scholarly journals Detection of Novel Spotted Fever Group Rickettsiae (Rickettsiales: Rickettsiaceae) in Ticks (Acari: Ixodidae) in Southwestern China

2021 ◽  
Author(s):  
Qian Wang ◽  
Wen-Bin Guo ◽  
Yu-Sheng Pan ◽  
Bao-Gui Jiang ◽  
Chun-Hong Du ◽  
...  
Keyword(s):  
Novel Species ◽  
Phylogenetic Analyses ◽  
Spotted Fever ◽  
Southwestern China ◽  
Spotted Fever Group ◽  
Haemaphysalis Longicornis ◽  
Spotted Fever Group Rickettsiae ◽  
Vector Distribution ◽  
Etiological Agents ◽  
And Control

Abstract Spotted fever group rickettsiae, mainly maintained and transmitted by ticks, are important etiological agents of (re)emerging zoonotic diseases worldwide. It is of great significance to investigate spotted fever group rickettsiae in ticks in different areas for the prevention and control of rickettsioses. In this study, a total of 305 ticks were collected from wild and domestic animals in Chongqing, Guizhou, Yunnan, and Guangxi provinces of southwestern China during 2017–2019 and examined for the presence of spotted fever group rickettsiae by PCR with primers targeting the partial gltA, ompA, rrs, and htrA genes. Results showed that two spotted fever group rickettsiae species, including the pathogenic Candidatus Rickettsia jingxinensis (Rickettsiales: Rickettsiaceae) and a potential novel species Rickettsia sp. sw (Rickettsiales: Rickettsiaceae), were identified. The Ca. R. jingxinensis sequences were recovered from Rhipicephalus microplus (Ixodida: Ixodidae) and Haemaphysalis longicornis (Ixodida: Ixodidae) ticks and phylogenetically clustered with previous Ca. R. jingxinensis, Ca. R. longicornii (Rickettsiales: Rickettsiaceae), and Rickettsia sp. XY118 (Rickettsiales: Rickettsiaceae) strains. Rickettsia sp. sw was detected in Amblyomma geoemydae (Ixodida: Ixodidae) and Rh. microplus. Interestingly, as far as we know, this was the first report of Rickettsia (Rickettsiales: Rickettsiaceae) in A. geoemydae. Phylogenetic analyses indicated that this potential novel species was closely related to R. aeschlimannii (Rickettsiales: Rickettsiaceae) with gltA and ompA genes and grouped in a cluster composed of R. montanensis (Rickettsiales: Rickettsiaceae), R. raoultii (Rickettsiales: Rickettsiaceae), R. aeschlimannii, R. massiliae (Rickettsiales: Rickettsiaceae), and R. rhipicephali (Rickettsiales: Rickettsiaceae) with htrA, while formed a separate clade with rrs. The pathogenicity of Rickettsia sp. sw should be further confirmed. These results expand the knowledge of the geographical distribution and vector distribution of spotted fever group rickettsiae in China and are useful for assessing the potential public health risk.

scholarly journals First report of the molecular detection of human pathogen Rickettsia raoultii in ticks from the Republic of Korea

2021 ◽  
Vol 14 (1) ◽  
Author(s):  
Misbah Tariq ◽  
Jun-Won Seo ◽  
Da Young Kim ◽  
Merlin Jayalal Lawrence Panchali ◽  
Na Ra Yun ◽  
...  
Keyword(s):  
Dna Sequences ◽  
Citrate Synthase ◽  
Phylogenetic Analyses ◽  
Spotted Fever ◽  
Republic Of Korea ◽  
Spotted Fever Group ◽  
Haemaphysalis Longicornis ◽  
Rickettsia Raoultii ◽  
Rickettsial Infections ◽  
The Republic

