scholarly journals 72 Endocrine and ovarian responses to combined estradiol benzoate-sulpiride in anestrous mares treated with kisspeptin

2020 ◽  
Vol 98 (Supplement_2) ◽  
pp. 36-36
Author(s):  
Victoria N Bailey ◽  
Jennifer L Sones ◽  
Caroline M Camp ◽  
Erin L Oberhaus
Keyword(s):  
Repeated Measures ◽  
Estradiol Benzoate ◽  
Blood Sampling ◽  
Plasma Prolactin ◽  
Plasma Progesterone ◽  
Serial Blood ◽  
Osmotic Pumps ◽  
Pump Placement ◽  
Serial Blood Sampling ◽  
And Control

Abstract The objective of this study was to determine if incorporation of kisspeptin 10 (Kp10) into an estradiol benzoate (EB)-sulpiride treatment would result in greater endocrine responses, and a greater number of mares ovulating within 28 days of treatment compared to EB-sulpiride alone. Eighteen anestrous mares were blocked by horse type (light horse and pony crosses), body condition, and age, then randomly assigned to treatment or control. On day 0, all mares received 50 mg EB. On day 1, mini osmotic pumps containing saline (n = 9) or Kp10 (50 mg/hour; n = 9) were inserted subcutaneously in the neck and remained for 7 days. Serial blood sampling occurred for 24 hours after pump placement. On day 2, all mares received 3 g sulpiride. Serial blood sampling continued for 36 hours and daily for 28 days. Transrectal ultrasounds were performed regularly for detection of ovulation. Plasma was assayed for prolactin, luteinizing hormone (LH) and progesterone. Data were analyzed by ANOVA with repeated measures. Plasma prolactin increased (P < .001) in response to sulpiride in all mares and remained stimulated for 7 days. Prolactin responses tended to be greater (P = .09) in Kp10- treated mares compared to controls. No differences were detected in plasma LH during the first 24 hours after pump placement; however, LH increased in all mares beginning 5 days after sulpiride and were greater (P < .05) in Kp10-mares from day 7 to day 21. Eleven of 18 (61%) mares ovulated within 18 days of sulpiride treatment; however, no differences in ovulation dates were detected between Kp10 treated- and control- mares. No differences were detected in plasma progesterone during the first 5 days post ovulation. In conclusion, incorporation of Kp10 potentiated the prolactin and LH responses to EB-sulpiride, but did not further advance first ovulation in treated-mares.

Plasma prolactin concentrations during lactation, pouch young development and the return to behavioural oestrus in captive koalas (Phascolarctos cinereus)

2016 ◽  
Vol 28 (11) ◽  
pp. 1838
Author(s):  
K. Ballantyne ◽  
S. T. Anderson ◽  
A. Mucci ◽  
V. Nicolson ◽  
S. D. Johnston
Keyword(s):  
Blood Sampling ◽  
Plasma Prolactin ◽  
Early Lactation ◽  
Phascolarctos Cinereus ◽  
Releasing Hormone ◽  
Serial Blood ◽  
Serial Blood Sampling ◽  
Gonadotrophin Releasing Hormone ◽  
High Range

Plasma prolactin (PRL) concentrations in captive koalas during lactation were determined by serial blood sampling. PRL concentrations were low (1.3 ± 0.1 ng mL–1; n = 5) during early lactation until pouch young (PY) began to emerge from the pouch (around Day 130) before significantly (P < 0.05) increasing between Day 161 and Day 175 (5.3 ± 1.0 ng mL–1). A significant (P < 0.001) peak in PRL (7.7 ± 0.6 ng mL–1) coincided with maturing young between Day 189 and Day 231. All females failed to exhibit any signs of oestrous behaviour until Day 268.8 ± 8.5 (n = 4), some 102 ± 19 days before PY were weaned following achieving target weights of 2.5–2.7 kg. Throughout lactation, plasma LH concentrations were relatively high (range 4.9–8.7 ng mL–1) and LH responses to exogenous gonadotrophin-releasing hormone were observed in all koalas at all times during lactation.

Simple Cannulation Procedure for Serial Blood Sampling Through Cutaneous Ulnar Vein in Chickens

2012 ◽  
Vol 15 (1) ◽  
pp. 91-100 ◽  
Author(s):  
Darmel M. Bayer ◽  
K. Mohan ◽  
K. Jayakumar ◽  
Milad Manafi ◽  
B. H. Pavithra
Keyword(s):  
Blood Sampling ◽  
Serial Blood ◽  
Serial Blood Sampling

Effects of bromocriptine at parturition in the tammar wallaby, Macropus eugenii

1990 ◽  
Vol 2 (1) ◽  
pp. 79 ◽  
Author(s):  
TP Fletcher ◽  
G Shaw ◽  
MB Renfree
Keyword(s):  
Post Partum ◽  
Tammar Wallaby ◽  
Plasma Prolactin ◽  
Plasma Progesterone ◽  
Macropus Eugenii ◽  
Sequence Of Events ◽  
Prostaglandin Release ◽  
Peripartum Period ◽  
And Control ◽  
Subsequent Onset

