immunodeficiency Virus Type ◽  
Virus Type ◽  
Peripheral Blood ◽  
Telomerase Activity ◽  
Peripheral Blood Lymphocytes ◽  
Blood Lymphocytes ◽  
Immunodeficiency Virus

scholarly journals Distinct Mechanisms Trigger Apoptosis in Human Immunodeficiency Virus Type 1-Infected and in Uninfected Bystander T Lymphocytes

1998 ◽  
Vol 72 (1) ◽  
pp. 660-670 ◽  
Author(s):  
Georges Herbein ◽  
Carine Van Lint ◽  
Jennie L. Lovett ◽  
Eric Verdin
Keyword(s):  
Human Immunodeficiency Virus ◽  
T Cells ◽  
T Lymphocytes ◽  
Human Immunodeficiency Virus Type ◽  
Virus Type ◽  
Peripheral Blood ◽  
Peripheral Blood Lymphocytes ◽  
Immunodeficiency Virus ◽  
Hiv 1

ABSTRACT Apoptosis is a main feature of AIDS pathogenesis and is thought to play a role in the progressive decrease of CD4+ T lymphocytes in infected individuals. To determine whether apoptosis occurs in infected and/or in uninfected peripheral blood T lymphocytes, we have used a recombinant human immunodeficiency virus type 1 (HIV-1) infectious clone expressing the green fluorescent protein (GFP). Using flow cytometry, we have determined the incidence of apoptosis by either terminal transferase dUTP nick end labeling or annexin-V assays in different cell subpopulations, i.e., in CD4+ or CD8+ T cells that were GFP positive or negative. After HIV-1 infection of purified peripheral blood lymphocytes, we observed that apoptosis occurred mostly in infected CD4+ peripheral blood lymphocytes. Remarkably, the presence of monocyte-derived macrophages in the culture increased dramatically the apoptosis of uninfected bystander T lymphocytes, while apoptosis in HIV-infected T lymphocytes was not changed. We therefore demonstrate that HIV-induced apoptosis results from at least two distinct mechanisms: (i) direct apoptosis in HIV-infected CD4+ T lymphocytes and (ii) indirect apoptosis in uninfected T cells mediated by antigen-presenting cells.

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