scholarly journals Validation Study of a Rapid ELISA for Detection of Deoxynivalenol in Wheat, Barley, Malted Barley, Corn, Oats, and Rice

2010 ◽  
Vol 93 (2) ◽  
pp. 600-610 ◽  
Author(s):  
Anthony Lupo ◽  
Chris Roebuck ◽  
Ken Settimo ◽  
Anna Quain ◽  
Justina Kennedy ◽  
...  
Keyword(s):  
Rapid Method ◽  
Electron Capture ◽  
Validation Study ◽  
Reference Method ◽  
Elisa Test ◽  
Electron Capture Detection ◽  
Reference Methods ◽  
Test Kit ◽  
Aoac Official ◽  
Research Institute

Abstract Neogen Corp. developed the Veratox DON test kit for the detection of deoxynivalenol (DON). The purpose of this study was to validate the method under the requirements of the AOAC Research Institute Performance Tested MethodsSM (PTM) program. There are two AOAC Official MethodsSM for DON detection: 986.17 and 986.18, the first of which is a TLC method and the second a GC method. A rapid method (PTM 000701) has also been performance tested by the AOAC Research Institute. One of the most widely used reference methods; however, is a GC method with electron capture detection that is referred to as the reference method in this paper. Although considered the reference method, the GC procedure is complicated and requires the investment of both expensive equipment and a highly skilled technician. A rapid (e.g., ELISA) test kit needs to be validated by the AOAC Research Institute. The Veratox 2/3 method is highly reproducible with average CV values <10, and is very accurate, showing >97 correlation to reference methods.

scholarly journals Validation Study of a Rapid ELISA for Detection of Aflatoxin in Corn

2010 ◽  
Vol 93 (2) ◽  
pp. 587-599 ◽  
Author(s):  
Anthony Lupo ◽  
Chris Roebuck ◽  
Monica Dutcher ◽  
Justina Kennedy ◽  
Mohamed Abouzied
Keyword(s):  
Rapid Method ◽  
Validation Study ◽  
Reference Method ◽  
Elisa Test ◽  
Hplc Method ◽  
Total Aflatoxin ◽  
Expensive Equipment ◽  
Test Kit ◽  
Aoac Official ◽  
Research Institute

Abstract Neogen Corp. developed the Veratox aflatoxin test kit for the detection of total aflatoxin. The purpose of this study was to validate the method under the requirements of the AOAC Research Institute Performance Tested MethodsSM (PTM) program. There are several AOAC Official MethodsSM for total aflatoxin detection in corn (994.08, 990.33, 979.18, 993.17, 990.32, 993.16, 991.31, and 990.74), varying between rapid and analytical-based methods and one rapid method that has been performance tested by the AOAC Research Institute (PTM 030701). However, the widely used reference method is AOAC Official MethodSM994.08, which is an HPLC method and is referred to as the reference method in this paper. Although considered the reference method, the HPLC procedure is complicated and requires the investment of both expensive equipment and a highly skilled technician. A rapid (e.g., ELISA) test kit to be validated by the AOAC Research Institute is needed.

scholarly journals Validation Study of a Rapid ELISA for Detection of Histamine in Tuna

2011 ◽  
Vol 94 (3) ◽  
pp. 886-899 ◽  
Author(s):  
Anthony Lupo ◽  
Mark Mozola
Keyword(s):  
Validation Study ◽  
Reference Method ◽  
Elisa Test ◽  
Elisa Method ◽  
Fluorometric Method ◽  
Test Kit ◽  
Aoac Official ◽  
Research Institute

Abstract Neogen Corp. has developed an improved Veratox histamine test kit for the detection of histamine in tuna tissue. The purpose of this study was to validate the method under the requirements of AOAC Research Institute (RI) Performance Tested MethodsSM. Three AOAC Official MethodsSM for histamine (954.04, 957.07, and 977.13) and one ELISA method have been performance tested by the AOAC RI. The most popular is AOAC Official MethodSM977.13, the fluorometric method, which is considered the reference method, but is complicated and time-consuming. The need for a rapid ELISA test kit to be validated by the AOAC RI exists.

scholarly journals In-House Validation Study of the DuPont Qualicon BAX System Q7 Instrument with the BAX System PCR Assay for Salmonella (Modification of AOAC Official MethodSM 2003.09 and AOAC Research Institute Performance-Tested MethodSM 100201)

