General Method for Determination of Pesticide Residues in Samples of Plant Origin, Soil, and Water. II. Thin Layer Chromatographic Determination

1981 ◽  
Vol 64 (3) ◽  
pp. 743-748 ◽  
Author(s):  
Árpád Ambrus ◽  
Éva Hargitai ◽  
Gabriella Károly ◽  
András Fülöp Ambrus ◽  
János Lantos Ambrus
Keyword(s):  
Thin Layer ◽  
Pesticide Residues ◽  
Unknown Origin ◽  
Chromatographic Determination ◽  
Plant Origin ◽  
Coefficients Of Variation ◽  
Rf Values ◽  
Soil And Water ◽  
General Method

Abstract o-Tolidine plus KI, p-nitrobenzene-diazoniumfluoroborate, bioassay with fungi and enzyme sources, AgNO3 plus UV radiation, and p-dimethyl-aminobenzaldehyde modes of detection have been selected for TLC screening of pesticide residues in extracts of samples of unknown origin. Single solvents were used for the elution. Coefficients of variation of Rf values were studied as a function of Rf and eluants. Indicator compounds were used for controlling the proper conditions of detection. The detectability of 188 pesticide compounds was tested, and the minimum detectable amounts were determined.

Additional Cleanup of Samples on GLC Column for TLC Determination of Pesticide Residues

1967 ◽  
Vol 50 (3) ◽  
pp. 615-623
Author(s):  
Kenneth T Hartman
Keyword(s):  
Liquid Chromatography ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Pesticide Residues ◽  
Gas Liquid Chromatography ◽  
Rf Values ◽  
Layer Chromatography

Abstract Gas-liquid chromatography (GLC) following conventional isolation procedures has been used to clean up pesticide residues for confirmation by thin layer chromatography (TLC). This procedure is more rapid and efficient than present cleanup procedures and permits the determination of pesticide residues that do not survive these rigorous acid or alkali treatments. The method also permits TLC confirmation of pesticide residues that have similar Rf values but different GLC retention times. Recoveries ranged from 85 to 105% for 25 of 28 pesticides tested

Thin Layer Chromatographic Determination of Aflatoxins, Ochratoxins, Sterigmatocystin, Zearalenone, Citrinin, T-2 Toxin, Diacetoxyscirpenol, Penicillic Acid, Patulin, and Penitrem A

1979 ◽  
Vol 62 (3) ◽  
pp. 579-585 ◽  
Author(s):  
Alberto Gimeno
Keyword(s):  
Thin Layer ◽  
Chromatographic Determination ◽  
Minimum Detectable Concentration ◽  
Penicillic Acid ◽  
Detectable Concentration ◽  
Mixed Feeds ◽  
Limit Detection ◽  
Layer Chromatography ◽  
General Method

Abstract A general method is described for determining 16 mycotoxins in mixed feeds and other food products used in the manufacture of these feedstuffs. The mycotoxins are extracted and cleaned up by extracting with solvents of different pH. Thin layer chromatography is used to separate the toxins; toxins are then quantitated by the limit detection method. The minimum detectable concentration of mycotoxins in various products is: aflatoxin B1 or G1, 4–5 μg/kg; cchratoxin A or ethyl ester A 140–145 μg/kg; citrinin 600–750 μg/kg; zearalenone, 410–500 μg/kg; sterigmatocystin, 140–145 μg/kg; diacetoxyscirpenol, 2400–2600 μg/kg; T-2 toxin, 800-950 μg/kg; patulin, 750-800 μg/kg; penitrem A 14,000–14,500 μg/kg; penicillic acid 3400-3650 μg/kg.

The thin-layer chromatographic determination of triazine herbicides in soil and water

The Analyst ◽  
1965 ◽  
Vol 90 (1071) ◽  
pp. 356 ◽  
Author(s):  
D. C. Abbott ◽  
J. A. Bunting ◽  
J. Thomson
Keyword(s):  
Thin Layer ◽  
Chromatographic Determination ◽  
Triazine Herbicides ◽  
Soil And Water

General Method for Determination of Pesticide Residues in Samples of Plant Origin, Soil, and Water. III. Gas Chromatographic Analysis and Confirmation

1981 ◽  
Vol 64 (3) ◽  
pp. 749-768
Author(s):  
Árpád Ambrus ◽  
Éva Visi ◽  
Ferenc Zakar ◽  
Éva Hargitai ◽  
László Szabó ◽  
...  
Keyword(s):  
Chromatographic Analysis ◽  
Residue Analysis ◽  
Gas Liquid Chromatography ◽  
Pesticide Residue Analysis ◽  
Temperature Programming ◽  
Plant Origin ◽  
Relative Retention ◽  
Soil And Water ◽  
General Method

Abstract Gas-liquid chromatography is widely used in pesticide residue analysis, and numerous packings of various polarity are recommended. We selected OV-22, OV-101, NPGS, SE-30 (3% on Gas-Chrom Q), and 1.95% SP-2401/1.5% SP-2250 on Supelcoport as basic packings. Relative retention times for aldrin and parathion-methyl were determined at 140,160, 180, or 200°C and during temperature programming. OV-22 and OV-101 were most suitable for general purposes. A study on the performance of thermionic delectors suggested that their performance can be characterized and should be regularly controlled with test substances containing different hetero elements. Columns as short as 45-90 cm were suitable for screening purposes, and their advantages over traditional long columns are demonstrated. The fractionation of pesticides in an extract on silica gel columns was effective and practical for the confirmation of pesticides.

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