Liquid Chromatographic Determination of Hypoxanthine Content in Fish Tissue

1985 ◽  
Vol 68 (3) ◽  
pp. 444-448 ◽  
Author(s):  
Garth B Burns ◽  
Paul J Ke
Keyword(s):  
Potassium Phosphate ◽  
Adenosine Monophosphate ◽  
Fish Tissue ◽  
Chromatographic Determination ◽  
Fish Tissues ◽  
Absorbance Detection ◽  
Relative Standard ◽  
Operational Errors ◽  
Liquid Chromatographic

Abstract A liquid chromatography (LC) method for determining the hypoxanthine content in fish tissues has been developed. Hypoxanthine is extracted with 0.6M perchloric acid, and determined by LC on a reverse phase microparticulate column with UV absorbance detection. The mobile phase is 0.01M potassium phosphate buffer (pH 4.5). The percent relative standard deviation for measurements by the recommended method was less than 7% with a detection limit of 10 ng. Recoveries of hypoxanthine added to various fish tissues were better than 90%. The operational errors, interferences, and recoveries for spiked samples have been investigated and compare favorably with an established xanthine oxidase enzyme method. The described LC method is simple, rapid, and specific for measuring hypoxanthine content in various fish tissues. Some post-mortem studies have indicated the method may also be used for the determination of adenosine monophosphate, inosine monophosphate, and inosine.

Liquid Chromatographic Determination of Glyphosate and Aminomethylphosphonic Acid (AMPA) in Environmental Water: Collaborative Study

1991 ◽  
Vol 74 (2) ◽  
pp. 317-323 ◽  
Author(s):  
Mark E Oppenhuizen ◽  
John E Cowell
Keyword(s):  
Collaborative Study ◽  
Chromatographic Determination ◽  
Environmental Water ◽  
Aminomethylphosphonic Acid ◽  
Relative Standard ◽  
Total Variability ◽  
Liquid Chromatographic Determination ◽  
Predicted Values ◽  
Liquid Chromatographic

Abstract A new method for determination of glyphosate and amlnomethylphosphonlc acid (AMPA) residues In environmental water was collaboratively studied by 6 laboratories. The method Is simpler and shorter than previous methods. A filtered volume of water is evaporated to dryness and the residue Is dissolved In a buffered EDTA solution. Glyphosate and AMPA are determined by liquid chromatography with postcolumn reaction detection. The method was validated over the range 0.50-5000 ppb, although one of the collaborating laboratories could not reliably quantltate below 1.0 ppb. Statistical analysis of the results showed that typical reproducibility relative standard deviations (RSDR) ranged from 11 to 20% for both glyphosate and AMPA, which compares very well with predicted values for this concentration range. Total variability (as measured by sR) Increased with increasing fortification level. The method has been adopted official first action by AOAC.

scholarly journals Liquid Chromatographic Determination of Deoxynivalenol in Baby Food and Animal Feed: Interlaboratory Study

2006 ◽  
Vol 89 (4) ◽  
pp. 1012-1020 ◽  
Author(s):  
Joerg Stroka ◽  
Michelle Derbyshire ◽  
Carsten Mischke ◽  
Massimo Ambrosio ◽  
Katy Kroeger ◽  
...  
Keyword(s):  
Standard Deviation ◽  
Relative Standard Deviation ◽  
High Performance ◽  
Animal Feed ◽  
Chromatographic Determination ◽  
Baby Food ◽  
Interlaboratory Study ◽  
Relative Standard ◽  
Liquid Chromatographic

