Micronutrient Assay for Cancer Prevention Clinical Trials: Serum Retinol, Retinyl Palmitate, Alpha-Carotene, and Beta-Carotene With the Use of High-Performance Liquid Chromatography2

1987 ◽  
Keyword(s):  
Clinical Trials ◽  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Cancer Prevention ◽  
High Performance ◽  
Beta Carotene ◽  
Retinyl Palmitate ◽  
Serum Retinol

scholarly journals Simultaneous determination of retinol, retinyl palmitate and β-carotene in rat serum treated with 7,12-dimethylbenz[a]anthracene and Hypericum Perforatum L. by high-performance liquid chromatography with diode-array detection

2010 ◽  
Vol 8 (1) ◽  
pp. 108-115 ◽  
Author(s):  
Abdulkadir Levent ◽  
Suat Ekin ◽  
Gökhan Oto
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Hypericum Perforatum ◽  
Treated Group ◽  
Retinyl Palmitate ◽  
Rat Serum ◽  
Array Detection ◽  
Hypericum Perforatum L ◽  
Β Carotene

AbstractA new and simple high-performance liquid chromatography method was developed and validated for the simultaneous determination of retinol, retinyl palmitate and β-carotene in rat serum treated with Hypericum Perforatum L. and 7,12-dimethylbenz[a]anthracene. Furthermore, vitamin C was determined spectrophotometrically. High-performance liquid chromatography analysis was performed utilizing an Inertsil ODS3 reversed phase column with methanol-acetonitrile-tetrahydrofuran (65:30:5, v/v/v) as mobile phase, at a flow rate of 1.5 mL min−1 and 40°C. Diode-array detection was conducted at 325 and 450 nm for retinol and retinyl palmitate, and β-carotene, respectively with a running time of 26 min. The high-performance liquid chromatography assay and extraction procedure proposed are simple, rapid, sensitive and accurate. This method was then applied to determine the amounts of retinol, retinyl palmitate and β-carotene in rat serum. Results of this study demonstrated that at 60th day in the 7,12-dimethylbenz[a]anthracene-treated group there was a significant decrease (pa] anthracene + Hypericum Perforatum L. treated group compared to the control group..

Determination of beta-carotene and its cis isomers in serum

1990 ◽  
Vol 36 (11) ◽  
pp. 1986-1989 ◽  
Author(s):  
W G Rushin ◽  
G L Catignani ◽  
S J Schwartz
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Human Serum ◽  
High Performance ◽  
Beta Carotene ◽  
Reversed Phase ◽  
Serum Samples ◽  
Analytical Recovery ◽  
Cis Isomer

Abstract All-trans-beta-carotene was resolved from its cis isomers in human serum by reversed-phase "high-performance" liquid chromatography. Absorption spectra of the cis peak suggested that 13-cis-beta-carotene was the predominant cis isomer. Analyses and recovery studies of fresh and stored sera eliminated the possibility that isomerization had occurred in samples during handling or storage. The average analytical recovery was 101.9% for standards of the all-trans-, 9-cis-, and 13-cis-beta-carotene compounds in pooled serum samples. We also demonstrated that cis isomers had not formed after the blood was drawn and that cis isomers of beta-carotene are present at significant concentrations in the human circulation.

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