Embryo development of hatching eggs of superior local broiler (ALPU) with Arab native chickens (Kamaras)

Efforts to improve the quality of local chickens, especially free-range chickens, are carried out by improving genetic traits, namely through a strict selection method to produce broilers with the name of Superior Local Broilers (ALPU) and laying purposes with Arab Kampung Chickens (Kamaras). This study was conducted to obtain information on the comparison of ALPU and Kamaras embryo development for genetic improvement as the basis for further research. This study used 105 ALPU hatching eggs and 105 Kamaras hatching eggs, the observations were carried out for 21 days. Eggs were collected for 7 days and then put into the incubator, then observed every day by breaking each 5 ALPU and Kamaras eggs to see 7 parameters of embryo development in the form of: body weight, body length, head circumference, neck length, beak length, wing length and leg length. The results showed that there was no significant developmental difference between ALPU and Kamaras embryos. However, the embryo weight and beak length of Kamaras tended to be larger than those of ALPU, while the head circumference and wing length of ALPU tended to be larger than that of Kamaras. In conclusion, the embryonic development between ALPU and Kamaras showed relatively similar embryo development during the hatching period.

Hatching, embryo death and chicks growth in dual purpose and brawl hens under natural hypobaric conditions

The present study aimed to compare results for hatchability and growing performance of chicks from 2 biotypes, dual purpose (DP) and brawl (BR), in hipobaric conditions (2718 m above sea level). In experiment 1, a total of 352 hatching eggs were randomly arranged, 176 egg of DP y 176 BR. In experiment 2, a total of 114 one-day-old unsexed chickens were reared for 28 d. The experimental design for hatchability and chick performance variables was completely randomized with 2 treatments (DP and BR). There were no differences between the studied treatments for hatchability results (p>0.05). Hens’ biotype affected chicken performance in the rearing phase (p<0.01), where the DP chickens had better body weight and feed conversion than BR, but BR chickens had lower mortality than DP chickens.

Effect of Storage Time On the Hatchability of Eggs of Two-Line Dual-Purpose Combination for Production of Male Chickens for Meat

The aim of the study was to assess the effect of the storage time on the hatching traits of eggs obtained from a two-line dual-purpose combination for production of male chickens that will be utilized for meat. The trial was carried out in the experimental poultry farm of the Institute of Animal Science-Kostinbrod, with a total of 150 Lohmann Brown Classic layers, at the age of 54 weeks. Hatching eggs were gathered for three weeks and were stored for 5, 10 and 15 days at 15-18°C ambient temperature prior incubation. The storage time had no effect on the fertility of the set eggs, however, it adversely affected the hatchability. Prolonged storage led to significant decline in the total hatchability (P=0.0027) and the hatchability of the fertile eggs (P<0.0001) which was lowest after 15 days of storage prior incubation. The viability of the chickens was influenced by the storage of the eggs prior incubation (P<0.0001), and decreased considerably when the chicks were hatched from eggs stored for 15 days.

Impact of Treating Hatching Eggs with Curcumin after Exposure to Thermal Stress on Embryonic Development, Hatchability, Physiological Body Reactions, and Hormonal Profiles of Dokki-4 Chickens

This study evaluated the impact of incubation temperature and spraying hatching eggs with curcumin during the early embryogenesis phase on chick embryo developments, hatchability, physiological body reactions, and hormonal profiles of Dokki 4 chickens. A total of 720 fertile eggs were equally distributed into two groups. In the first group, the eggs were incubated at normal incubation temperature/NIT (37.8 °C and 55–60% RH) for up to 19 days of incubation, whereas those in the second group were incubated in the same conditions except from 6 to 8 day, in which they were daily exposed to chronic incubation temperature/CIT (39.0 °C) for 3 h. Each group was classified into four curcumin treatment doses; the 1st treatment (control) was sprayed with distilled water, while the 2nd, 3rd, and 4th treatments were sprayed with 250, 500, and 1000 mg curcumin/liter distilled water. The results indicated that the lowest hatchability of fertile eggs (%) was obtained in the CIT group (p = 0.02), whereas the highest body surface temperature/BST compared in the NIT group (p = 0.01). Regarding curcumin treatments, the percentages of heart, gizzard, spleen, and T3 hormone levels in the treated group were significantly increased, while the H/L ratio was significantly reduced (p = 0.001) compared with the control. At 8 weeks of age, the testes and ovary percentages in treated groups were significantly (p = 0.05) increased compared with the control. In conclusion, exposure of hatching eggs to high thermal stress (39 °C) during the incubation phase had deleterious effects on chick performance and T3 hormone level. Moreover, spraying hatching eggs had beneficial impacts on growth, reproductive organs, T3 hormone level, and reducing H/L ratio.

Comparative Analysis The Effect of Iodine, Ozone, and Peracetic Acid as A Disinfectant of Cantang Grouper Egg

Problems in the fish hatchery sector often occur such as high egg mortality or low quality of fish eggs. Optimizing biosecurity is one of the efforts to improve the quality of fish eggs, for example by adding disinfectants such as iodine, ozone, and peracetic acid. The use of chemical disinfectants will produce good results if the right dose, time, and commodity are used. In this study. This research aims (1) to determine whether the use of chemicals including iodine, ozone, and peracetic acid had an effect as a disinfectant to increase the hatching rate of grouper fish, (2) to determine the level of effectiveness and efficiency of the use of chemicals as a disinfectant. This research is an experimental research type. The research subjects included in the study were cantang grouper fish eggs at PT. Pakarti Daksa Segara who used the exploratory sampling method. The conclusion of this study indicates that there is an influence on the growth rate of the use of iodine, ozone and, peracetic acid chemicals as disinfectants of cantang grouper eggs and there is a comparison of the effectiveness and efficiency of the use of the three chemicals which includes the percentage of the hatching rate, the cost of the disinfectant material and the time, which is needed in the use of the disinfectant. Iodine has the best level of effectiveness from the comparison of hatching eggs of cantang grouper and peracetic acid is the most efficient type of chemical.

