Colorimetric Analysis of Norethindrone, Norethindrone Acetate, and Norgestrel in Oral Contraceptives

1970 ◽  
Vol 53 (5) ◽  
pp. 1089-1092
Author(s):  
Robert V Smith ◽  
Thomas H Hassall ◽  
Shuing Carol Liu
Keyword(s):  
Oral Contraceptives ◽  
Ethinyl Estradiol ◽  
Colorimetric Method ◽  
Colorimetric Analysis ◽  
Blue Tetrazolium ◽  
Norethindrone Acetate

Abstract A colorimetric method, based on reaction with blue tetrazolium, has been devised for the analysis of norethindrone, norethindrone acetate, and norgestrel. The technique is selective for these progestins, showing no interference from the estrogens ethinyl estradiol and mestranol, commonly found in oral contraceptives. The developed procedures have been successfully applied to the determination of progestins in anovulatory tablets.

Collaborative Study of a Semiautomated Method for the Analysis of Prednisolone and Prednisone Tablets

1977 ◽  
Vol 60 (1) ◽  
pp. 27-31
Author(s):  
James F Brower
Keyword(s):  
Collaborative Study ◽  
Colorimetric Method ◽  
Alcoholic Solution ◽  
Tetramethylammonium Hydroxide ◽  
Assay Method ◽  
Blue Tetrazolium ◽  
Coefficients Of Variation ◽  
The Individual ◽  
Semiautomated Method

Abstract A semiautomated colorimetric method for the determination of prednisolone and prednisone was collaboratively studied by 6 collaborators. In the method, an alcoholic solution of the drug is extracted with chloroform and the extract is reacted with tetramethylammonium hydroxide and blue tetrazolium; the absorbance of the resulting color is read at 525 nm. Collaborators were supplied with 4 composites of tablets of different dosage levels, 2 containing prednisolone and 2 containing prednisone. Results agreed with those obtained by the author using the CSP total steroid assay method. The coefficients of variation of the individual collaborator’s results for prednisolone and prednisone ranged from 0.54 to 2.38 and from 0.34 to 2.19%, respectively. This method has been adopted as official first action.

Simultaneous Determination of Phosphorus, Iron, and Aluminum in Phosphate Rock and Fertilizer Systems by Automated Colorimetric Analysis

1969 ◽  
Vol 52 (6) ◽  
pp. 1121-1126
Author(s):  
Jack L Hoyt ◽  
Donald E Jordan
Keyword(s):  
Phosphate Rock ◽  
Colorimetric Method ◽  
Iron Complex ◽  
Colorimetric Analysis ◽  
Splitting Technique ◽  
Phosphorus Iron ◽  
Effective Analysis ◽  
Better Than

Abstract An automated colorimetric method is used to simultaneously determine phosphorus, iron, and aluminum in phosphate rock and various solid and liquid fertilizer systems. Combination of an in situ dilution technique for phosphorus with a sample splitting technique results in an effective analysis rate of 60, 90, or 120 determinations/hr (20, 30, or 40 samples/hr, 3 elements/sample). Phosphorus is determined as the molybdovanadophosphate complex at 420 mμ; iron and aluminum are both determinated as “Ferron” (8-hydroxy-7-iodo-5-quinoline sulfonic acid) complexes with the iron complex determined at 610 mμ and combined iron and aluminum complexes determined at 366 mμ. The precision and accuracy are better than ±1% relative throughout the total range of 0.1 to 75% P2O5, and up to 10% Fe2O3 and 10% AL2O3.

scholarly journals Unilateral Erythema Nodosum following Norethindrone Acetate, Ethinyl Estradiol, and Ferrous Fumarate Combination Therapy

2016 ◽  
Vol 2016 ◽  
pp. 1-3
Author(s):  
Michelle S. Min ◽  
Rob Fischer ◽  
John B. Fournier
Keyword(s):  
Oral Contraceptive ◽  
Combination Therapy ◽  
Oral Contraceptives ◽  
Erythema Nodosum ◽  
Ethinyl Estradiol ◽  
Oral Contraceptive Pills ◽  
Ferrous Fumarate ◽  
Contraceptive Pills ◽  
Secondary Causes ◽  
Norethindrone Acetate

Erythema nodosum is a septal panniculitis that typically presents as symmetric, tender nodules on the anterior aspects of bilateral lower extremities. Nearly half of cases are due to secondary causes, with oral contraceptive pills being the leading pharmaceutical cause. However, to our knowledge, there has yet to be a published association with norethindrone acetate, ethinyl estradiol, and ferrous fumarate. We report our experience with a 30-year-old woman who developed unilateral tender nodules within a month of starting 1 mg norethindrone acetate and 20 mcg ethinyl estradiol daily. Of note, she had previously taken oral contraceptives with the same estrogen agent but different progesterone, without problems. We conclude that systemically triggered erythema nodosum can present with lesions localized to one extremity. When a patient presents with tender, firm nodules, clinicians should consider the possibility of erythema nodosum and its triggers, such as oral contraceptives. Additionally, should a patient on hormonal therapy develop erythema nodosum, changing the progesterone agent may allow the patient to continue similar therapy without developing symptoms.

