519 Late-Breaking: Evaluation of the 7 & 7 Synch and Bee Synch II protocols for control of the estrous cycle among cows of varying percentage Bos indicus influence

2021 ◽  
Vol 99 (Supplement_3) ◽  
pp. 154-155
Author(s):  
Carson M Andersen ◽  
Matthew P Holton ◽  
Pedro L Fontes ◽  
Jordan M Thomas ◽  
Lucas Goncalves ◽  
...  
Keyword(s):  
Estrous Cycle ◽  
Artificial Insemination ◽  
Color Doppler ◽  
Ovarian Follicle ◽  
Bos Indicus ◽  
Prostaglandin F2α ◽  
Blood Perfusion ◽  
Fixed Time ◽  
Gonadotropin Releasing Hormone ◽  
Corpora Lutea

Abstract The 7 & 7 Synch and Bee Synch II protocols for control of the estrous cycle were compared among cows with varying percentages of Bos indicus influence. Angus (n = 48), ⅛ - ¼ Brahman-influenced (n = 90), Brangus (n = 11), and ½ Brahman-influenced (n = 56) cows were blocked based on breed, age, and days postpartum and randomly assigned to treatment. Cows treated with 7 & 7 Synch (n = 104) received prostaglandin F2α (PG; 25 mg dinoprost tromethamine) and insertion of an intravaginal progesterone-releasing insert (CIDR; 1.38g progesterone) on Day -17, gonadotropin-releasing hormone (GnRH; 100 μg gonadorelin) on Day -10, and PG coincident with CIDR removal on Day -3. Cows treated with Bee Synch II (n = 101) received PG coincident with CIDR insertion on Day -8 and PG coincident with CIDR removal on Day -3. Fixed-time artificial insemination was performed on Day 0 at 66 h. Ovarian ultrasound was performed on Day -3 to assess presence of corpora lutea (CL) and diameter of the largest ovarian follicle (LFD), and color Doppler was used on Day 7 to assess CL blood perfusion. A greater proportion of 7 & 7 Synch cows (P < 0.0001) had CL on Day -3 (83%; 86/104) compared with Bee Synch II cows (48%; 48/101). On Day -3, LFD did not differ among Bos indicus-influenced cows treated with Bee Synch II (12.5±0.3 mm), Bos indicus-influenced cows treated with 7 & 7 Synch (12.2±0.3 mm), or Angus cows treated with 7 & 7 Synch (12.1±0.6 mm); however, greater LFD was observed among Angus cows treated with Bee Synch II (15.4±0.8 mm). Brangus cows had greater periphery signal percentages (P = 0.02) when treated with 7 & 7 Synch compared with Bee Synch II. In conclusion, across cow of varying percentage Bos indicus influence, 7 & 7 Synch resulted in a greater proportion of cows with CL on Day -3.

Estrous synchronization and fertility of cross-bred dairy cows using prostaglandin F2α and gonadotropin releasing hormone

2021 ◽  
pp. 171-175
Author(s):  
Abubakr O. Ismail ◽  
◽  
Erneo B. Ochi ◽  
Ambrose S. Jubara ◽  
Bakri Ahmed A/Rahim ◽  
...  
Keyword(s):  
Dairy Cows ◽  
Artificial Insemination ◽  
Prostaglandin F2α ◽  
Fixed Time ◽  
Gonadotropin Releasing Hormone ◽  
Pregnancy Rates ◽  
Releasing Hormone ◽  
Treatment Groups ◽  
Estrous Synchronization ◽  
Descriptive Statistical Analysis

A comparative hormonal study of prostaglandin, gonadotropin releasing hormone and their combination was conducted on 30 randomly selected cross-bred dairy cows of SEMEX project for artificial insemination in Hillat Kuku, Khartoum North, Sudan. The study attempts to determine the response of the animals to hormonal induction and synchronization of estrous as well as fertility following fixed time artificial insemination. Three treatment groups of 10 cows each were undertaken. Groups 1, 2 and 3 involved administration of prostaglandin F2α (PGF2α), gonadotropin releasing hormone (GnRH) and a combination of PGF2α + GnRH, respectively. Descriptive statistical analysis was performed. The results revealed that all the three protocols induced and synchronized estrous were almost synchronous in all the treated groups. However, protocol 3 revealed a significantly (p<0.05) better result of 70% pregnancy rate compared to other two protocols that equally provided 50% pregnancy rates. The study resolves that protocol 3 be highly recommended to change the mindset of farmers about the spread of reproductive technology in Sudan. Protocols 1 and 2 provided acceptable pregnancy rates that can be enhanced with the improvement of management.

