Thin Layer Chromatographic Determination of Methyl Parathion as Paraoxon by Cholinesterase Inhibition

1981 ◽  
Vol 64 (6) ◽  
pp. 1312-1314
Author(s):  
Sana Udaya Bhaskar ◽  
Nanguneri V Nanda Kumar
Keyword(s):  
Thin Layer ◽  
Rapid Method ◽  
Methyl Parathion ◽  
Quantitative Estimation ◽  
Chromatographic Determination ◽  
Acetone Powder ◽  
Cholinesterase Inhibition ◽  
Pig Liver ◽  
Nitrophenyl Phosphate

Abstract A simple, sensitive, and rapid method is described for the quantitative estimation of nanogram amounts of methyl parathion (O,O-dimethyl O-p-nitrophenyl phosphorothioate) as methyl paraoxon (O,O-dimethyl O-p-nitrophenyl phosphate) on thin layer chromatograms. Methyl paraoxon is detected by pig liver acetone powder cholinesterase inhibition, using p-nitrobenzenediazoniumfluoroborate as the chromogenic reagent. Commercial pig liver acetone powder is more advantageous than raw liver sources because it is readily available and can be preserved indefinitely. About 0.1 ng methyl parathion can be detected, and amounts from 5 to 50 ng can be quantitatively estimated.

Thin Layer Chromatography of Parathion as Paraoxon with Cholinesterase Inhibition Detection

1976 ◽  
Vol 59 (3) ◽  
pp. 641-643
Author(s):  
Nanguneri V Nanda Kumar ◽  
Krishnamurthy Visweswariah ◽  
Suvendu K Majumder
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Rapid Method ◽  
Chromatographic Analysis ◽  
Quantitative Estimation ◽  
Viable Alternative ◽  
Cholinesterase Inhibition ◽  
Chromogenic Reagent ◽  
Nitrophenyl Phosphate ◽  
Layer Chromatography

Abstract A simple, sensitive, and rapid method is described for the quantitative estimation of ng amounts of parathion (O,O-diethyl O-p-nitrophenyl phosphorothioate) as paraoxon (O,O-diethyl O-p-nitrophenyl phosphate) on thin layer chromatograms. Paraoxon is detected by inhibition, using p-nitrobenzenediazonium fluoroborate as the chromogenic reagent. This chromogenic reagent is more sensitive than Fast Blue B or indoxyl acetate; 0.1 ng may be detected and amounts from 5 to 50 ng may be estimated. The method is a viable alternative to gas chromatographic analysis for parathion.

Colorimetric Determination of Methyl Parathion and Oxygen Analog

1980 ◽  
Vol 63 (3) ◽  
pp. 536-538
Author(s):  
Nanguneri V Nanda Kumar ◽  
Mattipalli Ramasundari
Keyword(s):  
Methyl Parathion ◽  
Colorimetric Method ◽  
Acetone Powder ◽  
Colorimetric Determination ◽  
Pig Liver ◽  
Oxygen Analog ◽  
Nitrophenyl Phosphate ◽  
Colorimetric Methods ◽  
Nonenzymatic Reactions

Abstract A simple, sensitive, and rapid colorimetric method is described (or determining methyl parathion (0,0-dimethyl-O-ρ-nitrophenyl phosphorothioate) and methyl paraoxon (O,O-dimethyl-O-ρ-nitrophenyl phosphate), using ρ-nitrobenzene diazonium fluoroborate as the chromogenic salt. This colorimetric method is more sensitive than are other colorimetric methods based on nonenzymatic reactions. Pig liver acetone powder cholinesterase was found to be sensitive to methyl parathion. Inhibition can be detected at picogram levels, and 50–80 ng methyl paraoxon and 1–9 μg methyl parathion can be estimated.

