Rapid Detection of Fusarium Mycotoxins in Grains by Quadrupole Mass Spectrometry/Mass Spectrometry

1983 ◽  
Vol 66 (6) ◽  
pp. 1470-1477
Author(s):  
Ronald D Plattner ◽  
Glenn A Bennett
Keyword(s):  
Mass Spectrometry ◽  
Rapid Detection ◽  
Negative Ion ◽  
Spectrometric Analysis ◽  
Fusarium Mycotoxins ◽  
Quadrupole Mass ◽  
Quadrupole Mass Spectrometry ◽  
Collisionally Activated Dissociation ◽  
Authentic Standard ◽  
Mass Spectrometry Mass Spectrometry

Abstract The Fusarium mycotoxins deoxynivalenol (DON) and zearalenone can be detected rapidly in a crude extract of a grain matrix by quadrupole mass spectrometry/ mass spectrometric analysis of the collisionally activated dissociation (CAD) daughter fragments of the protonated molecule. Detection limits were as low as 0.1 ppm. The 2 toxins were detected in a single analysis. Monitoring the CATJ daughters of the negative ion m/z 248 of DON eliminated most interferences from the grain matrix and produced daughter spectra identical to those produced from an authentic standard.

Identification of Aflatoxins by Quadrupole Mass Spectrometry/Mass Spectrometry

1984 ◽  
Vol 67 (4) ◽  
pp. 734-738 ◽  
Author(s):  
Ronald D Plattner ◽  
Glenn A Bennett ◽  
Robert D Stubblefield
Keyword(s):  
Mass Spectrometry ◽  
Aflatoxin B1 ◽  
Impact Ionization ◽  
Negative Ion ◽  
Quadrupole Mass ◽  
Molecular Anion ◽  
Negative Ion Mode ◽  
Protonated Molecules ◽  
Positive Ion ◽  
Mass Spectrometry Mass Spectrometry

Abstract MS/MS daughter experiments were recorded for aflatoxins B1, B2, G1, G2, M1, M2, and aflatoxicol, using 3 ionization modes. Daughters were recorded from the molecular ion (M+) using electron impact ionization (EI). Daughters from the protonated molecules (MH+) were recorded in the positive ion mode and the daughters from the molecular anion (M+) were recorded in the negative ion mode using chemical ionization (CI). These daughter spectra are all relatively simple. The EI daughters are quite similar to conventional EI spectra. The yield of (M-) is about 100 times greater than the yield of M+ in EI or MH+ in isobutane CI spectrum. Negative ion daughter spectra were used to demonstrate the feasibility of determining the presence of aflatoxin B1 in crude extracts of contaminated corn. Aflatoxin B1 could be detected at 10 ppb.

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