17 INFLUENCE OF DIFFERENT CONCENTRATIONS OF PROGESTERONE DURING FIXED-TIME ARTIFICIAL INSEMINATION PROTOCOLS ON OVULATION AND PREGNANCY RATES IN NELORE HEIFERS

2008 ◽  
Vol 20 (1) ◽  
pp. 89
Author(s):  
M. F. Pegorer ◽  
R. L. Ereno ◽  
C. M. Barros
Keyword(s):  
Artificial Insemination ◽  
Bos Indicus ◽  
Prostaglandin F2α ◽  
Fixed Time ◽  
Estradiol Benzoate ◽  
Pregnancy Rates ◽  
High Concentration ◽  
Serum Progesterone ◽  
Significant Difference ◽  
Intravaginal Device

Previous reports indicate that a high concentration of serum progesterone (P4) decreases LH pulse frequency (Burke et al. 1996 Anim. Reprod. Sci. 45, 13–28) and, consequently, follicular growth. This may reduce the efficiency of fixed-time artificial insemination (FTAI) protocols that utilize P4 in Bos indicus heifers (Marques et al. 2005 Acta Sci. Vet.). The objective of the present work was to evaluate the influence of different P4 concentrations on the ovulation and pregnancy rates of zebu heifers subjected to hormonal protocols for FTAI. Four hormonal protocols with different P4 concentrations were used to induce synchronization of ovulation in Nelore (Bos indicus) heifers (n = 292). Before the beginning of the treatments, the animals were subjected to ovary evaluations by ultrasonography (Aloka SSD 500, 5-MHz probe; Aloka, Tokyo, Japan) twice in an interval of 10 days. Only heifers with a corpus luteum (CL) in at least one of the ultrasonographic evaluations were utilized. At a random stage of the estrous cycle, the selected heifers received an intravaginal progesterone-releasing device containing 0.558 g of P4 [Triu�, Biogenesis, Buenos Aires, Argentina; Treatment 1 (T1); n = 76], or 1 g of P4 [Cronipress�, Biogenesis; Treatment 2 (T2); n = 70], or 0.558 g of P4 and prostaglandin F2α (PGF2α) [150 �g d-cloprostenol, IM, Croniben�, Biogenesis; Treatment 3 (T3), n = 75], or 1 g of P4 and PGF2α [Treatment 4 (T4), n = 71]. At the time of intravaginal device insertion (Day 0 = D0), all animals received 2 mg of estradiol benzoate (EB, Bioestrogen�, IM, Biogenesis), and PGF2α was administered only in animals from groups T3 and T4. Eight days later (D8), PGF2α (0.150 g d-cloprostenol) was administered to all heifers and the P4 source was removed. Twenty-four h after removal of the intravaginal device, the heifers were treated with EB (1 mg, IM), and 30 to 36 h later all animals were fixed-time inseminated (FTAI, D10), without estrus detection. In a subset of heifers from each group (T1 = 28; T2 = 34; T3 = 33; T4 = 31), ovarian ultrasonography was performed on D9 (at 8:00 A.M.) and D11 (at 14:00 P.M.) in order to determine ovulation rate after each treatment. The data were analyzed by logistic regression (GENPROC, SAS; SAS Institute, Inc., Cary, NC, USA). Ovulation rates after treatments T1, T2, T3, and T4, were, respectively, 71.4% (20/28), 76.5% (26/34), 84.9% (28/33), and 70.9% (22/31), and there was no significant difference among groups (P > 0.05). Pregnancy rates, ascertained by ultrasonography 40 days after AI, were 30.2% (23/76), 25.7% (18/70), 33.3% (25/75), and 28.2% (20/71), respectively, for T1, T2, T3, and T4, and likewise there was no significant difference among groups (P > 0.05). It is concluded that reduction of progesterone concentration in the intravaginal device (1 g v. 0.558 g) associated or not with administration of PGF2α at the beginning of treatments did not significantly influence ovulation and pregnancy rates in Nelore heifers submitted to a FTAI protocol. Additional experiments are underway to confirm or not the present results. This work was supported by FAPESP (Brazil) and BIOGENESIS (Argentina).

