Determination of Avilamycin in Poultry Feeds by Liquid Chromatography

1999 ◽  
Vol 82 (3) ◽  
pp. 579-585 ◽  
Author(s):  
Charles A Scott ◽  
Durand W Yordy ◽  
Mark R Coleman
Keyword(s):  
Liquid Chromatography ◽  
Solid Phase ◽  
Reversed Phase ◽  
Reversed Phase Liquid Chromatography ◽  
Poultry Feeds ◽  
Matrix Interferences ◽  
Relative Standard ◽  
Phase Liquid ◽  
Feed Samples

Abstract Avilamycin was extracted from feed with acetonitrile. Isolation of avilamycin factors from feed matrix interference was accomplishedby normal phase solid-phase extraction with silica as sorbent. Reversed-phase liquid chromatography was subsequently used to separate and quantitate the primary biologically activefactors A and B for determination of chemical potency. This method combines specificity for avilamycins A and B in poultry feeds with simple sample preparation that removes matrix interferences. Recoveries of factor A ranged from 93.29 to 97.26%, with precision (relative standard deviation) ranging from 1.1to 3.4%. Avilamycin factors in feed samples tested ranged from 4.45 to 17.82 μg/g for factor A and from 0.80 to 3.18 μg/g for factor B.

Determination of Polynuclear Aromatic Hydrocarbons in Seafood by Liquid Chromatography with Fluorescence Detection

1992 ◽  
Vol 75 (5) ◽  
pp. 872-877 ◽  
Author(s):  
Gracia A Perfetti ◽  
Patricia J Nyman ◽  
Sheryl Fisher ◽  
Frank L Joe ◽  
Gregory W Diachenko
Keyword(s):  
Liquid Chromatography ◽  
Fluorescence Detection ◽  
Aromatic Hydrocarbons ◽  
Solid Phase ◽  
Reversed Phase ◽  
Polynuclear Aromatic Hydrocarbons ◽  
Matrix Interferences ◽  
Phase Liquid ◽  
Certified Values

Abstract Modification of a previously published method for determination of polynuclear aromatic hydrocarbons (PAHs) produces very clean seafood extracts in less than half the time. After alkaline digestion of the seafood, PAHs were partitioned into 1,1,2- trichlorotrifluoroethane. The resulting extract was cleaned up by solid-phase extraction on alumina, silica, and C18 adsorbents and then analyzed by gradient reversed-phase liquid chromatography with programmable fluorescence detection. Average recoveries of 12 PAHs [acenaphthene, anthracene, fluoranthene, pyrene, benz(a)anthracene, chrysene, benzo(b)fluoranthene, benzo(k)- fluoranthene, benzo(a)pyrene, dibenz(a,h)anthracene, benzo(ghi)perylene, and indeno(1,2,3-cd)pyrene] from 5 different matrixes (mussels, oysters, clams, crabmeat, and salmon) spiked at low partsper- billion levels ranged from 76 to 94%. Estimated limits of quantitation ranged from 0.01 to 0.6 ppb PAHs in extracts that were free of matrix interferences. Results of analyses of a mussels standard reference material obtained from the National Institute of Standards and Technology were in good agreement with the certified values.

scholarly journals Determination of Tylosin in Feeds by Liquid Chromatography with Solid-Phase Extraction

2004 ◽  
Vol 87 (2) ◽  
pp. 341-345 ◽  
Author(s):  
Matthew J Gramse ◽  
Paul E Jacobson ◽  
James C Selkirk
Keyword(s):  
Liquid Chromatography ◽  
Solid Phase Extraction ◽  
Solid Phase ◽  
Reversed Phase ◽  
Phase Extraction ◽  
Column Temperature ◽  
Solid Phase Extraction Cartridge ◽  
Relative Standard ◽  
Phase Liquid

Abstract A method was developed for the determination of tylosin in feeds. The method involves extraction of tylosin with methanol, concentration under a stream of nitrogen, and cleanup using Phenomenex C18 solid-phase extraction cartridge. Analyte separation and quantitation were achieved by gradient reversed-phase liquid chromatography and UV absorbance at 285 nm with a reference wavelength of 320 nm with column temperature of 45°C. Average spike recoveries for samples prepared at 4 spiking levels (22.7, 181, 907, and 1000 g/ton) were 111.0, 94.9, 96.2, and 98.6%, respectively. The overall method precision at each of the 4 spiking levels was ≤ 7.85% relative standard deviation. The limits of detection and quantitation (g/ton) were 2.16 and 7.20 g/ton, respectively.