Occurrence, Risk Factors, Serotypes and Antimicrobial Resistance of Salmonella Strains Isolated from Imported Fertile Hatching Eggs, Hatcheries, and Broiler Farms in Trinidad and Tobago

This cross-sectional study determined the occurrence, risk factors and characteristics of Salmonella isolated from imported broiler hatching fertile eggs, hatcheries, and broiler farms in Trinidad and Tobago. Standard methods were used to isolate and characterize Salmonella isolates from two broiler hatcheries and 27 broiler farms in the country. Salmonella was isolated from 0.0% (0/45), 7.6% (12/158) and 2.8% (24/866) of fertile hatching eggs (composite of 450), hatcheries and broiler farms, respectively (p=0.006). The highest frequency of Salmonella isolation was detected in stillborn chicks, 28.0% (7/28) and in cloacal swabs, 2.2% (15/675) in samples collected from the hatcheries and farms, respectively. None of the 15 farm management and production practices investigated was significantly (P&gt;0.05) associated with the isolation of Salmonella . The predominant serotypes among Salmonella were Kentucky (83.3%) and Infantis (62.5%) for hatchery and farms isolates, respectively. The disc diffusion method revealed a frequency of antimicrobial-resistant Salmonella of 44.0% (11/25) and 87.5% (35/40) in isolates from the hatcheries and broiler farms, respectively (p=0.0002). Antimicrobial resistance was highest (28.0%) to doxycycline and kanamycin for hatchery isolates, while for farm isolates resistance was high (&gt;65%) to sulphamethoxazole-trimethoprim, gentamycin, ceftriaxone, kanamycin, and doxycycline. Multi-drug resistance (MDR) was exhibited by 4.0% and 85.7% of Salmonella isolates from the hatcheries and farms, respectively (p&lt;0.0001). The high antimicrobial resistance and MDR exhibited by Salmonella from broiler farms highlight the therapeutic implications and the potential to enter the food chain.

Effect of Pre-Storage Heating and Period of Storage on Hatchability Traits of Dokki-4 Eggs

This study investigated the effects of pre-storage heating and storage period of hatching eggs on hatchability traits and chick quality of Dokki-4 (Egyptian local strain of chickens) laying hens. A total of 3600 eggs were collected from 46-week-old laying hens. Eggs were distributed in a 3x4 factorial arrangement, with three storage times (4, 8 and 12 days at 18°C and 75% RH) and four heat treatments prior to storage (0, 3, 6 and 9 hours at 37.5°C and 56% RH). Eggs were distributed to twelve treatments of 20 replicates. After storage, eggs were incubated under the normal conditions of incubation at the same time. The results showed that the long storage period increased egg weight loss. Hatchability and chick quality results from 8-12 days stored eggs were lower than eggs stored for 4 days. The 6-hour pre-storage heating system substantially improved egg hatchability and chick quality relative to non-heated or 9-hour heating. Important interactions were observed during pre-storage heating × egg storage time for loss in egg weight, hatchability of total and fertile eggs, embryonic mortality and chick quality. When eggs were stored for more than four days, pre-storage heating of hatching eggs for six hours improved hatchability and chick quality compared to unheated eggs or heated for 9 hours. Conclusively, pre-storage heat treatment beneficially affects hatchability traits and chick quality, especially when hatching eggs are stored for long periods.

PSVIII-20 Heterologous lactobacilli feed additive impact on microflora in Cobb-500 broiler laying hens

Experiment was carried out on introducing eggshells from an experimental group of laying hens of lactobacilli strains with anti-C.jejuni activity. Eggs were incubated for 21 days to obtain broiler chickens containing auto-strains of lactobacilli with anti-C.jejuni activity in the blind processes of the intestine. It should be noted that most of the incubation eggs were observed in the 2nd group, in which the eggs were laid for subsequent administration of probiotic bacteria. Check was carried out for the presence of auto-strains of lactobacilli with anti-C.jejuni activity in the intestines of broiler chickens and the timing of their persistence for 1–35 days. The objective of the study was a comparative analysis of the following DNA samples: DNA from a feed supplement containing strains of lactobacilli with anti-C.jejuni activity; DNA samples from litter of laying hens of the parent herd receiving heterologous lactobacilli as part of the feed; isolated DNA from washes from hatching eggs; DNA samples from the contents of the digestive tract of chicken embryos; DNA samples from the intestines of broiler chickens. DNA samples from groups of birds and their embryos obtained without the use of heterologous lactobacilli were used as controls. The microflora of the contents of blind processes was taken from chickens on the 22nd day and on the 35th day of growing. In total, 343 different bacterial OTUs were identified in this study, belonging to 13 types, 26 classes, 51 orders, 106 families and 172 genera. Assessment of the biodiversity of bacterial ensembles in the samples of the contents of blind processes of chickens revealed fundamental differences already at the level of growing time and type. The study was performed at the FGBOU VO “St. Petersburg State University of Veterinary Medicine» with the aid of the Russian Science Foundation Grant (Project No. 18-76-10017).