Molecular characterization of plant acidic  -mannosidase, a member of glycosylhydrolase family 38, involved in the turnover of N-glycans during tomato fruit ripening

Virus-induced gene silencing (VIGS) is a powerful tool for the study of gene function and down regulate specific gene expression in plant. The RIN transcription factor is an important developmental regulator in tomato fruit ripening. In this work, the TRV-LeRIN infiltrated fruit significantly developed green color phenotypes, whereas the control fruit would turn red normally. Dissection of silenced areas for analysis identified whether the LeRIN gene is silenced or not. Taken together, we successfully applied syringe infiltration method of VIGS to silence the LeRIN gene in tomato fruit. These results are critical for understanding the mechanism of tomato fruit ripening.

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681 PB 195 MOLECULAR CHARACTERIZATION OF A cDNA THAT ENCODES 5-AMINOLEVULINIC ACID DEHYDRATASE IN TOMATO

HortScience ◽

10.21273/hortsci.29.5.530d ◽

1994 ◽

Vol 29 (5) ◽

pp. 530d-530

Author(s):

Gary F. Polkinz ◽

David J. Hannapel ◽

Richard J. Gladon

Keyword(s):

Fruit Ripening ◽

Aminolevulinic Acid ◽

Tomato Fruit ◽

Northern Analysis ◽

Chlorophyll Metabolism ◽

E Coli ◽

Acid Dehydratase ◽

Tomato Fruit Ripening

Tomato fruit ripening is characterized by a decrease in chlorophyll content and an increase in lycopene synthesis. We are interested in the role of chlorophyll metabolism as it relates to tomato fruit ripening. 5-Aminolevulinic acid dehydratase (ALAD) is the first committed enzyme in the chlorophyll biosynthetic pathway, and it catalyzes the conversion of two 5-aminolevulinic acid molecules into porphobilinogen. We have isolated a full-length tomato ALAD cDNA clone from a tomato fruit library. Sequence analysis showed that this tomato ALAD was highly homologous to ALAD found in spinach and pea, and the analysis predicted a protein of 46.8 kDa. Southern analysis indicated that 1 to 3 copies of the ALAD gene are present in the tomato genome. Northern analysis suggested that the gene is expressed constitutively throughout tomato fruit development. Currently, we are subcloning the fragment into an E. coli expression vector in order to obtain protein for antibody production for Western analysis.

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Changes in Photosynthetic Capacity and Photosynthetic Protein Pattern during Tomato Fruit Ripening

PLANT PHYSIOLOGY ◽

10.1104/pp.84.3.911 ◽

1987 ◽

Vol 84 (3) ◽

pp. 911-917 ◽

Cited By ~ 59

Author(s):

Birgit Piechulla ◽

Richard E. Glick ◽

Hubert Bahl ◽

Anastasios Melis ◽

Wilhelm Gruissem

Keyword(s):

Fruit Ripening ◽

Photosynthetic Capacity ◽

Protein Pattern ◽

Tomato Fruit ◽

Tomato Fruit Ripening

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Bio-speckle activity applied to the assessment of tomato fruit ripening

Biosystems Engineering ◽

10.1016/j.biosystemseng.2009.02.001 ◽

2009 ◽

Vol 103 (1) ◽

pp. 116-119 ◽

Cited By ~ 22

Author(s):

G.G. Romero ◽

C.C. Martinez ◽

E.E. Alanís ◽

G.A. Salazar ◽

V.G. Broglia ◽

...

Keyword(s):

Fruit Ripening ◽

Tomato Fruit ◽

Tomato Fruit Ripening

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Infection Strategies Deployed by Botrytis cinerea, Fusarium acuminatum, and Rhizopus stolonifer as a Function of Tomato Fruit Ripening Stage

Frontiers in Plant Science ◽

10.3389/fpls.2019.00223 ◽

2019 ◽

Vol 10 ◽

Cited By ~ 15

Author(s):

Stefan Petrasch ◽

Christian J. Silva ◽

Saskia D. Mesquida-Pesci ◽

Karina Gallegos ◽

Casper van den Abeele ◽

...

Keyword(s):

Botrytis Cinerea ◽

Fruit Ripening ◽

Tomato Fruit ◽

Rhizopus Stolonifer ◽

Ripening Stage ◽

Fusarium Acuminatum ◽

Tomato Fruit Ripening

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Ethylene Receptors, CTRs and EIN2 Target Protein Identification and Quantification Through Parallel Reaction Monitoring During Tomato Fruit Ripening

Frontiers in Plant Science ◽

10.3389/fpls.2018.01626 ◽

2018 ◽

Vol 9 ◽

Cited By ~ 13

Author(s):

Clara I. Mata ◽

Bertrand Fabre ◽

Harriet T. Parsons ◽

Maarten L. A. T. M. Hertog ◽

Geert Van Raemdonck ◽

...

Keyword(s):

Fruit Ripening ◽

Protein Identification ◽

Tomato Fruit ◽

Target Protein ◽

Reaction Monitoring ◽

Parallel Reaction ◽

Ethylene Receptors ◽

Parallel Reaction Monitoring ◽

Tomato Fruit Ripening ◽

Identification And Quantification

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Potato Virus X-induced LeHB-1 Silencing Delays Tomato Fruit Ripening

Journal of the American Society for Horticultural Science ◽

10.21273/jashs04497-18 ◽

2018 ◽

Vol 143 (6) ◽

pp. 454-461 ◽

Cited By ~ 1

Author(s):

Xiaohong Wang ◽

Bishun Ye ◽

Xiangpeng Kang ◽

Ting Zhou ◽

Tongfei Lai

Keyword(s):

Potato Virus ◽

Fruit Ripening ◽

Ph Value ◽

Tomato Fruit ◽

Potato Virus X ◽

Anthocyanin Content ◽

Genetic Trait ◽

Tomato Fruit Ripening ◽

Homeobox Protein ◽

Complex Genetic Trait

Tomato (Solanum lycopersicum) fruit ripening is a complex genetic trait correlating with notable fruit phenotypic, physiologic, and biochemical changes. Transcription factors (TFs) play crucial roles during this process. LeHB-1, an HD-zip homeobox protein, is a ripening-related TF and acts as an important regulator of fruit ripening. However, the detailed biochemical and molecular basis of LeHB-1 on tomato fruit ripening is unclear. In the current study, the biologic functions of LeHB-1 were determined by a potato virus X (PVX)-mediated gene-silencing approach. The results indicate that PVX-induced LeHB-1 silencing in tomato could decrease pigment accumulation and delay fruit ripening. Compared with controls, nonripening flesh retains a greater pH value and a lesser anthocyanin content. By evaluating expression levels of genes related to tomato fruit ripening, we inferred that LeHB-1 located at the downstream of LeMADS-RIN-mediated regulatory network. In addition, LeHB-1 silencing mainly disturbed phytoene desaturation and isomerization, and led to a decrease in trans-lycopene accumulation, but did not influence flavonoid biosynthesis directly in tomato fruit. The findings provide a theoretical foundation for illustrating the biologic functions of LeHB-1 in tomato fruit ripening and quality.

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