Rapid, Sensitive Colorimetric Method for Determination of Ethinyl Estradiol

1975 ◽  
Vol 64 (7) ◽  
pp. 1221-1223 ◽  
Author(s):  
Mohamed A. Eldawy ◽  
A.S. Tawfik ◽  
S.R. Elshabouri
Keyword(s):  
Ethinyl Estradiol ◽  
Colorimetric Method

Use of a low-dose oral contraceptive containing norethindrone acetate and ethinyl estradiol in the treatment of moderate acne vulgaris

2001 ◽  
Vol 1 (3) ◽  
pp. 123-131 ◽  
Author(s):  
J. Michael Maloney ◽  
Deborah I. Arbit ◽  
Mary Flack ◽  
Constance McLaughlin-Miley ◽  
Cynthia Sevilla ◽  
...  
Keyword(s):  
Oral Contraceptive ◽  
Low Dose ◽  
Ethinyl Estradiol ◽  
Acne Vulgaris ◽  
Dose Oral ◽  
Dose Oral Contraceptive ◽  
Norethindrone Acetate

Decreased Levels of Histidine-Rich Glycoprotein (HRG) and Increased Levels of Free Plasminogen in Women on Oral Contraceptives Low in Estrogen

1982 ◽  
Vol 48 (03) ◽  
pp. 283-285 ◽  
Author(s):  
Jørgen Jespersen ◽  
Cornelis Kluft
Keyword(s):  
Oral Contraceptives ◽  
Ethinyl Estradiol ◽  
Relative Increase ◽  
Normal Group ◽  
Plasma Samples ◽  
Normal Women ◽  
Made In

SummaryDeterminations by immunologic methods of histidine-rich glycoprotein (HRG) and plasminogen, were made in plasma samples collected during one normal or hormone induced cycle in 15 young, normal women and in 11 women using oral contraceptives with 30 μg ethinyl estradiol and 150 μg levo-norgestrel. The hormone group showed an increase in plasminogen level to about 150% of normal, while the concentration of HRG was decreased to about 75% of normal. This resulted in a considerable relative increase in the concentration of free plasminogen in the hormone group (calculated from the equilibrium: HRG·plasminogen ⇌ HRG + plasminogen, using KD = 1.0 μM), representing a doubling of that in the normal group. Hence, more plasminogen is available for binding to fibrin and activation in the hormone group.

The Need for Using An Enzymatic Colorimetric Assay in Creatinine Determination of Peritoneal Dialysis Solutions

1990 ◽  
Vol 10 (1) ◽  
pp. 89-92 ◽  
Author(s):  
Liliane Larpent ◽  
Christian Verger
Keyword(s):  
Peritoneal Dialysis ◽  
Reliable Method ◽  
Colorimetric Assay ◽  
Colorimetric Method ◽  
Peritoneal Membrane ◽  
Creatinine Kinetics ◽  
Dialysis Solutions ◽  
Peritoneal Dialysis Solutions ◽  
Enzymatic Test

The fate of the peritoneal membrane on continuous ambulatory peritoneal dialysis (CAPD) is usually evaluated through the modification of its permeability to various solutes as glucose, creatinine, and urea. Therefore, the accuracy of the methods used for measurements of creatinine is of great importance. A particular problem does exist for creatinine determination as it may be influenced by the presence of glucose. We studied a new enzymatic colorimetric method for creatinine determination in peritoneal dialysis solutions which contain high dextrose concentrations. Creatinine was measured in plasma, urine, and dialysate from 18 patients on CAPD and in pure dextrose solutions, with an enzymatic test (Boehringer Mannheim) and with Jaffe's reaction on two different analyzers: Astra (Beckman) and Eris (Merck). Creatinine results were similar with both assays (Jaffe's reaction and enzymatic test) when measured in blood and urine. However the Jaffe's reaction gave higher creatinine results than the enzymatic test (p < 0.001), when assays were performed in peritoneal dialysis solutions and in pure glucose solutions. In addition, it appeared that other components of dialysis solutions, mainly calcium chloride, influenced unpredictably the results of creatinine with the Jaffe's reaction. We conclude that specific enzymatic test is a more accurate and reliable method to evaluate creatinine kinetics through the peritoneal membrane when determined in CAPD solutions.

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