Spectrofluorometric Assay of Thiamine Hydrochloride in Injections and Multivitamin
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Abstract A spectrofluorometric method is described for the determination of thiamine hydrochloride. The method is based on formation of a fluorescent product by oxidation of thiamine HC1 with 2,3,5,6-tetrabromo- l,4-benzoquinone in aqueous acetonitrile solution. The reaction product is stable for at least 6 h and shows excitation and emission maxima around 355 and 420 nm, respectively. The method is highly selective for thiamine HC1 in the presence of other B vitamins. Thiamine HC1 can be determined at concentrations as low as 20-160 ng/ mL of the final solutions. When the method was applied to the determination of thiamine HC1 added to some commercially available multivitamin preparations, recoveries were 98.78-99.98%
DETERMINATION OF THIAMINE HCL (VITAMIN B1) AND PYRIDOXINE HCL (VITAMIN B6) CONTENT IN TABLET BY FTIR
2016 ◽
Vol 8
(10)
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pp. 257
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2020 ◽
Vol 77
(5)
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pp. 699-705
2013 ◽
Vol 24
(7)
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pp. 60-73
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