Abstract An interlaboratory study was conducted for the determination of deoxynivalenol in baby food and animal feed by high-performance liquid chromatography with UV detection. The study included 14 participants representing a cross section of industry, official food control, and research facilities. Mean recoveries reported ranged from 89% (at 120 g/kg) to 85% (at 240 g/kg) for baby food and from 100% (at 200 g/kg) to 93% (at 400 g/kg) for animal feed. On the basis of the results for spiked samples (blind duplicates at 2 levels), as well as those for naturally contaminated samples (blind duplicates at 3 levels), the relative standard deviation for repeatability (RSDr) in analyses of baby food ranged from 6.4 to 14.0% and in analyses of animal feed, from 6.1 to 16.5%. The relative standard deviation for reproducibility (RSDR) in analyses of baby food ranged from 9.4 to 19.5% and in analyses of animal feed, from 10.5 to 25.2%. The HorRat values ranged from 0.4 to 1.0 and from 0.7 to 1.3, for baby food and animal feed, respectively. The method showed acceptable performance for within-laboratory and between-laboratory precision for each matrix, as required by European legislation.

Liquid Chromatographic Determination of Quinine, Hydroquinine, Saccharin, and Sodium Benzoate in Quinine Beverages

1985 ◽  
Vol 68 (4) ◽  
pp. 782-784
Author(s):  
Leonard P Valenti
Keyword(s):  
Sodium Benzoate ◽  
Direct Injection ◽  
Chromatographic Determination ◽  
Peak Height ◽  
Relative Standard ◽  
Sodium Saccharin ◽  
Liquid Chromatographic Determination ◽  
Liquid Chromatographic ◽  
The Relationship

Abstract A liquid chromatographic (LC) method is described for the determination of quinine, hydroquinine, sodium saccharin, and sodium benzoate in soft drinks. The method involves simple sample preparation, direct injection onto an octadecylsilane column, and elution with a methanol-acetonitrile-water-acetic acid (20 + 10 + 70 + 1) mobile phase. Eluted constituents are measured spectrophotometrically at 254 nm. The relationship between peak height and concentration was linear between 20 and 120 μg/mL for quinine. A relative standard deviation of 0.82% was obtained for commercial samples spiked with quinine, and the average recovery was 100.3%. The proposed procedure is accurate and rapid and can also detect hydroquinine (a natural contaminant of quinine), sodium saccharin, and sodium benzoate. Linear responses ranged from 0.45 to 20 (xg/mL for hydroquinine, from 54.8 to 219 μg/mL for sodium saccharin, and from 10.1 to 145.1 (ig/mL for sodium benzoate. The reproducibility of the LC method was evaluated with standard solutions of hydroquinine, sodium saccharin, and sodium benzoate, which produced relative standard deviations of 0.42, 0.46, and 1.13%, respectively. The average recoveries for sodium saccharin and sodium benzoate from spiked samples were 99.4 and 100.2%, respectively.

Liquid Chromatographic Determination of Sulfentrazone in Soil

1999 ◽  
Vol 82 (5) ◽  
pp. 1214-1216 ◽  
Author(s):  
G Anthony Ohmes ◽  
Thomas C Mueller
Keyword(s):  
Chromatographic Determination ◽  
Soil Samples ◽  
Ultraviolet Detection ◽  
Liquid Chromatographic Separation ◽  
Relative Standard ◽  
Limit Of Quantitation ◽  
Liquid Chromatographic Determination ◽  
Liquid Chromatographic ◽  
Soluble Components

Abstract A rapid method for the determination of sulfentrazone in soils is described. The method consists of extraction of soil samples with methanol, filtration, liquid chromatographic separation of methanol-soluble components through a C18 column, and ultraviolet detection at 220 nm. Recoveries from fortified surface soils were >85% for sulfentrazone. Average relative standard deviations over the soils examined was 7.7%. A conservative lower limit of quantitation for sulfentrazone was 40 ng/g soil.

