Liquid Chromatographic Determination of Strychnine in Stomach Contents
Keyword(s):
Abstract A chloroform extract of stomach contents at basic pH is concentrated and then extracted with 0.1M phosphoric acid. The acid extract is chromatographed on a 10 cm reverse phase column, using 0.005M phosphate buffer (pH 3.0)-acetonitrile-tetrahydrofuran (750 + 135 + 115) containing 0.01M octanesulfonic acid at a flow rate of 1.0 raL/ min for elution. Strychnine eluted in 7.3 min. Recoveries from spiked stomach contents averaged 92%. The method can be used without modification for other alkaloids.
1990 ◽
Vol 73
(6)
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pp. 893-895
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1984 ◽
Vol 67
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pp. 861-862
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1996 ◽
Vol 319
(1-2)
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pp. 187-190
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