2009 ◽  
Vol 92 (3) ◽  
pp. 989-994 ◽  
Author(s):  
George Tice ◽  
Bridget Andaloro ◽  
H Kirk White ◽  
Lance Bolton ◽  
Siqun Wang ◽  
...  
Keyword(s):  
Validation Study ◽  
Reference Method ◽  
Pcr Assay ◽  
Paired Samples ◽  
Reference Methods ◽  
Comparable Performance ◽  
Aoac Official ◽  
Inspection Service ◽  
Positive Results ◽  
The U.S

Abstract In 2006, DuPont Qualicon introduced the BAX<sup/> system Q7 instrument for use with its assays. To demonstrate the equivalence of the new and old instruments, a validation study was conducted using the BAX system PCR Assay for Salmonella, AOAC Official MethodSM 2003.09, on three food types. The foods were simultaneously analyzed with the BAX system Q7 instrument and either the U.S. Food and Drug Administration Bacteriological Analytical Manual or the U.S. Department of AgricultureFood Safety and Inspection Service Microbiology Laboratory Guidebook reference method for detecting Salmonella. Comparable performance between the BAX system and the reference methods was observed. Of the 75 paired samples analyzed, 39 samples were positive by both the BAX system and reference methods, and 36 samples were negative by both the BAX system and reference methods, demonstrating 100 correlation. Inclusivity and exclusivity for the BAX system Q7 instrument were also established by testing 50 Salmonella strains and 20 non-Salmonella isolates. All Salmonella strains returned positive results, and all non-Salmonella isolates returned a negative response.

Validation Study of the Veratox R5 Rapid ELISA for Detection of Gliadin

2013 ◽  
Vol 96 (1) ◽  
pp. 121-132 ◽  
Author(s):  
Anthony Lupo ◽  
Chris Roebuck ◽  
Aaron Walsh ◽  
Mark Mozola ◽  
Mohamed Abouzied
Keyword(s):  
Monoclonal Antibody ◽  
Validation Study ◽  
Rapid Test ◽  
Codex Alimentarius ◽  
Test Kit ◽  
Aoac Official ◽  
Research Institute

Abstract Neogen Corp. developed the Veratox R5 Gliadin test kit for the detection of gliadin based on the monoclonal antibody R5 developed by Enrique Mendez (1). The purpose of this study was to validate the method under the requirements of the AOAC Research Institute Performance Tested MethodSM program. There are two AOAC Official MethodsSM for gluten detection in foods, 991.19 and 2012.01 (2), both of which are ELISAs. With the R5 Mendez method listed in the CODEX Alimentarius as a type 1 method for the detection of gluten in foods, the need to have additional rapid test kits validated by the AOAC Research Institute exists.

scholarly journals Modification of the BAX®Salmonella Test Kit to Include a Hot Start Functionality (Modification of AOAC Official MethodSM 2003.09)

2011 ◽  
Vol 94 (5) ◽  
pp. 1490-1505
Author(s):  
F Morgan Wallace ◽  
Deana DiCosimo ◽  
Andrew Farnum ◽  
George Tice ◽  
Bridget Andaloro ◽  
...  
Keyword(s):  
Reference Method ◽  
Department Of Agriculture ◽  
Reference Methods ◽  
Hot Start ◽  
System Method ◽  
Test Kit ◽  
Aoac Official ◽  
Inspection Service ◽  
Identical Performance ◽  
The U.S

Abstract In 2010, the BAX® System PCR assay for Salmonella was modified to include a hot start functionality designed to keep the reaction enzyme inactive until PCR begins. To validate the assay's Official Methods of Analysis status to include this procedure modification, an evaluation was conducted on four food types that were simultaneously analyzed with the BAX System and either the U.S. Food and Drug Administration's Bacteriological Analytical Manual or the U.S. Department of Agriculture-Food Safety and Inspection Service Microbiology Laboratory Guidebook reference method for detecting Salmonella. Identical performance between the BAX System method and the reference methods was observed. Additionally, lysates were analyzed using both the BAX System Classic and BAX System Q7 instruments with identical results using both platforms for all samples tested. Of the 100 samples analyzed, 34 samples were positive for both the BAX System and reference methods, and 66 samples were negative by both the BAX System and reference methods, demonstrating 100% correlation. No instrument platform variation was observed. Additional inclusivity and exclusivity testing using the modified test kit demonstrated the test kit to be 100% accurate in evaluation of test panels of 352 Salmonella strains and 46 non-Salmonella strains.