13 USE OF EQUINE CHORIONIC GONADOTROPIN OR FOLLICLE-STIMULATING HORMONE-LUTEINIZING HORMONE AT IMPLANT REMOVAL OR AT FIXED-TIME ARTIFICIAL INSEMINATION TIME IN PREGNANCY RATE OF SUCKLED NELLORE COWS

2015 ◽  
Vol 27 (1) ◽  
pp. 99
Keyword(s):  
Pregnancy Rate ◽  
Artificial Insemination ◽  
Bos Indicus ◽  
Prostaglandin F2α ◽  
Fixed Time ◽  
Pregnancy Rates ◽  
Implant Removal ◽  
Transrectal Ultrasonography ◽  
Follicular Growth ◽  
The Difference

Use of eCG as an inducer of follicular growth has improved the efficacy of fixed-time artificial insemination (FTAI) protocols and increased the ovulatory responses and pregnancy rates in beef cattle with low body condition scores (BCS) or are recently postpartum and anestrous. However, there are other gonadotropins such as FSH in different commercial applications (Pluset® – FSH : LH proportion ~50.0%) that could be potentially used to increase follicular growth but with controversial results. The goal of this trial was to evaluate the effects of replacing eCG with FSH/LH in two moments on the pregnancy rates (PR) of lactating Bos indicus cows raised in native grassland at Pantanal. The cows were subjected to a synchronization-of-ovulation protocol and FTAI based on progesterone, oestradiol benzoate (EB), and prostaglandin F2α. On Day 0, Nellore multiparous cows (n = 352) at 42 days postpartum with BCS = 4.9 (1–9) were evaluated by transrectal ultrasonography and received progesterone implant (DIB 1.0 g of progesterone) plus 2 mg of EB IM. Devices were removed and prostaglandin F2α was injected on Day 8 with 1 mg of EB. At the time of implant removal, the animals received T1 – 1 mL of saline solution IM (control; n = 80); T2 – 300 IU IM of eCG (Novormon®; ECG; n = 92); T3 – 40 IU IM of FSH/LH (Pluset®; Pluset; n = 98). The T4-group cows received 40 IU IM of FSH/LH (Pluset®) at FTAI time (Pluset-FTAI; n = 82); cows were timed-AI on Day 10 (44–48 h after implant removal) and evaluated by transrectal ultrasonography to measure the preovulatory follicle (POF) at FTAI and to estimate the pregnancy rate on Day 45. The effects of the treatment, sire, and BCS on pregnancy rate were evaluated using PROC GLIMMIX (SAS Institute Inc., Cary, NC). Because there was no difference between BCS, sire, and PR, they were removed from model. Pregnancy rate was not different between the treatment groups (control: 38.70%; ECG: 51.08%; Pluset: 45.91%, and Pluset-FTAI: 39.02%; P > 0.05), but the difference was found in POF, higher in ECG group – 13.53 mm compared with others (Pluset: 12.79 mm; control: 11.73 mm, and Pluset-FTAI: 12.01 mm; P < 0.05). Although PR was not different between treatments, the data are in agreement with the size of POF, where the largest POF are associated with tendency of higher pregnancy rates in ECG group. In conclusion, commercial FSH solution does not provide increases in PR, and eCG increases the preovulatory follicles in Nellore cows with a low-moderate BCS submitted to a progesterone-based FTAI protocol with EB at implant removal.

Administering an additional prostaglandin F2α injection to Bos indicus beef cows during a treatment regimen for fixed-time artificial insemination

2020 ◽  
Vol 219 ◽  
pp. 106535
Author(s):  
Isabella M. Noronha ◽  
Reinaldo F. Cooke ◽  
Cícero F.G. Martins ◽  
Ramiro V. Oliveira Filho ◽  
Ky G. Pohler ◽  
...  
Keyword(s):  
Treatment Regimen ◽  
Artificial Insemination ◽  
Bos Indicus ◽  
Prostaglandin F2α ◽  
Fixed Time ◽  
Beef Cows
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