scholarly journals Emergence of vancomycin- and teicoplanin-resistant Enterococcus faecium via vanD5-harbouring large genomic island

2020 ◽  
Vol 75 (9) ◽  
pp. 2411-2415
Author(s):  
Toyotaka Sato ◽  
Takayuki Wada ◽  
Masaaki Shinagawa ◽  
Yukari Fukushima ◽  
Chie Nakajima ◽  
...  
Keyword(s):  
Gene Cluster ◽  
Genome Sequence ◽  
Enterococcus Faecium ◽  
Genetic Similarity ◽  
Genomic Island ◽  
Skin Ulcer ◽  
Whole Genome Sequence ◽  
Insertion Element ◽  
Vancomycin Resistant

Abstract Background Treatment of VRE is of clinical concern. While certain numbers of vanD-type VRE have been isolated, only two vanD5-harbouring Enterococcus faecium isolates have been reported in Canada and Japan. Methods We report the isolation of vanD5-type E. faecium and the first ever determination of the whole-genome sequence to investigate the possible mechanisms of the acquisition of the vanD5 gene cluster in E. faecium. Results Two vanD5-harbouring vancomycin-resistant E. faecium were isolated from the skin (SMVRE19) and faeces (SMVRE20) of a patient with a skin ulcer in Japan. The isolates exhibited vancomycin and teicoplanin MIC values of 128 mg/L, whilst the previous isolates of vanD5-harbouring E. faecium were only resistant to vancomycin. SMVRE19 and SMVRE20 were clones related to ST18, which is also seen in vanA- and vanB-type VRE. These isolates harboured an insertion element, ISEfm1, in the ddl gene, similar to a previously described teicoplanin-resistant vanD3-type E. faecium. The vanD5 gene cluster was integrated into the SMVRE20 chromosome as a part of a large genomic island (approximately 127 kb), similar to other recently spreading vanD variants in the Netherlands. The genomic island shared the greatest similarity with a part of the Blautia coccoides genome sequence, except for the region surrounding the vanD gene cluster. Conclusions This study reports that emergence of vancomycin- and teicoplanin-resistant vanD5-type E. faecium occurred via acquisition of the vanD5 cluster and ISEfm1 insertion into ddl. Considering the genetic similarity between the various VRE strains, the current study should serve as a warning against the spread of vanD5-type VRE.

scholarly journals Draft Genome Sequence of New Vancomycin-Resistant Enterococcus faecium Sequence Type 1421

2018 ◽  
Vol 6 (20) ◽  
Author(s):  
Kelvin W. C. Leong ◽  
Louise A. Cooley ◽  
Ronan F. O’Toole
Keyword(s):  
Genome Sequence ◽  
Enterococcus Faecium ◽  
Draft Genome ◽  
Sequence Type ◽  
Public Hospitals ◽  
Whole Genome Sequence ◽  
Vancomycin Resistant Enterococcus ◽  
Content Type ◽  
Vancomycin Resistant ◽  
Type Sequence

ABSTRACT The spread of vancomycin-resistant Enterococcus faecium (VREfm) has become a challenge to health care infection control worldwide. In 2015, a marked increase in VREfm isolation was detected in acute public hospitals in Tasmania. We report here the draft whole-genome sequence of a newly designated VREfm sequence type, sequence type 1421 (ST1421).

scholarly journals Whole Genome Sequence Analysis of the First Vancomycin-Resistant Enterococcus faecium Isolates from a Libyan Hospital in Tripoli

2020 ◽  
Vol 26 (11) ◽  
pp. 1390-1398
Author(s):  
Mohamed O. Ahmed ◽  
Asma K. Elramalli ◽  
Keith E. Baptiste ◽  
Mohamed A. Daw ◽  
Abdulaziz Zorgani ◽  
...  
Keyword(s):  
Sequence Analysis ◽  
Genome Sequence ◽  
Enterococcus Faecium ◽  
Whole Genome Sequence ◽  
Whole Genome ◽  
Vancomycin Resistant Enterococcus ◽  
Genome Sequence Analysis ◽  
Vancomycin Resistant

scholarly journals Novel Plasmid-Borne Multidrug Resistance Gene Cluster Includinglsa(E) from a Linezolid-Resistant Enterococcus faecium Isolate of Swine Origin

2015 ◽  
Vol 59 (11) ◽  
pp. 7113-7116 ◽  
Author(s):  
Hongbin Si ◽  
Wan-Jiang Zhang ◽  
Shengbo Chu ◽  
Xiu-Mei Wang ◽  
Lei Dai ◽  
...  
Keyword(s):  
Multidrug Resistance ◽  
Gene Cluster ◽  
Resistance Gene ◽  
Resistance Genes ◽  
Enterococcus Faecium ◽  
Insertion Element ◽  
Multidrug Resistance Gene ◽  
Content Type ◽  
Recombination Processes ◽  
Faecium Isolate