Thin Layer Chromatographic Determination of Sodium Monofluoroacetate

1970 ◽  
Vol 53 (4) ◽  
pp. 737-741
Author(s):  
Vernon A Perry
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Ammonium Salt ◽  
Quantitative Estimation ◽  
Chromatographic Determination ◽  
Animal Tissue ◽  
Layer Chromatography

Abstract A microanalytical method for the detection and quantitative estimation of the rodenticide sodium monofluoracetate from animal tissue has been developed. This procedure uses thin layer chromatography, requires little or no special cleanup, and will detect as little as 0.1 Mg monofluoroacetate as the ammonium salt.

High-performance thin-layer chromatographic determination of rosiglitazone in its dosage form

2002 ◽  
Vol 15 (3) ◽  
pp. 192-195 ◽  
Author(s):  
Ramesh Sane ◽  
Mary Francis ◽  
Atul Moghe ◽  
Sachin Khedkar ◽  
Ajit Anerao
Keyword(s):  
Thin Layer ◽  
High Performance ◽  
Dosage Form ◽  
Chromatographic Determination

Determination of Dimetridazole and Ipronidazole in Feeds at Cross-Contamination Levels

1988 ◽  
Vol 71 (3) ◽  
pp. 474-477 ◽  
Author(s):  
Duane D Hughes
Keyword(s):  
Thin Layer ◽  
Rapid Method ◽  
Aqueous Phase ◽  
Borate Buffer ◽  
Cross Contamination ◽  
Chromatographic System ◽  
Arsanilic Acid ◽  
Contamination Levels ◽  
Benzene Extract

Abstract A rapid method for the determination of dimetridazole and ipronidazole in feeds is described. The compounds are extracted from a borate buffer (pH 8.65) with benzene, partitioned into IN HC1, and then partitioned back into benzene from a basic aqueous phase. The benzene extract is concentrated and injected onto a nonpolar (Apiezon L) gas chromatographic column for determination by 63Ni electroncapture detection. Recoveries from feeds of various composition, spiked at 0.2 ppm with both dimetridazole and ipronidazole, ranged from 70 to 115%; for the same feeds spiked at 1 ppm or more, the recoveries were greater than 80%. Carbadox, furazolidone, levamisole, oxytetracycline, chlortetracycline, sulfamethazine, sulfaquinoxaline, arsanilic acid, piperazine, penicillin, and commonly added vitamins and minerals do not interfere. A 2-dimensional thin layer chromatographic system is presented as a means of additional identification.

Improved thin-layer chromatographic determination of phospholipids in gastric aspirate from newborns, for assessment of lung maturity.

1988 ◽  
Vol 34 (4) ◽  
pp. 736-738 ◽  
Author(s):  
D Serrano de la Cruz ◽  
E Santillana ◽  
A Mingo ◽  
G Fuenmayor ◽  
A Pantoja ◽  
...  
Keyword(s):  
Thin Layer ◽  
Fetal Lung ◽  
Chromatographic Determination ◽  
Solvent Mixture ◽  
Gastric Aspirate ◽  
One Dimensional ◽  
Acetic Acid Water ◽  
Chromatographic Resolution ◽  
Lung Maturity

Abstract This one-dimensional thin-layer chromatographic method is used for assay of phospholipids in the gastric aspirate of newborns. The solvent mixture (chloroform/hexane/methanol/glacial acetic acid/water, 12/7/4/3/0.3 by vol) completely resolves lecithin, sphingomyelin, phosphatidylinositol, phosphatidylserine, phosphatidylethanolamine, and phosphatidylglycerol. The method is simple, precise, inexpensive, and rapid (chromatographic development takes less than 25 min) and gives high chromatographic resolution. We used this method to determine the lecithin/sphingomyelin densitometric ratio (L/S ratio) and the phosphatidylglycerol percentage in 200 samples of gastric aspirate and found an L/S ratio of 2.5 to be a satisfactory cutoff value for distinguishing fetal lung maturity and immaturity. We confirmed that the presence of phosphatidylglycerol excluded the possibility of respiratory distress.

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