Quantitative Thin Layer Chromatographic Determination of Furazolidone and Nitrofurazone in Animal Feeds

1978 ◽  
Vol 61 (1) ◽  
pp. 92-95
Author(s):  
Ugo R Cieri
Keyword(s):  
Thin Layer ◽  
Ultraviolet Light ◽  
Internal Standard ◽  
Chromatographic Determination ◽  
Animal Feeds ◽  
Recording Spectrophotometer ◽  
Tungsten Lamp

Abstract A method is presented for determining the nitrofurans furazolidone and nitrofurazone in animal feeds. The sample is extracted with acetone, and aliquots of the concentrated extract are spotted on thin layer chromatographic fluorescent plates. After development in CHCl3-methanol (90+10), the bands containing the nitrofurans are detected under shortwave ultraviolet light, scraped from the plate, and extracted with ethanol. The centrifuged extracts are scanned from 500 to 300 nm on a recording spectrophotometer with a tungsten lamp, and the absorbance maxima near 360 nm are used for quantitation. To compensate for extraction and chromatographic losses and for other interferences, the nitrofuran not present in the sample is added as an internal standard. The method is generally not applicable at a level below 0.005% since detection of the bands becomes difficult.

Thin Layer Chromatographic Determination of Ctrinin

1979 ◽  
Vol 62 (3) ◽  
pp. 570-572
Author(s):  
Ramaswamy V Chalam ◽  
Henry M Stahr
Keyword(s):  
Aqueous Solution ◽  
Thin Layer ◽  
Ultraviolet Light ◽  
Chromatographic Determination ◽  
Chloroform Extract ◽  
Corn Silage ◽  
Aqueous Layer ◽  
Mixed Feeds ◽  
Feed Samples

Abstract A thin layer chromatographic (TLC) method is described for the determination of citrinin in feeds. Citrinin is extracted from feed with methanol and water, the mixture is made alkaline with 10% sodium carbonate, and the aqueous solution is filtered and extracted with chloroform to remove most of the interfering materials. The aqueous layer is acidified with 2N HCl and extracted with chloroform. The chloroform extract is concentrated and spotted on a thin layer chromatographic (TLC) plate which is developed in chloroform-acetoneethanol- water (60+40+10+1). The citrinin is viewed under ultraviolet light after TLC. Either visual or fiuorodensitometric quantitation is used. Recoveries of citrinin from various feed samples spiked at levels of 2.0–5 μg/g were 75–92%. The proposed method can detect 0.5 μg/g feed, including corn, silage, ready mixed feeds, and feed pellets.

Thin Layer Chromatographic Determination of Deoxynivalenol in Wheat and Corn

1984 ◽  
Vol 67 (1) ◽  
pp. 40-43 ◽  
Author(s):  
Mary W Trucksess ◽  
Stanley Nesheim ◽  
Robert M Eppley
Keyword(s):  
Thin Layer ◽  
Ethyl Acetate ◽  
Ultraviolet Light ◽  
Silica Gel ◽  
Steam Bath ◽  
Chromatographic Determination ◽  
Detectable Amount ◽  
Fluorescent Spot ◽  
Minimum Detectable Amount

Abstract A thin layer chromatographic (TLC) method for determining deoxynivalenol (DON) in corn and wheat was developed. DON is extracted from the grain with acetonitrile–water (84 + 16) and filtered through a column of mixed alumina–charcoal–Celite (0.5 g + 0.7 g + 0.3 g). The solvent is evaporated on a steam bath. Ethyl acetate is added to the residue and heated to dissolve DON. After cooling, the residue is transferred to a vial with additional ethyl acetate and is dissolved in CHCl3–acetonitrile (4 + 1) for TLC on an AlCl3-impregnated silica gel plate with CHCI3–acetone–isopropanol (8 + 1 + 1). The plate is heated in a 120°C oven for 7 min; a blue fluorescent spot is produced under longwave ultraviolet light. DON is quantitated visually and/or fluorodensitometrically by comparison with reference standards. The minimum detectable amount of DON is ca 20 ng/spot. The limit of DON determination is ca 40 ng/g for wheat and 100 ng/g for corn. Recoveries of DON added to wheat and corn at 100, 500, and 1000 ng/ g levels were 85, 93, and 88% and 77, 80, and 80%, respectively.

High-performance thin-layer chromatographic determination of rosiglitazone in its dosage form

2002 ◽  
Vol 15 (3) ◽  
pp. 192-195 ◽  
Author(s):  
Ramesh Sane ◽  
Mary Francis ◽  
Atul Moghe ◽  
Sachin Khedkar ◽  
Ajit Anerao
Keyword(s):  
Thin Layer ◽  
High Performance ◽  
Dosage Form ◽  
Chromatographic Determination

Improved thin-layer chromatographic determination of phospholipids in gastric aspirate from newborns, for assessment of lung maturity.

1988 ◽  
Vol 34 (4) ◽  
pp. 736-738 ◽  
Author(s):  
D Serrano de la Cruz ◽  
E Santillana ◽  
A Mingo ◽  
G Fuenmayor ◽  
A Pantoja ◽  
...  
Keyword(s):  
Thin Layer ◽  
Fetal Lung ◽  
Chromatographic Determination ◽  
Solvent Mixture ◽  
Gastric Aspirate ◽  
One Dimensional ◽  
Acetic Acid Water ◽  
Chromatographic Resolution ◽  
Lung Maturity

Abstract This one-dimensional thin-layer chromatographic method is used for assay of phospholipids in the gastric aspirate of newborns. The solvent mixture (chloroform/hexane/methanol/glacial acetic acid/water, 12/7/4/3/0.3 by vol) completely resolves lecithin, sphingomyelin, phosphatidylinositol, phosphatidylserine, phosphatidylethanolamine, and phosphatidylglycerol. The method is simple, precise, inexpensive, and rapid (chromatographic development takes less than 25 min) and gives high chromatographic resolution. We used this method to determine the lecithin/sphingomyelin densitometric ratio (L/S ratio) and the phosphatidylglycerol percentage in 200 samples of gastric aspirate and found an L/S ratio of 2.5 to be a satisfactory cutoff value for distinguishing fetal lung maturity and immaturity. We confirmed that the presence of phosphatidylglycerol excluded the possibility of respiratory distress.

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