Abstract Background Rickettsial diseases associated with the spotted fever group constitute a growing number of newly identified Rickettsia pathogens and their tick vectors in various parts of the world. At least 15 distinct tick species belonging to six genera have shown the presence of Rickettsia raoultii. Herein, we report the detection of R. raoultii in ticks from the Republic of Korea (ROK). Methods Thirty-five ticks were collected from 29 patients with tick bites in Gwangju Metropolitan City, Jeollanam Province, ROK. The ticks were identified using molecular, morphological, and taxonomic characteristics. All samples were screened for presence of Rickettsia species using nested polymerase chain reactions of their outer membrane protein (ompA) and citrate synthase (gltA) genes. The amplified products were sequenced for subsequent phylogenetic analyses. Results Sequencing data showed the DNA sequences of R. raoultii in three Haemaphysalis longicornis ticks. All three tick samples were 99.4–100% similar to previously reported partial sequences of ompA of R. raoultii strains CP019435 and MF002523, which formed a single clade with the reference strains. Conclusions We provide the first description and molecular identification of R. raoultii detected in H. longicornis ticks in the ROK. This observation extends the geographical distribution of R. raoultii. Screening of human samples for this pathogen will provide information about the prevalence of rickettsial infections in this region.

scholarly journals Spotted fever group rickettsiae (SFGR) detection in ticks following reported human case of Japanese spotted fever in Niigata Prefecture, Japan

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Reiko Arai ◽  
Megumi Sato ◽  
Miwako Kato ◽  
Junko Aoki ◽  
Akiko Nishida ◽  
...  
Keyword(s):  
Public Awareness ◽  
Spotted Fever ◽  
Human Case ◽  
Ixodes Persulcatus ◽  
Spotted Fever Group ◽  
Haemaphysalis Longicornis ◽  
Spotted Fever Group Rickettsiae ◽  
Niigata Prefecture ◽  
Health Initiatives ◽  
Tick Borne Disease

AbstractJapanese spotted fever, a tick-borne disease caused by Rickettsia japonica, was firstly described in southwestern Japan. There was a suspicion of Rickettsia japonica infected ticks reaching the non-endemic Niigata Prefecture after a confirmed case of Japanese spotted fever in July 2014. Therefore, from 2015 to 2017, 38 sites were surveyed and rickettsial pathogens were investigated in ticks from north to south of Niigata Prefecture including Sado island. A total of 3336 ticks were collected and identified revealing ticks of three genera and ten species: Dermacentor taiwanensis, Haemaphysalis flava, Haemaphysalis hystricis, Haemaphysalis longicornis, Haemaphysalis megaspinosa, Ixodes columnae, Ixodes monospinosus, Ixodes nipponensis, Ixodes ovatus, and Ixodes persulcatus. Investigation of rickettsial DNA showed no ticks infected by R. japonica. However, three species of spotted fever group rickettsiae (SFGR) were found in ticks, R. asiatica, R. helvetica, and R. monacensis, confirming Niigata Prefecture as a new endemic area to SFGR. These results highlight the need for public awareness of the occurrence of this tick-borne disease, which necessitates the establishment of public health initiatives to mitigate its spread.

scholarly journals Isolation of spotted fever group rickettsiae from Ixodes persulcatus ticks in the central part of Hokkaido, Japan

2000 ◽  
Vol 51 (1) ◽  
pp. 55-58 ◽  
Author(s):  
Hiromi FUJITA ◽  
Nobuhiro TAKADA ◽  
Emiko ISOGAI ◽  
Yuriko WATANABE ◽  
Takuya ITO
Keyword(s):  
Spotted Fever ◽  
Ixodes Persulcatus ◽  
Spotted Fever Group ◽  
Spotted Fever Group Rickettsiae

scholarly journals Seroepidemiologic Study of Human Infections with Spotted Fever Group Rickettsiae in North Carolina