Sensitive and Rapid Reversed-Phase Liquid Chromatography–Fluorescence Method for Determining Bisphenol A Diglycidyl Ether in Aqueous–Based Food Simulants

1991 ◽  
Vol 74 (6) ◽  
pp. 925-928 ◽  
Author(s):  
P Paseiro Losada ◽  
P López Mahia ◽  
L Vázquez Odériz ◽  
J Simal Lozano ◽  
J Simal Gándara
Keyword(s):  
Liquid Chromatography ◽  
Bisphenol A ◽  
Reversed Phase ◽  
Diglycidyl Ether ◽  
Reversed Phase Liquid Chromatography ◽  
Food Simulants ◽  
Bisphenol A Diglycidyl Ether ◽  
Relative Standard ◽  
Phase Liquid

Abstract A method has been developed for determination of bisphenol A diglycidyl ether (BADGE) in 3 aqueous-based food simulants: water, 15% (v/v) ethanol, and 3% (w/v) acetic acid. BADGE is extracted with C18 cartridges and the extract Is concentrated under a stream of nitrogen. BADGE is quantltated by reversed-phase liquid chromatography with fluorescence detection. Relative precision at 200 μg/L was 3.4%, the detection limit of the method was 0.1 μg/L, and recoveries of spiking concentrations from 1 to 8 μg/L were nearly 100%. Relative standard deviations for the method ranged from 3.5 to 5.9%, depending on the identity of the spiked aqueousbased food simulant.

Determination of Roxarsone in Feeds Using Solid Phase Extraction and Liquid Chromatography With Ultraviolet Detection

1993 ◽  
Vol 76 (5) ◽  
pp. 956-961 ◽  
Author(s):  
Robert E Sapp ◽  
Sandra Davidson
Keyword(s):  
Liquid Chromatography ◽  
Solid Phase Extraction ◽  
Solid Phase ◽  
Reversed Phase ◽  
Poultry Feed ◽  
Phase Extraction ◽  
Reversed Phase Liquid Chromatography ◽  
Field Samples ◽  
Phase Liquid

Abstract A method is presented for detection and quantitation of Roxarsone in poultry feed by liquid chromatography. The drug is extracted by phosphate buffer and determined by solid phase extraction and reversed-phase liquid chromatography. Recoveries of the sample spikes and fortified field samples agree closely with those obtained by the standard spectrophotometric method.

Determination of Tylosin and Tilmicosin Residues in Animal Tissues by Reversed-Phase Liquid Chromatography

1994 ◽  
Vol 77 (2) ◽  
pp. 331-333 ◽  
Author(s):  
Wayne Chan ◽  
Geoff C Gerhardt ◽  
Craig D C Salisbury
Keyword(s):  
Liquid Chromatography ◽  
Solid Phase Extraction ◽  
Solid Phase ◽  
Reversed Phase ◽  
Phase Extraction ◽  
Animal Tissues ◽  
Reversed Phase Liquid Chromatography ◽  
Tissue Extracts ◽  
Phase Liquid

Abstract A method for the simultaneous determination of ty-losin and tilmicosin residues in animal tissues is reported. Solid-phase extraction columns are used to isolate the drugs from tissue extracts. Determination is accomplished by reversed-phase liquid chromatography with UV detection at 287 nm. Mean recoveries from spiked tissues were 79.9% (coefficient of variation [CV], 8.1%) for tylosin and 92.6% (CV, 8.7%) for tilmicosin. Detection limits for tylosin and tilmicosin were 0.020 and 0.010 ppm, respectively.

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