scholarly journals Determination of Heterocyclic Aromatic Amines in Process Flavors by a Modified Liquid Chromatographic Method

1996 ◽  
Vol 79 (3) ◽  
pp. 813-816 ◽  
Author(s):  
Gracia A Perfetti
Keyword(s):  
Aromatic Amines ◽  
Chromatographic Method ◽  
Solid Phase ◽  
Extraction Procedure ◽  
Heterocyclic Aromatic Amines ◽  
Complex Process ◽  
Liquid Chromatographic Method ◽  
Low Levels ◽  
Liquid Chromatographic

Abstract A liquid chromatographic method for determining heterocyclic aromatic amines (HAAs) was modified so that it can be used to quantitate the following 5 HAAs in complex process flavors: 2-amino-3-methylimidazo[4,5-f]quinoline (IQ); 2-amino-3,4-dimethylimidazo[4,5-f]quinoline (MelQ); 2-amino-3,8-dimethylimidazo[4,5-f]quinoxaline (MelQx); 2-amino-3,4,8-trimethylimidazo[4,5-f]quinoxaline (diMelQx); and 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP). By changing the initial extraction procedure, PhIP recovery improved from 35 to 87%. An additional cleanup step using ion-exchange solid-phase extraction reduced the levels of interfering components, thus allowing determination of low levels of the amines. Recoveries from process flavors spiked at 25 ppb averaged 83–90%.

scholarly journals Determination of Nitroimidazoles and Their Metabolites in Swine Tissues by Liquid Chromatography

2003 ◽  
Vol 86 (3) ◽  
pp. 505-509 ◽  
Author(s):  
Jianzhong Shen ◽  
Yue Yhang ◽  
Suxia Zhang ◽  
Shuangyang Ding ◽  
Xinhua Xiang
Keyword(s):  
Chromatographic Method ◽  
Solid Phase ◽  
Liquid Extraction ◽  
Phase Extraction ◽  
Liquid Liquid Extraction ◽  
Liquid Chromatographic Method ◽  
Swine Tissue ◽  
Liquid Chromatographic ◽  
Sensitive Method

Abstract A liquid chromatographic method was developed for determination of metronidazole (MNZ), ronidazole (RNZ), dimetridazole (DMZ), and 2-hydroxymethyl-1-methyl-5-nitroimidazole (DMZOH) in swine tissue. After extraction with ethyl acetate and evaporation, the nitroimidazoles were redissolved in hydrochloric acid. Hexane was used in the liquid–liquid extraction to remove fat. An Oasis® HLB solid-phase extraction was performed after neutralization of the acidic extract. The limits of detection were 1.0–2.0 μg/kg for DMZOH, MNZ, RNZ, and DMZ in muscle and liver. Average recoveries ranged from 80.1 to 83.9% in muscle fortified at 10, 20, and 50 μg/kg; average re-coveries in liver ranged from 78.9 to 82.3%. The procedure provides a simple and sensitive method for monitoring DMZOH, MNZ, RNZ, and DMZ residues in swine tissues.

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