Liquid Chromatographic Determination of Carminic Acid in Yogurt

1989 ◽  
Vol 72 (2) ◽  
pp. 231-234 ◽  
Author(s):  
Mercedes Jalón ◽  
Majesús Peńa ◽  
Julián C Rivas
Keyword(s):  
Chromatographic Determination ◽  
Carminic Acid ◽  
Reverse Phase ◽  
Liquid Chromatographic Method ◽  
Relative Standard ◽  
Array Detection ◽  
Liquid Chromatographic Determination ◽  
Phase Liquid ◽  
Liquid Chromatographic

Abstract A reverse-phase liquid chromatographic method is described for the determination of carminic acid in yogurt. A C18 column is used with acetonitrile-1.19M formic acid (19 + 81) as mobile phase and diode array detection. Sample preparation includes deproteinization with papain and purification in a polyamide column. The relative standard deviation for repeated determinations of carminic acid in a commercial strawberry-flavored yogurt was 3.0%. Recoveries of carminic acid added to a natural-flavored yogurt ranged from 87.2 to 95.3% with a mean of 90.2%. The method permits measurement of amounts as low as 0.10 mg/kg.

Gas-Liquid Chromatographic Determination of Total Inorganic Iodine in Milk

1977 ◽  
Vol 60 (6) ◽  
pp. 1307-1309 ◽  
Author(s):  
Hendrik J Bakker
Keyword(s):  
Liquid Chromatography ◽  
Iodine Content ◽  
Chromatographic Determination ◽  
Electron Capture Detection ◽  
Gas Liquid Chromatography ◽  
Relative Standard ◽  
Liquid Chromatographic Determination ◽  
Inorganic Iodine ◽  
Liquid Chromatographic

Abstract Total inorganic iodine in milk is determined by conversion to iodobutanone, which is quantitated by gas-liquid chromatography and electron capture detection. As little as 10 μg/L can be determined. The thyroid-active iodine content of milk can be determined rapidly with a relative standard deviation of 1.9%. Average recoveries for added iodide and iodine were 95.5 and 94.6%, respectively.

scholarly journals Simultaneous Determination of Chlorpheniramine Maleate and Dexamethasone in a Tablet Dosage Form by Liquid Chromatography

2005 ◽  
Vol 88 (6) ◽  
pp. 1677-1683 ◽  
Author(s):  
María A Moyano ◽  
María A Rosasco ◽  
María T Pizzorno ◽  
Adriana I Segall
Keyword(s):  
Potassium Phosphate ◽  
Reversed Phase ◽  
Chlorpheniramine Maleate ◽  
Constant Flow Rate ◽  
Liquid Chromatographic Method ◽  
Relative Standard ◽  
Tablet Dosage ◽  
Liquid Chromatographic ◽  
Interday Precision

Abstract An accurate, simple, reproducible, and sensible liquid chromatographic method was developed and validated for the determination of chlorpheniramine maleate and dexamethasone in a tablet formulation. The analysis was performed at room temperature on a reversed-phase C18 column with UV detection at 254 nm. The mobile phase consisted of 7.5 mM monobasic potassium phosphate in methanol–water (62.5 + 37.5) at a constant flow rate of 1 mL/min. The method was validated in terms of linearity, precision, accuracy, and specificity by forced decomposition of chlorpheniramine maleate and dexamethasone initiated by using acid, base, water, hydrogen peroxide, heat, and light. The response was linear in the ranges of 0.04–0.12 and 0.006–0.016 mg/mL for chlorpheniramine maleate (r2 = 0.9999) and dexamethasone (r2 = 0.9994), respectively. The relative standard deviation values for intra- and interday precision studies were 2.39 and 2.02, respectively, for chlorpheniramine maleate and 2.39 and 1.25, respectively, for dexamethasone. Recoveries ranged from 95.07 to 101.95% for chlorpheniramine maleate and from 97.75 to 102.10% for dexamethasone.

scholarly journals Liquid Chromatographic Determination of Acriflavine and Proflavine Residues in Channel Catfish Muscle

1997 ◽  
Vol 80 (3) ◽  
pp. 486-490
Author(s):  
Steven M Plakas ◽  
Kathleen R El Said ◽  
Edward L E Jester ◽  
F Aladar Bencsath ◽  
William L Hayton
Keyword(s):  
Channel Catfish ◽  
Solid Phase ◽  
Water Concentration ◽  
Chromatographic Determination ◽  
Relative Standard ◽  
Waterborne Exposure ◽  
Liquid Chromatographic Determination ◽  
Liquid Chromatographic ◽  
Poor Absorption