ABSTRACTA novel nonconjugative plasmid of 28,489 bp from a porcine linezolid-resistantEnterococcus faeciumisolate was completely sequenced. This plasmid harbored a novel type of multiresistance gene cluster that comprised the resistance geneslnu(B),lsa(E),spw,aadE,aphA3, and two copies oferm(B), which account for resistance to macrolides, lincosamides, streptogramins, pleuromutilins, streptomycin, spectinomycin, and kanamycin/neomycin. Structural comparisons suggested that this plasmid might have developed from other enterococcal plasmids by insertion element (IS)-mediated interplasmid recombination processes.

scholarly journals Draft Genome Sequence of a Vancomycin-Resistant and Vancomycin-Dependent Enterococcus faecium Isolate

2016 ◽  
Vol 4 (2) ◽  
Author(s):  
Marion Blaschitz ◽  
Sarah Lepuschitz ◽  
Laura Wagner ◽  
Franz Allerberger ◽  
Alexander Indra ◽  
...  
Keyword(s):  
Genome Sequence ◽  
Enterococcus Faecium ◽  
Prolonged Exposure ◽  
Draft Genome ◽  
Vancomycin Resistance ◽  
Draft Genome Sequence ◽  
Nosocomial Pathogens ◽  
Vancomycin Resistant Enterococci ◽  
Vancomycin Resistant ◽  
Faecium Isolate

Vancomycin-resistant enterococci have emerged as major nosocomial pathogens worldwide. While antimicrobial pressure promotes nosocomial colonization with these enterococci, prolonged exposure to vancomycin may foster the transition from vancomycin resistance to vancomycin dependence. Here, we report the draft genome sequence of a vancomycin-dependent Enterococcus faecium isolate showing partial teicoplanin dependence.

scholarly journals Identification of a Novel Genomic Island Associated withvanD-Type Vancomycin Resistance in Six Dutch Vancomycin-ResistantEnterococcus faeciumIsolates

2018 ◽  
Vol 62 (3) ◽  
Author(s):  
Janetta Top ◽  
Jan C. Sinnige ◽  
Ellen C. Brouwer ◽  
Guido Werner ◽  
Jukka Corander ◽  
...  
Keyword(s):  
Genetic Variation ◽  
Enterococcus Faecium ◽  
Genomic Island ◽  
Gene Clusters ◽  
Genomic Comparison ◽  
Variable Size ◽  
Content Type ◽  
Enterococcal Species ◽  
Vancomycin Resistant ◽  
Species Specific

ABSTRACTGenomic comparison of the first six DutchvanD-type vancomycin-resistantEnterococcus faecium(VRE) isolates with fourvanDgene clusters from other enterococcal species and anaerobic gut commensals revealed that thevanDgene cluster was located on a genomic island of variable size. Phylogenetic inferences revealed that the Dutch VRE isolates were genetically not closely related and that genetic variation of thevanD-containing genomic island was not species specific, suggesting that this island is transferred horizontally between enterococci and anaerobic gut commensals.

scholarly journals Determination of whole-genome sequence of Salmonella Abortusequi

2016 ◽  
Vol 39 ◽  
pp. S59 ◽  
Author(s):  
H. Niwa ◽  
M. Akiba ◽  
T. Sekizuka ◽  
M. Kuroda ◽  
Y. Kinoshita ◽  
...  
Keyword(s):  
Genome Sequence ◽  
Whole Genome Sequence ◽  
Whole Genome

scholarly journals Complete Genome Sequence and Annotation of the Paracoccus pantotrophus Type Strain DSM 2944

2020 ◽  
Vol 9 (1) ◽  
Author(s):  
Julia A. Bockwoldt ◽  
Martin Zimmermann ◽  
Till Tiso ◽  
Lars M. Blank
Keyword(s):  
Gene Cluster ◽  
Genome Sequence ◽  
Complete Genome Sequence ◽  
Complete Genome ◽  
Type Strain ◽  
Whole Genome Sequence ◽  
Whole Genome ◽  
Content Type ◽  
Paracoccus Pantotrophus

Paracoccus spp. are metabolically versatile alphaproteobacteria able to perform heterotrophic and chemoautotrophic growth. This study describes the whole-genome sequence of the Paracoccus pantotrophus type strain DSM 2944 (ATCC 35512, LMD 82.5, GB17). The genome sequence revealed the presence of a complete phaZ phaC phaP phaR gene cluster related to polyhydroxyalkanoate metabolism.

scholarly journals Complete Genome Sequence of a Vancomycin-Resistant Sequence Type 203 Enterococcus faecium Strain with vanA Belonging to Complex Type 859

2018 ◽  
Vol 7 (2) ◽  
Author(s):  
Maria Hoffmann ◽  
Kuan Yao ◽  
Marc Allard ◽  
Maria Sanchez ◽  
Leif P. Andersen ◽  
...  
Keyword(s):  
Genome Sequence ◽  
Complete Genome Sequence ◽  
Enterococcus Faecium ◽  
Complete Genome ◽  
Sequence Type ◽  
Complex Type ◽  
Content Type ◽  
Vancomycin Resistant ◽  
Faecium Strain

In 2014, the first vancomycin-resistant (encoded by vanA) Enterococcus faecium isolate belonging to sequence type 203 (ST203) and complex type 859 (CT859) was detected in Denmark. In 2016, 64% of the Danish clinical vanA E. faecium isolates belonged to ST203 and CT859.

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