scholarly journals Validation Study of Immunoaffinity Column Chromatography Coupled with Solution Fluorometry or HPLC for the Detection of Aflatoxin in Peanuts and Corn

2011 ◽  
Vol 94 (2) ◽  
pp. 572-588 ◽  
Author(s):  
Anthony Lupo ◽  
Anna Quain ◽  
Alan Fitzsimmons ◽  
Andrew Allan ◽  
Bert Popping ◽  
...  
Keyword(s):  
Validation Study ◽  
Reference Method ◽  
Reference Value ◽  
Immunoaffinity Column ◽  
Total Aflatoxin ◽  
Rapid Methods ◽  
Relative Recovery ◽  
Aoac Official ◽  
The Mean ◽  
Research Institute

Abstract Neogen Corp. has developed the Neocolumn for Aflatoxin DR for the detection of total aflatoxin by HPLC or solution fluorometry. The purpose of this study was to validate the method under the requirements of the AOAC Research Institute Performance Tested MethodsSM (PTM) program. There are several AOAC Official MethodsSM for detection of total aflatoxin in corn; they consist of rapid and analytical-based methods and two rapid methods (PTMs 030701 and 050901) that have been performance tested by the AOAC Research Institute. A widely used reference method, however, is AOAC Official MethodSM 991.31, which uses immumoaffinity cleanup followed by HPLC or solution fluorometry and is referred to as the reference method in this document. In internal studies, the Neocolumn method coupled with solution fluorometry demonstrated a relative recovery from peanuts of 101.6 of the reference value, with a CV of 3.9 across all levels analyzed; when coupled with HPLC, the Neocolumn method demonstrated a relative recovery from peanuts of 103.0 of the reference value with a CV of 3.5 across all levels analyzed. The Neocolumn method coupled with solution fluorometry demonstrated a relative recovery from corn of 116.9 of the reference value with a CV of 6.1 across all levels analyzed; when coupled with HPLC, the Neocolumn method demonstrated a relative recovery from corn of 91.2 of the reference value, with a CV of 5.4 across all levels analyzed. Calculations were made by comparison with the mean result obtained by the HPLC reference method, which showed respective CV values of 3.9 and 2.0 for recoveries from peanuts and corn, respectively.

scholarly journals Comparison of duplicate portion and 24 h recall as reference methods for validating a FFQ using urinary markers as the estimate of true intake

2015 ◽  
Vol 114 (8) ◽  
pp. 1304-1312 ◽  
Author(s):  
Laura Trijsburg ◽  
Jeanne H. M. de Vries ◽  
Hendriek C. Boshuizen ◽  
Paul J. M. Hulshof ◽  
Peter C. H. Hollman ◽  
...  
Keyword(s):  
Measurement Error ◽  
Validation Study ◽  
Reference Method ◽  
Bias Error ◽  
Correlated Errors ◽  
Web Based ◽  
Urinary Markers ◽  
Reference Methods ◽  
Duplicate Portion ◽  
Better Than

AbstractAs FFQ are subject to measurement error, associations between self-reported intake by FFQ and outcome measures should be adjusted by correction factors obtained from a validation study. Whether the correction is adequate depends on the characteristics of the reference method used in the validation study. Preferably, reference methods should (1) be unbiased and (2) have uncorrelated errors with those in the FFQ. The aim of the present study was to assess the validity of the duplicate portion (DP) technique as a reference method and compare its validity with that of a commonly used reference method, the 24 h recall (24hR), for protein, K and Na using urinary markers as the unbiased reference method. For 198 subjects, two DP, two FFQ, two urinary biomarkers and between one and fifteen 24hR (web based and/or telephone based) were collected within 1·5 years. Multivariate measurement error models were used to estimate bias, error correlations between FFQ and DP or 24hR, and attenuation factors of these methods. The DP was less influenced by proportional scaling bias (0·58 for protein, 0·72 for K and 0·52 for Na), and correlated errors between DP and FFQ were lowest (protein 0·28, K 0·17 and Na 0·19) compared with the 24hR. Attenuation factors (protein 0·74, K 0·54 and Na 0·43) also indicated that the DP performed better than the 24hR. Therefore, the DP is probably the best available reference method for FFQ validation for nutrients that currently have no generally accepted recovery biomarker.

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