2014 ◽  
Vol 52 (11) ◽  
pp. 3960-3966 ◽  
Author(s):  
M. F. Vaughn ◽  
J. Delisle ◽  
J. Johnson ◽  
G. Daves ◽  
C. Williams ◽  
...  
Keyword(s):  
North Carolina ◽  
Spotted Fever ◽  
Spotted Fever Group ◽  
Spotted Fever Group Rickettsiae ◽  
Human Infections ◽  
Seroepidemiologic Study

scholarly journals Spotted Fever Group Rickettsiae in Inner Mongolia, China, 2015–2016

2018 ◽  
Vol 24 (11) ◽  
pp. 2105-2107 ◽  
Author(s):  
Xuhong Yin ◽  
Shengchun Guo ◽  
Chunlian Ding ◽  
Minzhi Cao ◽  
Hiroki Kawabata ◽  
...  
Keyword(s):  
Inner Mongolia ◽  
Spotted Fever ◽  
Spotted Fever Group ◽  
Spotted Fever Group Rickettsiae

scholarly journals Classification of Tick Species and Detection of Tick-borne Pathogens in Yanbian, China

2020 ◽  
Author(s):  
Jixu Li ◽  
Shuang Zhang ◽  
Wanfeng Liang ◽  
Shaowei Zhao ◽  
Hao Wang ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
North Korea ◽  
Tick Species ◽  
Spotted Fever ◽  
Ixodes Persulcatus ◽  
Spotted Fever Group ◽  
Haemaphysalis Longicornis ◽  
Chain Reaction ◽  
Polymerase Chain ◽  
Severe Fever

Abstract BackgroundYanbian is located at the junction between China, Russia, and North Korea. We aimed to determine the species distribution and pathogens carried by ticks in Yanbian.MethodsA total of 2673 unattached ticks were collected from eight counties and cities in Yanbian and classified morphologically. Candidatus Rickettsia tarasevichiae (CRT), spotted fever group Rickettsia (SFGR), severe fever thrombocytopenia syndrome virus (SFTSV), Theileria, and other pathogens were detected using polymerase chain reaction (PCR) and real-time quantitative polymerase chain reaction followed by phylogenetic and genotypic analyses.ResultsAccording to the morphological classification, the main tick species in Yanbian were Haemaphysalis longicornis, Ixodes persulcatus, Dermacentor silvarum, Haemaphysalis japonica, and Haemaphysalis concinna. Candidatus Rickettsia tarasevichiae, spotted fever group Rickettsia, severe fever thrombocytopenia syndrome virus, and Theileria orientalis were detected in H. longicornis, Candidatus Rickettsia tarasevichiae, spotted fever group Rickettsia, and severe fever thrombocytopenia syndrome virus were detected in I. persulcatus, H. japonica, and D. silvarum, but only severe fever thrombocytopenia syndrome virus was detected in H. concinna. Mixed infection with Candidatus Rickettsia tarasevichiae and severe fever thrombocytopenia syndrome virus was found in I. persulcatus and H. japonica. The gene sequences of all tested pathogens exhibited 95.7%–100% homology with sequences registered in GenBank. Phylogenetic analysis showed that different spotted fever group Rickettsia and severe fever thrombocytopenia syndrome virus genotypes were closely related to the Korean strains. We provide the first evidence for the presence of the spotted fever group Rickettsia genotypes of Candidatus Rickettsia longicornii, ompA, ompB, sca4, and rrs, in Haemaphysalis longicornis in Yanbian. ConclusionsThese results provide epidemiological data to support the prevention and control of ticks and tick-borne diseases in the border areas of China, North Korea, and Russia.

New records of spotted fever group rickettsiae in Baltic region

2015 ◽  
Vol 17 (11-12) ◽  
pp. 874-878 ◽  
Author(s):  
Jana Radzijevskaja ◽  
Algimantas Paulauskas ◽  
Asta Aleksandraviciene ◽  
Indre Jonauskaite ◽  
Michal Stanko ◽  
...  
Keyword(s):  
New Records ◽  
Spotted Fever ◽  
Spotted Fever Group ◽  
Baltic Region ◽  
Spotted Fever Group Rickettsiae
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