Abstract A liquid chromatographic (LC) method was developed for determination of acriflavine (ACR) and proflavine (PRO) residues in channel catfish muscle. Residues were extracted with acidified methanol solution, and extracts were cleaned up with Ci& solid-phase extraction columns. Residue concentrations were determined on an LC cyano column, with spectrophotometric detection at 454 nm. Catfish muscle was individually fortified with ACR (purified from commercial product) and PRO at concentrations of 5,10,20, 40, and 80 ppb (5 replicates per level). Mean recoveries from fortified muscle at each level ranged from 86 to 95%, with relative standard deviations (RSDs) of 2.5 to 5.7%. The method was applied to incurred residues of ACR and PRO in muscle after waterborne exposure of channel catfish to commercial acriflavine (10 ppm total dye for 4 h). RSDs for incurred residues of ACR and PRO were in the same range as those for fortified muscle. Low residue concentrations (<1 % of exposure water concentration) suggested poor absorption of ACR and PRO in catfish.

Liquid Chromatographic Determination of Morphine Sulfate and Some Contaminants in Injections and Bulk Drug Material: Collaborative Study

1988 ◽  
Vol 71 (5) ◽  
pp. 1046-1048
Author(s):  
Ada C Bello ◽  
Rita K Jhangiani
Keyword(s):  
Collaborative Study ◽  
Chromatographic Determination ◽  
Morphine Sulfate ◽  
System Suitability ◽  
Liquid Chromatographic Method ◽  
Relative Standard ◽  
Liquid Chromatographic Determination ◽  
Bulk Drug ◽  
Liquid Chromatographic

Abstract A liquid chromatographic method for the assay of morphine sulfate and some preservatives and impurities in the bulk drug and in injections has been developed and collaboratively studied in 8 laboratories. Each collaborator analyzed 5 samples: 1 bulk drug, 3 different concentrations of injectable dosages, and 1 prepared mixture containing, in addition to morphine sulfate, phenol, 2-mercaptobenzothiazole, and pseudomorphine. The proposed method quantitates morphine sulfate and resolves the other components for identification using a Clg reverse-phase column with a mobile solvent containing 240 mL methanol, 720 mL 0.005M 1-heptanesulfonic acid Na salt, and 10 mL acetic acid. Samples are prepared by direct dilution with mobile solvent minus 1-heptanesulfonic acid. All collaborators met system suitability requirements and performed the analysis without difficulty. No outliers were found when data were analyzed by the Dixon, Grubbs, double Grubbs, and Cochran tests. Relative standard deviations between laboratories (RSDR) for duplicate determinations of morphine sulfate ranged from 1.4 to 2.1%. Mean morphine sulfate recoveries for the bulk drug and the prepared mixture were 100.8 and 100.4%, respectively. The method has been approved interim official first action.

High Performance Liquid Chromatographic Determination of Primidone in Tablets

1982 ◽  
Vol 65 (5) ◽  
pp. 1063-1065
Author(s):  
Stanley E Roberts
Keyword(s):  
Mobile Phase ◽  
High Performance ◽  
Chromatographic Determination ◽  
High Performance Liquid Chromatographic ◽  
Hplc Method ◽  
Average Recovery ◽  
Relative Standard ◽  
Liquid Chromatographic Determination ◽  
Liquid Chromatographic

Abstract A high performance liquid chromatographic (HPLC) method is described for the quantitative determination of primidone in tablets. A ground tablet sample is diluted directly in the mobile phase, at a concentration of about 1 mg/mL of primidone, mixed and deaerated, and filtered. The resulting solution is then quantitated by HPLC. The average spike recoveries for the 50 mg and 250 mg tablets were 101.2% and 99.0%, respectively. The average recovery for an authentic mixture formulated at the 250 mg level was 100.1% with a relative standard deviation of 0.45%.

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