Female tammar wallabies were treated with the dopamine agonist bromocriptine at the end of pregnancy to suppress the peripartum pulse of plasma prolactin. The animals were subsequently observed, and a series of blood samples taken to define the hormonal profiles before and immediately after parturition. Birth was observed in 4/5 control animals and occurred in 8/9 bromocriptine-treated animals. The peripartum peak in plasma PGFM concentrations was not affected by bromocriptine although the pulse of prolactin normally seen at parturition was completely abolished. The timing of luteolysis was apparently unaffected, as plasma progesterone concentrations fell similarly in both treated and control animals immediately after parturition. However, all of the neonates of the bromocriptine-treated animals died within 24 h, possibly because of a failure to establish lactation. Subsequent onset of post-partum oestrus was delayed or absent both in control and in bromocriptine-treated animals, suggesting that the frequent blood sampling and disturbances in the peripartum period interfered with these endocrine processes. It is concluded that both prolactin and prostaglandin can induce luteolysis in the pregnant wallaby, but that the normal sequence of events results from a signal of fetal origin inducing a prostaglandin release from the uterus, which in turn releases a pulse of prolactin that induces a progesterone decline.

A method for catheterization, harnessing and chronic infusion of undisturbed chickens

1989 ◽  
Vol 23 (3) ◽  
pp. 270-274 ◽  
Author(s):  
T. L. Cravener ◽  
R. Vasilatos-Younken
Keyword(s):  
Blood Sampling ◽  
Free Movement ◽  
Indwelling Catheter ◽  
Serial Blood ◽  
Chronic Infusion ◽  
Serial Blood Sampling ◽  
Meat Type

A system was designed to facilitate the chronic infusion and/or serial blood sampling of rapidly growing, meat-type chickens with little or no stress to the bird. Techniques for surgically preparing an indwelling catheter, fabricating a harness system to protect this catheter, utilizing a miniature fluid swivel to provide free movement and infusing solutions via a programmable pump are described.

scholarly journals Usefulness of Serial Blood Sampling and PCR Replicates for Treatment Monitoring of Patients with Chronic Chagas Disease

2018 ◽  
Vol 63 (2) ◽  
pp. e01191-18 ◽  
Author(s):  
Rudy Parrado ◽  
Juan Carlos Ramirez ◽  
Anabelle de la Barra ◽  
Cristina Alonso-Vega ◽  
Natalia Juiz ◽  
...  
Keyword(s):  
Treatment Failure ◽  
Chagas Disease ◽  
Sampling Strategy ◽  
Blood Sampling ◽  
Clinical Sensitivity ◽  
Serial Blood ◽  
The Third ◽  
Two Samples ◽  
Serial Blood Sampling ◽  
Chronic Chagas Disease

ABSTRACT This work evaluated a serial blood sampling procedure to enhance the sensitivity of duplex real-time quantitative PCR (qPCR) for baseline detection and quantification of parasitic loads and posttreatment identification of failure in the context of clinical trials for treatment of chronic Chagas disease, namely, DNDi-CH-E1224-001 (ClinicalTrials.gov registration no. NCT01489228) and the MSF-DNDi PCR Sampling Optimization Study (NCT01678599). Patients from Cochabamba (n = 294), Tarija (n = 257), and Aiquile (n = 220) were enrolled. Three serial blood samples were collected at each time point, and qPCR triplicates were tested for each sample. The first two samples were collected during the same day and the third one 7 days later. A patient was considered PCR positive if at least one qPCR replicate was detectable. Cumulative results of multiple samples and qPCR replicates enhanced the proportion of pretreatment sample positivity from 54.8% to 76.2%, 59.5% to 77.8%, and 73.5% to 90.2% in Cochabamba, Tarija, and Aiquile cohorts, respectively. This strategy increased the detection of treatment failure from 72.9% to 91.7%, 77.8% to 88.9%, and 42.9% to 69.1% for E1224 low-, short-, and high-dosage regimens, respectively, and from 4.6% to 15.9% and 9.5% to 32.1% for the benznidazole arm in the DNDi-CH-E1224-001 and MSF-DNDi studies, respectively. The addition of the third blood sample and third qPCR replicate in patients with nondetectable PCR results in the first two samples gave a small, non-statistically significant improvement in qPCR positivity. No change in clinical sensitivity was seen with a blood volume increase from 5 to 10 ml. The monitoring of patients treated with placebo in the DNDi-CH-E1224-001 trial revealed fluctuations in parasitic loads and occasionally nondetectable results. In conclusion, a serial sampling strategy enhanced PCR sensitivity to detecting treatment failure during follow-up and has the potential for improving recruitment capacity in Chagas disease trials, which require an initial positive qPCR result for patient admission.

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